Anti-Batf2 Antibody Picoband™

Product Name

Anti-Batf2 Antibody Picoband™

View all MGC20410 Antibodies

SKU/Catalog Number

A09891-2

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-Batf2 Antibody Picoband™ catalog # A09891-2. Tested in ELISA, IHC, WB applications. This antibody reacts with Mouse, Rat.

Storage & Handling

At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Cite This Product

Anti-Batf2 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A09891-2)

Host

Rabbit

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E.coli-derived mouse Batf2 recombinant protein (Position: M1-L138).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

A09891-2 is reactive to Batf2 in Mouse, Rat

Applications

A09891-2 is guaranteed for ELISA, IHC, WB Boster Guarantee

Observed Molecular Weight

25 kDa

Calculated molecular weight

Background of MGC20410

Basic leucine zipper transcription factor, ATF-like 2 is a protein that, in humans, is encoded by the BATF2 gene. BATF2 (basic leucine zipper transcription factor, ATF-like 2) is a 274 amino acid protein that localizes to the nucleus and contains one bZIP domain, suggesting that it may be involved in transcriptional regulation. The gene encoding BATF2, which is expressed as multiple alternatively spliced isoforms, is located on human chromosome 11. With approximately 135 million base pairs and 1,400 genes, chromosome 11 comprises approximately 4% of human genomic DNA and is considered a gene and disease association dense chromosome. The chromosome 11 encoded Atm gene is important for regulation of cell cycle arrest and apoptosis following double strand DNA breaks. Atm mutation leads to the disorder known as ataxia-telangiectasia. The blood disorders Sickle cell anemia and thalassemia are caused by HBB gene mutations, while Wilms’ tumors, WAGR syndrome and Denys-Drash syndrome are associated with mutations of the WT1 gene. Jervell and Lange-Nielsen syndrome, Jacobsen syndrome, Niemann-Pick disease, hereditary angioedema and Smith-LemliOpitz syndrome are also associated with defects in chromosome 11-encoded genes.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconstitution

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.25-0.5 μg/ml, Mouse, Rat
Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/ml, Mouse, Rat
Direct ELISA, 0.1-0.5 μg/ml, Mouse

Validation Images & Assay Conditions

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Figure 1. Western blot analysis of Batf2 using anti-Batf2 antibody (A09891-2).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: rat C6 whole cell lysates,
Lane 2: rat brain tissue lysates,
Lane 3: mouse brain tissue lysates,
Lane 4: mouse kidney tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Batf2 antigen affinity purified polyclonal antibody (Catalog # A09891-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Batf2 at approximately 25 kDa. The expected band size for Batf2 is at 25 kDa.

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Figure 2. IHC analysis of Batf2 using anti-Batf2 antibody (A09891-2).
Batf2 was detected in a paraffin-embedded section of mouse kidney tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Batf2 Antibody (A09891-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.

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Figure 3. IHC analysis of Batf2 using anti-Batf2 antibody (A09891-2).
Batf2 was detected in a paraffin-embedded section of rat kidney tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Batf2 Antibody (A09891-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.

Gene/Protein Information For Batf2 (Source: Uniprot.org, NCBI)

Alternative Names

basic leucine zipper transcription factor, ATF-like 2; basic leucine zipper transcriptional factor ATF-like 2; B-ATF-2; MGC20410; SARI; suppressor of AP-1 regulated by IFN Batf2|B-ATF-2|basic leucine zipper transcription factor, ATF-like 2|basic leucine zipper transcriptional factor ATF-like 2

For more info on Batf2, check out the Batf2 Infographic

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In this infographic, you will see the following information for Batf2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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