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Product Info Summary
| SKU: | A02734 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | ELISA, IF, WB |
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Product info
Product Name
Anti-BANF1 Antibody
SKU/Catalog Number
A02734
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-BANF1 Antibody (Catalog # A02734). Tested in ELISA, WB, IF applications. This antibody reacts with Human.
Storage & Handling
BANF1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Cite This Product
Anti-BANF1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A02734)
Host
Rabbit
Contents
BANF1 Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Anti-BANF1 antibody was raised against a peptide corresponding to 15 amino acids near the carboxy terminus of human BANF1. The immunogen is located within the last 50 amino acids of BANF1.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
Multiple isoforms of CLEC7A are known to exist.
Reactive Species
A02734 is reactive to BANF1 in Human
Reconstitution
Observed Molecular Weight
68 kDa
Calculated molecular weight
10059 MW
Background of BANF1
Barrier-to-autointegration factor 1 (BANF1) is a conserved chromatin protein that non-specifically binds double-stranded DNA. BANF1 also interacts with a family of nuclear proteins that include LAP2, emerin, and MAN1. It is also a host cell component of retroviral pre-integration complexes (PICs), including that of HIV. BANF1 will bind to p55 Gag (the structural precursor of HIV-1 virions) as well as its cleaved product matrix. In addition to being a host cell component of the PIC, it is thought that BANF1 is also present at low levels in incoming virions, and thus might contribute to the assembly or activity of HIV-1 PICs through direct binding to matrix as well as DNA.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A02734 is guaranteed for ELISA, IF, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 0.5-2 μg/mL; IF: 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Independent Antibody Validation (IAV) via Protein Expression Profile in Human, Mouse and Rat Cell Lines
Loading: 15 μg of lysates per lane.Antibodies: BANF1 A02734 (2 μg/mL), BANF1, (2 μg/mL) and beta-actin (1.5 μg/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
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Western Blot Validation in Human Kidney Tissue Lysate
Loading: 15 μg of lysates per lane.Antibodies: BANF1 A02734 (A: 0.5 μg/mL and B: 1 μg/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
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Western Blot Validation in Human Kidney Tissue Lysate
Loading: 15 μg of lysates per lane. Antibodies: BANF1 A02734 (A: 0.5 μg/mL and B: 1 μg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
Protein Target Info & Infographic
Gene/Protein Information For BANF1 (Source: Uniprot.org, NCBI)
Gene Name
BANF1
Full Name
Barrier-to-autointegration factor
Weight
10059 MW
Superfamily
BAF family
Alternative Names
BAF, NGPS, BCRP1, D14S1460, BAF, BCRG1, Barrier-to-autointegration factor, Breakpoint cluster region protein 1 BANF1 BAF, BCRP1, D14S1460, NGPS BAF nuclear assembly factor 1 barrier-to-autointegration factor|barrier to autointegration factor 1|breakpoint cluster region protein 1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on BANF1, check out the BANF1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for BANF1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-BANF1 Antibody (A02734)
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