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Product Info Summary
| SKU: | M05613-5 |
|---|---|
| Size: | 100 μl/vial |
| Reactive Species: | Chicken, Hamster, Human, Mouse, Rat |
| Host: | Mouse |
| Application: | IF, IHC, ICC, WB |
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Product info
Product Name
Anti-beta Tubulin Mouse Monoclonal Antibody
View all beta Tubulin Antibodies
SKU/Catalog Number
M05613-5
Size
100 μl/vial
Form
Liquid
Description
Boster Bio Anti-beta Tubulin Mouse Monoclonal Antibody catalog # M05613-5. Tested in WB, IHC, ICC/IF applications. This antibody reacts with Human, Mouse, Rat, Chicken, Hamster.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-beta Tubulin Mouse Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M05613-5)
Host
Mouse
Contents
Mouse IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
17T13
Isotype
IgG1
Immunogen
A synthesized peptide derived from human beta Tubulin
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M05613-5 is reactive to TUBB in Chicken, Hamster, Human, Mouse, Rat
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
50 kDa
Calculated molecular weight
49671 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M05613-5 is guaranteed for IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:3000-1:10000
IHC 1:50-1:200
ICC/IF 1:50-1:200
Positive Control
WB: human Hela whole cell, human K562 whole cell, human MCF-7 whole cell, human A549 whole cell, rat brain tissue, rat kidney tissue, mouse brain tissue, mouse kidney tissue, A431 cell, Rat brain, Mouse kidney, Chicken lung, Hamster spleen
IHC: human breast carcinoma tissue
ICC/IF: Hela cell
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of beta Tubulin using anti-beta Tubulin antibody (M05613-5).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human K562 whole cell lysates,
Lane 3: human MCF-7 whole cell lysates,
Lane 4: human A549 whole cell lysates,
Lane 5: rat brain tissue lysates,
Lane 6: rat kidney tissue lysates,
Lane 7: mouse brain tissue lysates,
Lane 8: mouse kidney tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-beta Tubulin antigen affinity purified monoclonal antibody (Catalog # M05613-5) at 1:3000 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for beta Tubulin at approximately 50 kDa. The expected band size for beta Tubulin is at 50 kDa.
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Western blot analysis of beta Actin expression in (1) A431 cell lysate; (2) Rat brain lysate; (3) Mouse kidney lysate; (4) Chicken lung lysate; (5) Hamster spleen lysate with beta Tubulin Mouse Monoclonal Antibody.
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Immunohistochemical analysis of paraffin-embedded human breast carcinoma, using beta Tubulin Mouse Monoclonal Antibody.
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Immunofluorescent analysis of Hela cells, using beta Tubulin mAb.
Protein Target Info & Infographic
Gene/Protein Information For TUBB (Source: Uniprot.org, NCBI)
Gene Name
TUBB
Full Name
Tubulin beta chain
Weight
49671 MW
Superfamily
tubulin family
Alternative Names
Tubulin beta chain;Tubulin beta-5 chain;TUBB;TUBB5;OK/SW-cl.56; TUBB CDCBM6, CSCSC1, M40, OK/SW-cl.561, TUBB5, TUBB tubulin beta class I tubulin beta chain|beta Ib tubulin|epididymis secretory sperm binding protein|tubulin beta-1 chain|tubulin beta-5 chain|tubulin, beta polypeptide
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on TUBB, check out the TUBB Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for TUBB: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-beta Tubulin Mouse Monoclonal Antibody (M05613-5)
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