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Product Info Summary
| SKU: | M00338 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-BMP2 Rabbit Monoclonal Antibody
SKU/Catalog Number
M00338
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-BMP2 Rabbit Monoclonal Antibody catalog # M00338. Tested in WB application. This antibody reacts with Human, Mouse.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-BMP2 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00338)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
AOHD-2
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human BMP2
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M00338 is reactive to BMP2 in Human, Mouse
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
32 kDa
Calculated molecular weight
44702 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00338 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
Positive Control
WB: HeLa cell
Validation Images & Assay Conditions
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Western blot analysis of BMP2 expression in HeLa cell lysate.
Protein Target Info & Infographic
Gene/Protein Information For BMP2 (Source: Uniprot.org, NCBI)
Gene Name
BMP2
Full Name
Bone morphogenetic protein 2
Weight
44702 MW
Superfamily
TGF-beta family
Alternative Names
Bone morphogenetic protein 2;BMP-2;Bone morphogenetic protein 2A;BMP-2A;BMP2;BMP2A; BMP2 BDA2A, SSFSC, SSFSC1, BMP2 bone morphogenetic protein 2 bone morphogenetic protein 2|bone morphogenetic protein 2A
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on BMP2, check out the BMP2 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for BMP2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-BMP2 Rabbit Monoclonal Antibody (M00338)
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Customer Q&As
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13 Customer Q&As for Anti-BMP2 Rabbit Monoclonal Antibody
Question
Is a blocking peptide available for product anti-BMP2 Rabbit Monoclonal antibody (M00338)?
Verified Customer
Verified customer
Asked: 2020-03-05
Answer
We do provide the blocking peptide for product anti-BMP2 Rabbit Monoclonal antibody (M00338). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2020-03-05
Question
Is this M00338 anti-BMP2 Rabbit Monoclonal antibody reactive to the isotypes of BMP2?
Verified Customer
Verified customer
Asked: 2019-11-13
Answer
The immunogen of M00338 anti-BMP2 Rabbit Monoclonal antibody is A synthesized peptide derived from human BMP2. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-11-13
Question
We are currently using anti-BMP2 Rabbit Monoclonal antibody M00338 for human tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human. Is it true that the antibody can work on zebrafish tissues as well?
Verified Customer
Verified customer
Asked: 2019-11-07
Answer
The anti-BMP2 Rabbit Monoclonal antibody (M00338) has not been validated for cross reactivity specifically with zebrafish tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in zebrafish you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-11-07
Question
I was wanting to use your anti-BMP2 Rabbit Monoclonal antibody for WB for human thyroid gland on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human thyroid gland identification?
S. Kulkarni
Verified customer
Asked: 2019-09-12
Answer
As indicated on the product datasheet, M00338 anti-BMP2 Rabbit Monoclonal antibody has been tested for WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human thyroid gland in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-09-12
Question
Will anti-BMP2 Rabbit Monoclonal antibody M00338 work for WB with thyroid gland?
Verified Customer
Verified customer
Asked: 2019-07-11
Answer
According to the expression profile of thyroid gland, BMP2 is highly expressed in thyroid gland. So, it is likely that anti-BMP2 Rabbit Monoclonal antibody M00338 will work for WB with thyroid gland.
Boster Scientific Support
Answered: 2019-07-11
Question
Thank you for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for thyroid gland using anti-BMP2 Rabbit Monoclonal antibody M00338. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-06-06
Answer
Thank you for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-06-06
Question
Do you have a BSA free version of anti-BMP2 Rabbit Monoclonal antibody M00338 available?
Verified Customer
Verified customer
Asked: 2018-02-05
Answer
I appreciate your recent telephone inquiry. I can confirm that some lots of this anti-BMP2 Rabbit Monoclonal antibody M00338 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2018-02-05
Question
I have a question about product M00338, anti-BMP2 Rabbit Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2018-01-25
Answer
We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M00338 anti-BMP2 Rabbit Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2018-01-25
Question
I see that the anti-BMP2 Rabbit Monoclonal antibody M00338 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2017-09-14
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2017-09-14
Question
Our lab want to know about using your anti-BMP2 Rabbit Monoclonal antibody for regulation of apoptotic process studies. Has this antibody been tested with western blotting on hela cell lysate? We would like to see some validation images before ordering.
B. Edwards
Verified customer
Asked: 2017-06-07
Answer
I appreciate your inquiry. This M00338 anti-BMP2 Rabbit Monoclonal antibody is validated on hela cell lysate. It is guaranteed to work for WB in human. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2017-06-07
Question
I am interested in to test anti-BMP2 Rabbit Monoclonal antibody M00338 on human thyroid gland for research purposes, then I may be interested in using anti-BMP2 Rabbit Monoclonal antibody M00338 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
C. Evans
Verified customer
Asked: 2016-12-26
Answer
The products we sell, including anti-BMP2 Rabbit Monoclonal antibody M00338, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2016-12-26
Question
I have attached the WB image, lot number and protocol we used for thyroid gland using anti-BMP2 Rabbit Monoclonal antibody M00338. Please let me know if you require anything else.
Z. Gonzalez
Verified customer
Asked: 2016-12-22
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2016-12-22
Question
Will M00338 anti-BMP2 Rabbit Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
T. Dhar
Verified customer
Asked: 2016-06-01
Answer
It shows on the product datasheet, M00338 anti-BMP2 Rabbit Monoclonal antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2016-06-01
