Anti-BHC80/PHF21A Antibody Picoband®

PHF21A antibody

Boster Bio Anti-BHC80/PHF21A Antibody catalog # A08507-1. Tested in ELISA, Flow Cytometry, IF, IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Product Info Summary

SKU: A08507-1
Size: 100 μg/vial
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: ELISA, Flow Cytometry, IF, IHC, WB

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Product Name

Anti-BHC80/PHF21A Antibody Picoband®

View all PHF21A Antibodies

SKU/Catalog Number

A08507-1

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-BHC80/PHF21A Antibody catalog # A08507-1. Tested in ELISA, Flow Cytometry, IF, IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-BHC80/PHF21A Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A08507-1)

Host

Rabbit

Contents

Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E.coli-derived human BHC80/PHF21A recombinant protein (Position: M1-Q97).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

A08507-1 is reactive to PHF21A in Human, Mouse, Rat

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Observed Molecular Weight

75-95 kDa

Calculated molecular weight

74.854kDa

Background of PHF21A

PHD finger protein 21A is a protein that in humans is encoded by the PHF21A gene. The PHF21A gene encodes BHC80, a component of a BRAF35 (MIM 605535)/histone deacetylase (HDAC; see MIM 601241) complex (BHC) that mediates repression of neuron-specific genes through the cis-regulatory element known as repressor element-1 (RE1) or neural restrictive silencer (NRS).

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

A08507-1 is guaranteed for ELISA, Flow Cytometry, IF, IHC, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml, Human,Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, By Heat
Immunofluorescence, 2μg/ml, Human
Flow Cytometry (Fixed), 1-3μg/1x106 cells, Human
ELISA, 0.1-0.5μg/ml, -

Positive Control

WB: human A549 whole cell, rat brain tissue, mouse brain tissue,
IHC: human Ovarian cancer tissue, human oesophagus squama cancer tissue, human tonsil tissue, human endometrial carcinoma tissue, human gastric cancer tissue, human intestinal cancer tissue, human lung cancer tissue, human placenta tissue, human sarcoma tissue, human glioma tissue
IF: human oesophagus squama cancer tissue, human intestinal cancer tissue
FCM: HeLa cell, SiHa cell

Validation Images & Assay Conditions

Gene/Protein Information For PHF21A (Source: Uniprot.org, NCBI)

Gene Name

PHF21A

Full Name

PHD finger protein 21A

Weight

74.854kDa

Alternative Names

PHD finger protein 21A; BHC80a; BRAF35-HDAC complex protein BHC80; PHF21A; BHC80; KIAA1696; BM-006 PHF21A BHC80, BM-006, IDDBCS, NEDMS PHD finger protein 21A PHD finger protein 21A|BHC80a|BRAF35-HDAC complex protein BHC80|BRAF35/HDAC2 complex (80 kDa)

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on PHF21A, check out the PHF21A Infographic

PHF21A infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for PHF21A: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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5 Customer Q&As for Anti-BHC80/PHF21A Antibody Picoband®

Question

Would anti-BHC80/PHF21A antibody A08507-1 work for ELISA with cervix carcinoma?

C. Yang

Verified customer

Asked: 2019-12-16

Answer

According to the expression profile of cervix carcinoma, PHF21A is highly expressed in cervix carcinoma. So, it is likely that anti-BHC80/PHF21A antibody A08507-1 will work for ELISA with cervix carcinoma.

Boster Scientific Support

Answered: 2019-12-16

Question

I see that the anti-BHC80/PHF21A antibody A08507-1 works with ELISA, what is the protocol used to produce the result images on the product page?

Verified Customer

Verified customer

Asked: 2019-07-19

Answer

You can find protocols for ELISA on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2019-07-19

Question

Is this A08507-1 anti-BHC80/PHF21A antibody reactive to the isotypes of PHF21A?

Verified Customer

Verified customer

Asked: 2018-06-12

Answer

The immunogen of A08507-1 anti-BHC80/PHF21A antibody is E.coli-derived human BHC80/PHF21A recombinant protein (Position: M1-Q97). Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?

Boster Scientific Support

Answered: 2018-06-12

Question

Do you have a BSA free version of anti-BHC80/PHF21A antibody A08507-1 available?

M. Johnson

Verified customer

Asked: 2016-04-27

Answer

Thank you for your recent telephone inquiry. I can confirm that some lots of this anti-BHC80/PHF21A antibody A08507-1 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.

Boster Scientific Support

Answered: 2016-04-27

Question

We are currently using anti-BHC80/PHF21A antibody A08507-1 for human tissue, and we are happy with the ELISA results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on zebrafish tissues as well?

M. Jha

Verified customer

Asked: 2014-03-07

Answer

The anti-BHC80/PHF21A antibody (A08507-1) has not been validated for cross reactivity specifically with zebrafish tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in zebrafish you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2014-03-07

Order DetailsPrice
A08507-1

100μg

$370
A08507-1-10ug

10μg sample (liquid)

$99
A08507-1-Biotin

100 μg Biotin conjugated

$570
A08507-1-Cy3

100 μg Cy3 conjugated

$570
A08507-1-Dylight488

100 μg Dylight488 conjugated

$570
A08507-1-Dylight550

100 μg Dylight550 conjugated

$570
A08507-1-Dylight594

100 μg Dylight594 conjugated

$570
A08507-1-FITC

100 μg FITC conjugated

$570
A08507-1-HRP

100 μg HRP conjugated

$570
A08507-1-APC

100 μg APC conjugated

$670
A08507-1-PE

100 μg PE conjugated

$670
A08507-1-iFluor647

100 μg iFluor647 conjugated

$670
A08507-1-carrier-free

Carrier Free

$370

More conjugates/formats

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A08507-1
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00

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