Product Info Summary
SKU: | M00035-2 |
---|---|
Size: | 100 μl/vial |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | IF, IHC, ICC, WB |
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Product info
Product Name
Anti-BDNF Rabbit Monoclonal Antibody
SKU/Catalog Number
M00035-2
Size
100 μl/vial
Form
Liquid
Description
Boster Bio Anti-BDNF Rabbit Monoclonal Antibody catalog # M00035-2. Tested in WB, IHC, ICC/IF applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-BDNF Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00035-2)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
17B21
Isotype
IgG
Immunogen
A synthesized peptide derived from human BDNF
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M00035-2 is reactive to BDNF in Human, Mouse, Rat
Applications
M00035-2 is guaranteed for IF, IHC, ICC, WB Boster Guarantee
Observed Molecular Weight
110 kDa
Calculated molecular weight
27818 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Assay dilution & Images
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:1000-1:2000
IHC 1:50-1:200
ICC/IF 1:50-1:200
Validation Images & Assay Conditions
![m00035 2 wb7 m00035 2 wb7](https://www.bosterbio.com/media/catalog/product/m/0/m00035-2-wb7.jpg)
Click image to see more details
All lanes use the Antibody at 1:1W dilution for 1 hour at room temperature.
![m00035 2 wb m00035 2 wb](https://www.bosterbio.com/media/catalog/product/m/0/m00035-2-wb.jpg)
Click image to see more details
Western blot analysis of extracts of Mouse heart lysate, using BDNF antibody
![m00035 2 ihc9 m00035 2 ihc9](https://www.bosterbio.com/media/catalog/product/m/0/m00035-2-ihc9.jpg)
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Immunohistochemical analysis of paraffin-embedded Human breast cancer, using the Antibody at 1:1500 dilution.
![m00035 2 ihc7 m00035 2 ihc7](https://www.bosterbio.com/media/catalog/product/m/0/m00035-2-ihc7.jpg)
Click image to see more details
Immunohistochemical analysis of paraffin-embedded Human Hodgkin's lymphoma, using the Antibody at 1:1500 dilution.
![m00035 2 ihc8 m00035 2 ihc8](https://www.bosterbio.com/media/catalog/product/m/0/m00035-2-ihc8.jpg)
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Immunohistochemical analysis of paraffin-embedded Human prostate cancer, using the Antibody at 1:1500 dilution.
![m00035 2 ihc m00035 2 ihc](https://www.bosterbio.com/media/catalog/product/m/0/m00035-2-ihc.jpg)
Click image to see more details
Immunohistochemical analysis of paraffin-embedded human brain, using BDNF Antibody.
![m00035 2 icc1 m00035 2 icc1](https://www.bosterbio.com/media/catalog/product/m/0/m00035-2-icc1.jpg)
Click image to see more details
Immunofluorescent analysis using the Antibody at 1:150 dilution.
![m00035 2 icc2 m00035 2 icc2](https://www.bosterbio.com/media/catalog/product/m/0/m00035-2-icc2.jpg)
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Immunofluorescent analysis using the Antibody at 1:500 dilution.
![m00035 2 if m00035 2 if](https://www.bosterbio.com/media/catalog/product/m/0/m00035-2-if.jpg)
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Immunofluorescent analysis of Hela cells, using BDNF Antibody.
Protein Target Info & Infographic
Gene/Protein Information For BDNF (Source: Uniprot.org, NCBI)
Gene Name
BDNF
Full Name
Brain-derived neurotrophic factor
Weight
27818 MW
Superfamily
NGF-beta family
Alternative Names
Abrineurin; ANON2; BDNF; brain-derived neurotrophic factor; BULN2; MGC34632; Neurotrophin BDNF ANON2, BULN2 brain derived neurotrophic factor brain-derived neurotrophic factor|abrineurin|neurotrophin
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on BDNF, check out the BDNF Infographic
![BDNF infographic](/media/images/gene-infographic-example.jpg)
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for BDNF: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-BDNF Rabbit Monoclonal Antibody (M00035-2)
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