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Product Info Summary
| SKU: | A04449-1 |
|---|---|
| Size: | 100μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IHC, WB |
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Product info
Product Name
Anti-BMP-3A Antibody
SKU/Catalog Number
A04449-1
Size
100μl
Form
Liquid
Description
Boster Bio Anti-BMP-3A Antibody catalog # A04449-1. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-BMP-3A Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A04449-1)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
The antiserum was produced against synthesized peptide derived from the C-terminal region of human BMP3. AA range:421-470
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A04449-1 is reactive to BMP3 in Human, Mouse, Rat
Reconstitution
Observed Molecular Weight
39 kDa
Calculated molecular weight
53372 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A04449-1 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB, 1:500-1:2000
IHC-P, 1:100-1:300
Validation Images & Assay Conditions
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Western Blot (WB) analysis of Jurkat cells using BMP-3A Polyclonal antibody.
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Immunohistochemistry (IHC) analysis of paraffin-embedded Human Liver Cancer, antibody was diluted at 1:100.
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Immunohistochemistry (IHC) analysis of paraffin-embedded Human Liver Cancer, antibody was diluted at 1:100.
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Immunohistochemistry (IHC) analysis of paraffin-embedded Mouse Muscle, antibody was diluted at 1:100.
Protein Target Info & Infographic
Gene/Protein Information For BMP3 (Source: Uniprot.org, NCBI)
Gene Name
BMP3
Full Name
Bone morphogenetic protein 3
Weight
53372 MW
Superfamily
TGF-beta family
Alternative Names
Bone morphogenetic protein 3;BMP-3;Bone morphogenetic protein 3A;BMP-3A;Osteogenin;BMP3;BMP3A; BMP3 BMP-3A bone morphogenetic protein 3 bone morphogenetic protein 3|bone morphogenetic protein 3 (osteogenic)|bone morphogenetic protein 3A|osteogenin
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on BMP3, check out the BMP3 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for BMP3: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-BMP-3A Antibody (A04449-1)
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No publications found for A04449-1
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Customer Q&As
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13 Customer Q&As for Anti-BMP-3A Antibody
Question
Is this A04449-1 anti-BMP-3A antibody reactive to the isotypes of BMP3?
Verified Customer
Verified customer
Asked: 2020-05-07
Answer
The immunogen of A04449-1 anti-BMP-3A antibody is Synthesized peptide derived from the C-terminal region of human BMP-3A. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2020-05-07
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for lung using anti-BMP-3A antibody A04449-1. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2020-05-04
Answer
Thanks for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-05-04
Question
Will A04449-1 anti-BMP-3A antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2020-02-14
Answer
As indicated on the product datasheet, A04449-1 anti-BMP-3A antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2020-02-14
Question
I was wanting to use your anti-BMP-3A antibody for WB for rat lung on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for rat lung identification?
Verified Customer
Verified customer
Asked: 2020-02-07
Answer
As indicated on the product datasheet, A04449-1 anti-BMP-3A antibody has been tested for IHC, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in rat lung in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2020-02-07
Question
We are currently using anti-BMP-3A antibody A04449-1 for mouse tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on pig tissues as well?
Verified Customer
Verified customer
Asked: 2019-09-26
Answer
The anti-BMP-3A antibody (A04449-1) has not been validated for cross reactivity specifically with pig tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in pig you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-09-26
Question
Our lab want to know about to test anti-BMP-3A antibody A04449-1 on rat lung for research purposes, then I may be interested in using anti-BMP-3A antibody A04449-1 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-06-13
Answer
The products we sell, including anti-BMP-3A antibody A04449-1, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-06-13
Question
Is a blocking peptide available for product anti-BMP-3A antibody (A04449-1)?
Verified Customer
Verified customer
Asked: 2019-05-17
Answer
We do provide the blocking peptide for product anti-BMP-3A antibody (A04449-1). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2019-05-17
Question
See attached the WB image, lot number and protocol we used for lung using anti-BMP-3A antibody A04449-1. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2018-07-13
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-07-13
Question
I see that the anti-BMP-3A antibody A04449-1 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2018-07-12
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2018-07-12
Question
Does anti-BMP-3A antibody A04449-1 work on horse IHC with lung?
Verified Customer
Verified customer
Asked: 2018-01-10
Answer
Our lab technicians have not validated anti-BMP-3A antibody A04449-1 on horse. You can run a BLAST between horse and the immunogen sequence of anti-BMP-3A antibody A04449-1 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated horse samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in horse lung in IHC, you can get your next antibody for free.
Boster Scientific Support
Answered: 2018-01-10
Question
Is there a BSA free version of anti-BMP-3A antibody A04449-1 available?
Verified Customer
Verified customer
Asked: 2017-07-21
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-BMP-3A antibody A04449-1 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2017-07-21
Question
Can you help my question with product A04449-1, anti-BMP-3A antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2017-05-31
Answer
We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A04449-1 anti-BMP-3A antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2017-05-31
Question
Does anti-BMP-3A antibody A04449-1 work for WB with lung?
J. Thomas
Verified customer
Asked: 2015-01-09
Answer
According to the expression profile of lung, BMP3 is highly expressed in lung. So, it is likely that anti-BMP-3A antibody A04449-1 will work for WB with lung.
Boster Scientific Support
Answered: 2015-01-09
