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Product Info Summary
| SKU: | M00040-1 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | Flow Cytometry, IP, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-Bcl-2 Rabbit Monoclonal Antibody
SKU/Catalog Number
M00040-1
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-Bcl-2 Rabbit Monoclonal Antibody catalog # M00040-1. Tested in WB, IHC, ICC/IF, IP, Flow Cytometry applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Bcl-2 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00040-1)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
HF-2
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human Bcl-2
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M00040-1 is reactive to BCL2 in Human
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
83, 88 kDa
Calculated molecular weight
26266 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00040-1 is guaranteed for Flow Cytometry, IP, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:50-1:100
ICC/IF 1:50-1:100
IP 1:50
FC 1:50
Positive Control
IHC: Human non-Hodgkin's lymphoma tissue, Human squamous cell carcinoma tissue, Rat liver tissue, Mouse skin tissue, Rat stomach tissue, human spleen tissue
ICC/IF: Jurkat cell
Validation Images & Assay Conditions
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Western blot analysis of Bcl-2 expression in (1) Jurkat cell lysate;(2) MCF-7 cell lysate.
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All lanes use the Antibody at 1:1W dilution for 1 hour at room temperature.
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Immunohistochemical analysis of paraffin-embedded Human non-Hodgkin's lymphoma, using the Antibody at 1:50 dilution.
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Immunohistochemical analysis of paraffin-embedded Human squamous cell carcinoma , using the Antibody at 1:50 dilution.
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Immunohistochemical analysis of paraffin-embedded Rat liver, using the Antibody at 1:50 dilution.
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Immunohistochemical analysis of paraffin-embedded Mouse skin, using the Antibody at 1:50 dilution.
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Immunohistochemical analysis of paraffin-embedded Rat stomach, using the Antibody at 1:50 dilution.
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Immunohistochemical analysis of paraffin-embedded human spleen, using Bcl-2 Antibody.
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Immunofluorescent analysis of Jurkat cells, using Bcl-2 Antibody .
Protein Target Info & Infographic
Gene/Protein Information For BCL2 (Source: Uniprot.org, NCBI)
Gene Name
BCL2
Full Name
Apoptosis regulator Bcl-2
Weight
26266 MW
Superfamily
Bcl-2 family
Alternative Names
Apoptosis regulator Bcl-2;BCL2; BCL2 Bcl-2, PPP1R50 BCL2 apoptosis regulator apoptosis regulator Bcl-2|B-cell CLL/lymphoma 2|protein phosphatase 1, regulatory subunit 50
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on BCL2, check out the BCL2 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for BCL2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Bcl-2 Rabbit Monoclonal Antibody (M00040-1)
Hello CJ!
M00040-1 has been cited in 40 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Anti-tumor effect and mechanistic study of elemene on pancreatic carcinoma Jin Long,1 Zhe Liu,1 and Lian Hui2 BMC Complement Altern Med. 2019; 19: 133. Published online 2019 Jun 18. doi: 10.1186/s12906-019-2544-2
SIRT1 protects against apoptosis by promoting autophagy in the oxygen glucose deprivation/reperfusion-induced injury Qiannan Ren, et al. Front Neurol. 2019 Dec 5;10:1289. doi: 10.3389/fneur.2019.01289. eCollection 2019.
Oleanolic acid from Prunella Vulgaris L. Induces SPC-A-1 cell line apoptosis via regulation of Bax, Bad and Bcl-2 Expression
Baicalin attenuates alcoholic liver injury through modulation of hepatic oxidative stress, inflammation and sonic hedgehog pathway in rats
Antitumor effects of Laminaria extract fucoxanthin on lung cancer
Recombinant Newcastle disease virus expressing human IFN%u2010%u03BB1 (rL%u2010hIFN%u2010%u03BB1)%u2010induced apoptosis of A549 cells is connected to endoplasmic reticulum stress %u2026
The inhibition effect of triptolide on human endometrial carcinoma cell line HEC-1B: A in vitro and in vivo studies
Ibutilide treatment protects against ER stress induced apoptosis by regulating calumenin expression in tunicamycin treated cardiomyocytes
NOB1 silencing inhibits the growth and metastasis of laryngeal cancer cells through the regulation of JNK signaling pathway
Transforming growth factor-beta1 suppresses hepatocellular carcinoma proliferation via activation of Hippo signaling
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Customer Reviews
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Customer Q&As
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16 Customer Q&As for Anti-Bcl-2 Rabbit Monoclonal Antibody
Question
We are currently using anti-Bcl-2 Rabbit Monoclonal antibody M00040-1 for human tissue, and we are content with the IF results. The species of reactivity given in the datasheet says human. Is it true that the antibody can work on primate tissues as well?
Verified Customer
Verified customer
Asked: 2020-04-27
Answer
The anti-Bcl-2 Rabbit Monoclonal antibody (M00040-1) has not been tested for cross reactivity specifically with primate tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in primate you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-04-27
Question
We need to test anti-Bcl-2 Rabbit Monoclonal antibody M00040-1 on human thyroid gland for research purposes, then I may be interested in using anti-Bcl-2 Rabbit Monoclonal antibody M00040-1 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2020-03-17
Answer
The products we sell, including anti-Bcl-2 Rabbit Monoclonal antibody M00040-1, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2020-03-17
Question
I see that the anti-Bcl-2 Rabbit Monoclonal antibody M00040-1 works with ICC, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-12-23
Answer
You can find protocols for ICC on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2019-12-23
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for thyroid gland using anti-Bcl-2 Rabbit Monoclonal antibody M00040-1. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-11-01
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-11-01
Question
Will M00040-1 anti-Bcl-2 Rabbit Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-10-30
Answer
As indicated on the product datasheet, M00040-1 anti-Bcl-2 Rabbit Monoclonal antibody as been tested on ICC. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-10-30
Question
My question regarding product M00040-1, anti-Bcl-2 Rabbit Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
G. Li
Verified customer
Asked: 2019-10-15
Answer
It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M00040-1 anti-Bcl-2 Rabbit Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-10-15
Question
We have tried in the past anti-Bcl-2 Rabbit Monoclonal antibody for IP on testis last year. I am using human, and I plan to use the antibody for ICC next. We need examining testis as well as thyroid gland in our next experiment. Do you have any suggestion on which antibody would work the best for ICC?
Verified Customer
Verified customer
Asked: 2019-10-14
Answer
I took a look at the website and datasheets of our anti-Bcl-2 Rabbit Monoclonal antibody and it seems that M00040-1 has been tested on human in both IP and ICC. Thus M00040-1 should work for your application. Our Boster satisfaction guarantee will cover this product for ICC in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for ICC detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2019-10-14
Question
We have observed staining in human thyroid gland. Any tips? Is anti-Bcl-2 Rabbit Monoclonal antibody supposed to stain thyroid gland positively?
Verified Customer
Verified customer
Asked: 2019-09-04
Answer
From what I have seen in literature thyroid gland does express BCL2. From what I have seen in Uniprot.org, BCL2 is expressed in thyroid gland, testis, among other tissues. Regarding which tissues have BCL2 expression, here are a few articles citing expression in various tissues:
Testis, Pubmed ID: 15489334
Boster Scientific Support
Answered: 2019-09-04
Question
I was wanting to use your anti-Bcl-2 Rabbit Monoclonal antibody for ICC for human thyroid gland on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human thyroid gland identification?
Verified Customer
Verified customer
Asked: 2019-08-20
Answer
You can see on the product datasheet, M00040-1 anti-Bcl-2 Rabbit Monoclonal antibody has been tested for Flow Cytometry, IP, IF, IHC, ICC, WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human thyroid gland in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-08-20
Question
I have attached the WB image, lot number and protocol we used for thyroid gland using anti-Bcl-2 Rabbit Monoclonal antibody M00040-1. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-08-16
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-08-16
Question
Will anti-Bcl-2 Rabbit Monoclonal antibody M00040-1 work for ICC with thyroid gland?
S. Jones
Verified customer
Asked: 2019-06-26
Answer
According to the expression profile of thyroid gland, BCL2 is highly expressed in thyroid gland. So, it is likely that anti-Bcl-2 Rabbit Monoclonal antibody M00040-1 will work for ICC with thyroid gland.
Boster Scientific Support
Answered: 2019-06-26
Question
Is there a BSA free version of anti-Bcl-2 Rabbit Monoclonal antibody M00040-1 available?
Verified Customer
Verified customer
Asked: 2019-03-26
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-Bcl-2 Rabbit Monoclonal antibody M00040-1 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-03-26
Question
Is this M00040-1 anti-Bcl-2 Rabbit Monoclonal antibody reactive to the isotypes of BCL2?
Verified Customer
Verified customer
Asked: 2018-07-12
Answer
The immunogen of M00040-1 anti-Bcl-2 Rabbit Monoclonal antibody is A synthesized peptide derived from human Bcl-2. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2018-07-12
Question
My team were well pleased with the WB result of your anti-Bcl-2 Rabbit Monoclonal antibody. However we have seen positive staining in testis mitochondrion outer membrane using this antibody. Is that expected? Could you tell me where is BCL2 supposed to be expressed?
N. Johnson
Verified customer
Asked: 2014-05-02
Answer
From what I have seen in literature, testis does express BCL2. Generally BCL2 expresses in mitochondrion outer membrane. Regarding which tissues have BCL2 expression, here are a few articles citing expression in various tissues:
Testis, Pubmed ID: 15489334
Boster Scientific Support
Answered: 2014-05-02
Question
Is a blocking peptide available for product anti-Bcl-2 Rabbit Monoclonal antibody (M00040-1)?
P. Williams
Verified customer
Asked: 2013-08-06
Answer
We do provide the blocking peptide for product anti-Bcl-2 Rabbit Monoclonal antibody (M00040-1). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2013-08-06
Question
My question regards using your anti-Bcl-2 Rabbit Monoclonal antibody for actin filament organization studies. Has this antibody been tested with western blotting on jurkat cell lysate? We would like to see some validation images before ordering.
J. Wu
Verified customer
Asked: 2013-05-31
Answer
I appreciate your inquiry. This M00040-1 anti-Bcl-2 Rabbit Monoclonal antibody is validated on jurkat cell lysate. It is guaranteed to work for Flow Cytometry, IP, IF, IHC, ICC, WB in human. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2013-05-31
