Click image to see more details
Product Info Summary
| SKU: | A00040-2 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | ELISA, Flow Cytometry, IF, ICC, WB |
Customers Who Bought This Also Bought
Product info
Product Name
Anti-Bcl-2/BCL2 Antibody Picoband®
SKU/Catalog Number
A00040-2
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Bcl-2/BCL2 Antibody Picoband® catalog # A00040-2. Tested in ELISA, Flow Cytometry, IF, ICC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Bcl-2/BCL2 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00040-2)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E. coli-derived human Bcl-2 recombinant protein (Position: Q118-E165).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A00040-2 is reactive to BCL2 in Human
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
26 kDa
Calculated molecular weight
87335 MW
Background of Bcl-2
Immunoreactive BCL2 protein in the neoplastic cells of almost all follicular lymphomas whereas no BCL2 protein was detected in follicles affected by nonneoplastic processes or in normal lymphoid tissue. Every tumor with molecular-genetic evidence of t (14;18) translocation expressed detectable levels of BCL2 protein, regardless of whether the breakpoint was located in or at a distance from the BCL2 gene. Overexpression of BCL2 blocks the apoptotic death of a pro-B-lymphocyte cell line.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A00040-2 is guaranteed for ELISA, Flow Cytometry, IF, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human
Immunocytochemistry/Immunofluorescence, 5 μg/ml, Human
Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human
Positive Control
WB: human Jurkat whole cell, human HL-60 whole cell, human THP-1 whole cell
ICC/IF: U20S cell
FCM: U20S cell
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of BCL2 using anti-BCL2 antibody (A00040-2).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Jurkat whole cell lysates,
Lane 2: human HL-60 whole cell lysates,
Lane 3: human THP-1 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-BCL2 antigen affinity purified polyclonal antibody (Catalog # A00040-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for BCL2 at approximately 26 kDa. The expected band size for BCL2 is at 26 kDa.
Click image to see more details
Figure 3. Flow Cytometry analysis of U20S cells using anti-BCL2 antibody (A00040-2).
Overlay histogram showing U20S cells stained with A00040-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-BCL2 Antibody (A00040-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Click image to see more details
Figure 2. IF analysis of BCL2 and Tubulin alpha using anti-BCL2 antibody (A00040-2) and anti-Tubulin alpha antibody (M03989-3).
BCL2 and Tubulin alpha were detected in immunocytochemical section of U20S cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5μg/mL rabbit anti-BCL2 antibody (A00040-2) and mouse anti-Tubulin alpha Antibody (M03989-3) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) and DyLight®488 Conjugated Goat Anti-Mouse IgG (BA1126) were used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
Protein Target Info & Infographic
Gene/Protein Information For BCL2 (Source: Uniprot.org, NCBI)
Gene Name
BCL2
Full Name
Apoptosis regulator Bcl-2
Weight
87335 MW
Superfamily
Bcl-2 family
Alternative Names
Apoptosis regulator Bcl-2; BCL2 BCL2 Bcl-2, PPP1R50 BCL2 apoptosis regulator apoptosis regulator Bcl-2|B-cell CLL/lymphoma 2|protein phosphatase 1, regulatory subunit 50
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on BCL2, check out the BCL2 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for BCL2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Bcl-2/BCL2 Antibody Picoband® (A00040-2)
Hello CJ!
A00040-2 has been cited in 240 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Inhibition of glioblastoma growth and invasion by 125I brachytherapy in rat glioma model
Lipoxin A4 pretreatment mitigates skeletal muscle ischemia-reperfusion injury in rats
The Effects of Fasudil at Different Doses on Acute Myocardial Infarction in Rats
Effect of curcumin on Bcl-2 and Bax expression in nude mice prostate cancer
Effects of intracerebral hemorrhage and subsequent minimally invasive hematoma aspiration on expression of apoptosisrelated genes in rats
Effects of PDTC on NF-κB expression and apoptosis in rats with severe acute pancreatitis-associated lung injury
Alpha-lipoic acid attenuates trinitrobenzene sulfonic acid-induced ulcerative colitis in mice
A Study on Expression and Tyrosine 705 phosphorylation of STAT3 in Focal Cerebral Ischemia-Reperfusion Rat Model and its Role in Neuronal Apoptosis
Radix Ilicis Pubescentis total flavonoids combined with mobilization of bone marrow stem cells to protect against cerebral ischemia/reperfusion injury
Cycloartan-24-ene-1α,2α,3β-triol, a cycloartane-type triterpenoid from the resinous exudates of Commiphora myrrha, induces apoptosis in human prostatic cancer PC-3 cells
Recommended Resources
Here are featured tools and databases that you might find useful.
- Boster's Pathways Library
- Protein Databases
- Bioscience Research Protocol Resources
- Data Processing & Analysis Software
- Photo Editing Software
- Scientific Literature Resources
- Research Paper Management Tools
- Molecular Biology Software
- Primer Design Tools
- Bioinformatics Tools
- Phylogenetic Tree Analysis
Customer Reviews
Have you used Anti-Bcl-2/BCL2 Antibody Picoband®?
Submit a review and receive an Amazon gift card.
- $30 for a review with an image
0 Reviews For Anti-Bcl-2/BCL2 Antibody Picoband®
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question
16 Customer Q&As for Anti-Bcl-2/BCL2 Antibody Picoband®
Question
Is a blocking peptide available for product anti-Bcl-2/BCL2 antibody (A00040-2)?
Verified Customer
Verified customer
Asked: 2020-04-21
Answer
We do provide the blocking peptide for product anti-Bcl-2/BCL2 antibody (A00040-2). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2020-04-21
Question
We have tried in the past anti-Bcl-2/BCL2 antibody for IHC on testis in a previous project. I am using rat, and We intend to use the antibody for ELISA next. My question regards examining testis as well as thyroid gland in our next experiment. Do you have any suggestion on which antibody would work the best for ELISA?
Verified Customer
Verified customer
Asked: 2019-12-19
Answer
I viewed the website and datasheets of our anti-Bcl-2/BCL2 antibody and it appears that A00040-2 has been validated on rat in both IHC and ELISA. Thus A00040-2 should work for your application. Our Boster satisfaction guarantee will cover this product for ELISA in rat even if the specific tissue type has not been validated. We do have a comprehensive range of products for ELISA detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2019-12-19
Question
We were well pleased with the WB result of your anti-Bcl-2/BCL2 antibody. However we have observed positive staining in testis mitochondrion outer membrane using this antibody. Is that expected? Could you tell me where is BCL2 supposed to be expressed?
Verified Customer
Verified customer
Asked: 2019-12-19
Answer
Based on literature, testis does express BCL2. Generally BCL2 expresses in mitochondrion outer membrane. Regarding which tissues have BCL2 expression, here are a few articles citing expression in various tissues:
Testis, Pubmed ID: 15489334
Boster Scientific Support
Answered: 2019-12-19
Question
I am interested in to test anti-Bcl-2/BCL2 antibody A00040-2 on rat thyroid gland for research purposes, then I may be interested in using anti-Bcl-2/BCL2 antibody A00040-2 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-10-15
Answer
The products we sell, including anti-Bcl-2/BCL2 antibody A00040-2, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-10-15
Question
I was wanting to use your anti-Bcl-2/BCL2 antibody for ELISA for rat thyroid gland on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for rat thyroid gland identification?
Verified Customer
Verified customer
Asked: 2019-08-06
Answer
You can see on the product datasheet, A00040-2 anti-Bcl-2/BCL2 antibody has been validated for ELISA, IHC, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in rat thyroid gland in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-08-06
Question
We are currently using anti-Bcl-2/BCL2 antibody A00040-2 for rat tissue, and we are happy with the ELISA results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on primate tissues as well?
Verified Customer
Verified customer
Asked: 2019-07-03
Answer
The anti-Bcl-2/BCL2 antibody (A00040-2) has not been tested for cross reactivity specifically with primate tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in primate you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-07-03
Question
I am interested in using your anti-Bcl-2/BCL2 antibody for post-embryonic development studies. Has this antibody been tested with western blotting on small intestine tissue? We would like to see some validation images before ordering.
Verified Customer
Verified customer
Asked: 2019-06-28
Answer
Thanks for your inquiry. This A00040-2 anti-Bcl-2/BCL2 antibody is validated on small intestine tissue. It is guaranteed to work for ELISA, IHC, WB in human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2019-06-28
Question
I have a question about product A00040-2, anti-Bcl-2/BCL2 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
G. Carter
Verified customer
Asked: 2019-04-22
Answer
We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A00040-2 anti-Bcl-2/BCL2 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-04-22
Question
Is there a BSA free version of anti-Bcl-2/BCL2 antibody A00040-2 available?
Verified Customer
Verified customer
Asked: 2018-05-16
Answer
Thank you for your recent telephone inquiry. I can confirm that some lots of this anti-Bcl-2/BCL2 antibody A00040-2 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2018-05-16
Question
Will anti-Bcl-2/BCL2 antibody A00040-2 work for ELISA with thyroid gland?
Verified Customer
Verified customer
Asked: 2017-08-01
Answer
According to the expression profile of thyroid gland, BCL2 is highly expressed in thyroid gland. So, it is likely that anti-Bcl-2/BCL2 antibody A00040-2 will work for ELISA with thyroid gland.
Boster Scientific Support
Answered: 2017-08-01
Question
Thanks for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for thyroid gland using anti-Bcl-2/BCL2 antibody A00040-2. Let me know if you need anything else.
L. Williams
Verified customer
Asked: 2016-05-10
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2016-05-10
Question
I see that the anti-Bcl-2/BCL2 antibody A00040-2 works with ELISA, what is the protocol used to produce the result images on the product page?
Z. Jackson
Verified customer
Asked: 2016-01-19
Answer
You can find protocols for ELISA on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2016-01-19
Question
Is this A00040-2 anti-Bcl-2/BCL2 antibody reactive to the isotypes of BCL2?
N. Dhar
Verified customer
Asked: 2015-09-28
Answer
The immunogen of A00040-2 anti-Bcl-2/BCL2 antibody is E. coli-derived human Bcl-2 recombinant protein (Position: Q118-E165). Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2015-09-28
Question
I have attached the WB image, lot number and protocol we used for thyroid gland using anti-Bcl-2/BCL2 antibody A00040-2. Please let me know if you require anything else.
G. Banerjee
Verified customer
Asked: 2015-05-05
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2015-05-05
Question
Does A00040-2 anti-Bcl-2/BCL2 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
M. Jha
Verified customer
Asked: 2014-03-07
Answer
As indicated on the product datasheet, A00040-2 anti-Bcl-2/BCL2 antibody as been tested on ELISA. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2014-03-07
Question
We have observed staining in mouse testis. Are there any suggestions? Is anti-Bcl-2/BCL2 antibody supposed to stain testis positively?
S. Collins
Verified customer
Asked: 2013-06-14
Answer
Based on literature testis does express BCL2. Based on Uniprot.org, BCL2 is expressed in thyroid gland, testis, among other tissues. Regarding which tissues have BCL2 expression, here are a few articles citing expression in various tissues:
Testis, Pubmed ID: 15489334
Boster Scientific Support
Answered: 2013-06-14
