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Product Info Summary
| SKU: | M01857 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IP, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-beta III Tubulin TUBB3 Rabbit Monoclonal Antibody
View all beta-III Tubulin Antibodies
SKU/Catalog Number
M01857
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-beta III Tubulin TUBB3 Rabbit Monoclonal Antibody catalog # M01857. Tested in WB, IHC, ICC/IF, IP applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-beta III Tubulin TUBB3 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01857)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
CB-20
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human beta III Tubulin
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M01857 is reactive to TUBB3 in Human, Mouse, Rat
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
53 kDa
Calculated molecular weight
50433 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M01857 is guaranteed for IP, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:2000-1:20000
IHC 1:50-1:200
ICC/IF 1:50-1:200
IP 1:50
Positive Control
WB: HeLa cell
IHC: human ovarian cancer tissue
Validation Images & Assay Conditions
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Immunohistochemical analysis of paraffin-embedded human ovarian cancer, using beta III Tubulin Antibody(M01857)
TUBB3 was detected in paraffin-embedded tissue section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-TUBB3 Antibody (M01857)overnight at 4 Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
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Western blot analysis of beta III Tubulin expression in HeLa cell lysate (M01857).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TUBB3 monoclonal antibody (Catalog # M01857) overnight at 4 then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TUBB3
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AB0042Immunofluorescent analysis of PC12 cells, using beta Tubulin Antibody .
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Immunofluorescent analysis using the Antibody at 1:50 dilution.
Protein Target Info & Infographic
Gene/Protein Information For TUBB3 (Source: Uniprot.org, NCBI)
Gene Name
TUBB3
Full Name
Tubulin beta-3 chain
Weight
50433 MW
Superfamily
tubulin family
Alternative Names
Tubulin beta-3 chain;Tubulin beta-4 chain;Tubulin beta-III;TUBB3;TUBB4; TUBB3 CDCBM, CDCBM1, CFEOM3, CFEOM3A, FEOM3, TUBB4, beta-4 tubulin beta 3 class III tubulin beta-3 chain|class III beta-tubulin|tubulin beta-4 chain|tubulin beta-III|tubulin, beta 3
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on TUBB3, check out the TUBB3 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for TUBB3: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-beta III Tubulin TUBB3 Rabbit Monoclonal Antibody (M01857)
Hello CJ!
M01857 has been cited in 1 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Mesenchymal Stem Cells Could Be Considered as a Candidate for Further Studies in Cell-Based Therapy of Alzheimer’s Disease via Targeting the Signaling Pathways
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Customer Q&As
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1 Customer Q&As for Anti-beta III Tubulin TUBB3 Rabbit Monoclonal Antibody
Question
Is M01857 suitable for mouse tissue immunostaining?
Verified customer
Asked: 2019-10-09
Answer
Yes, the Anti-Beta III Tubulin TUBB3 Rabbit Monoclonal Antibody (M01857) is suitable for mouse tissue immunostaining.
Boster Scientific Support
Answered: 2019-10-09
