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Product Info Summary
| SKU: | M00035-1 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IF, IHC, ICC, WB |
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Product info
Product Name
Anti-BDNF Rabbit Monoclonal Antibody
SKU/Catalog Number
M00035-1
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-BDNF Rabbit Monoclonal Antibody catalog # M00035-1. Tested in WB, IHC, ICC/IF applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-BDNF Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00035-1)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
BEH-2
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human BDNF
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M00035-1 is reactive to BDNF in Human, Mouse, Rat
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
110 kDa
Calculated molecular weight
27818 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00035-1 is guaranteed for IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:50-1:200
ICC/IF 1:50-1:200
Positive Control
IHC: Human esophageal carcinoma tissue, Human lung tissue, Rat stomach tissue, Mouse heart tissue, Rat pancreas tissue, human testis tissue
Validation Images & Assay Conditions
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All lanes use the Antibody at 1:5k dilution for 1 hour at room temperature.
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Western blot analysis of extracts of Human cerebellum lysate, using BDNF antibody.
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Immunohistochemical analysis of paraffin-embedded Human esophageal carcinoma, using the Antibody at 1:50 dilution.
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Immunohistochemical analysis of paraffin-embedded Human lung, using the Antibody at 1:50 dilution.
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Immunohistochemical analysis of paraffin-embedded Rat stomach, using the Antibody at 1:50 dilution.
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Immunohistochemical analysis of paraffin-embedded Mouse heart, using the Antibody at 1:50 dilution.
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Immunohistochemical analysis of paraffin-embedded Rat pancreas, using the Antibody at 1:50 dilution.
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Immunohistochemical analysis of paraffin-embedded human testis, using BDNF Antibody .
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Immunofluorescent analysis using the Antibody at 1:500 dilution.
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Immunofluorescent analysis of HeLa cells, using BDNF Antibody.
Protein Target Info & Infographic
Gene/Protein Information For BDNF (Source: Uniprot.org, NCBI)
Gene Name
BDNF
Full Name
Brain-derived neurotrophic factor
Weight
27818 MW
Superfamily
NGF-beta family
Alternative Names
Brain-derived neurotrophic factor;BDNF;Abrineurin;BDNF; BDNF ANON2, BULN2 brain derived neurotrophic factor brain-derived neurotrophic factor|abrineurin|neurotrophin
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on BDNF, check out the BDNF Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for BDNF: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-BDNF Rabbit Monoclonal Antibody (M00035-1)
Hello CJ!
M00035-1 has been cited in 12 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Xue LL,Du RL,Hu Y,Xiong LL,Su ZY,Ma Z,Tan YX,Liu J,Hu Q,Zhu ZQ,Wang TH,Liu XZ.BDNF promotes neuronal survival after neonatal hypoxic-ischemic encephalopathy by up-regulating Stx1b and suppressing VDAC1.Brain Res Bull.2021 May 28:S0361-9230(21)00144-1.doi:10.1016/j.brainresbull.2021.05.013.Epub ahead of print.PMID:34058286.
Species: Rat
M00035-1 usage in article: APP:WB, SAMPLE:CORTEX AND HIPPOCAMPUS, DILUTION:1:100
Huangqin flavonoid extraction for spinal cord injury in a rat model
Serotonin regulates brain-derived neurotrophic factor expression in select brain regions during acute psychological stress
Beneficial effect of fluoxetine treatment aganist psychological stress is mediated by increasing BDNF expression in selected brain areas
Brain-derived neurotrophic factor is up-regulated in severe acute cauda equina syndrome dog model
Changes in compressed neurons from dogs with acute and severe cauda equina constrictions following intrathecal injection of brain-derived neurotrophic factor-conjugated polymer nanoparticles
Acute Stress and Chronic Stress Change Brain-Derived Neurotrophic Factor (BDNF) and Tyrosine Kinase-Coupled Receptor (TrkB) Expression in Both Young and Aged Rat Hippocampus
Electroacupuncture Ameliorates Learning and Memory and Improves Synaptic Plasticity via Activation of the PKA/CREB Signaling Pathway in Cerebral Hypoperfusion
Fingolimod induces neurogenesis in adult mouse hippocampus and improves contextual fear memory
Transplantation of human umbilical cord blood mesenchymal stem cells to treat a rat model of traumatic brain injury
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