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Product Info Summary
| SKU: | A01163 |
|---|---|
| Size: | 50µg |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | IP, IF, IHC, WB |
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Product info
Product Name
Anti-Bak BAK1 Antibody
SKU/Catalog Number
A01163
Size
50µg
Form
Liquid. In PBS containing 50% glycerol and 0.09% sodium azide.
Description
Boster Bio Anti-Bak BAK1 Antibody catalog # A01163. Tested in IP, IF, IHC, WB applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Bak BAK1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01163)
Host
Rabbit
Contents
Liquid. In PBS containing 50% glycerol and 0.09% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthetic peptide corresponding to a portion of human Bak.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A01163 is reactive to BAK1 in Human
Reconstitution
Calculated molecular weight
83786 MW
Background of BAK
Anti-Glycogen Synthase 1 pS641 antibody is validated by IHC, Western Blot and ELISA. Human muscle glycogen synthase (GS) is responsible for the biosynthesis of glycogen from phosphorylated glucose units. Mammalian liver and muscle contain GS consisting of four subunits with a total molecular weight of 360,000. GS is subject to regulation through both allosteric and covalent modification and occurs in two forms: the phosphorylated inactive form, and the dephosphorylated active form. GS is inactivated by the serine/threonine kinase called glycogen synthase kinase-3 that mainly functions to phosphorylate muscle glycogen synthase. This antibody is specific for the phosphorylated form of GS at S641. Phosphorylation of GS at S641 has been associated with Antiphospholipid Antibody Syndrome.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A01163 is guaranteed for IP, IF, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry (1:50)
Immunoprecipitation (1:100)
Western Blot (1:1,000, ECL)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application.
Validation Images & Assay Conditions
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Immunofluorescent analysis (confocal) staining of He La cells using Bak pAb (green)
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Figure 2. IHC analysis of BAK1 using anti-BAK1 antibody (A01163).
BAK1 was detected in paraffin-embedded section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-BAK1 Antibody (A01163) overnight at 4°C. Biotinylated goat anti Rabbit IgG antibody was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
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Figure 3. Western blot analysis of BAK1 using anti-BAK1 antibody (A01163).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-BAK1 antigen affinity purified polyclonal antibody (Catalog # A01163) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-Rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # SA1022) with Tanon 5200 system. A specific band was detected for BAK1.
Protein Target Info & Infographic
Gene/Protein Information For BAK1 (Source: Uniprot.org, NCBI)
Gene Name
BAK1
Full Name
Bcl-2 homologous antagonist/killer
Weight
83786 MW
Superfamily
Bcl-2 family
Alternative Names
muscle glycogen synthase pS641, glycogen synthase pS641, Glycogen, Glycogen synthase 1 (muscle), Glycogen synthase 1, Glycogen synthase1, GYS 1, GYS-1, GYS1, Starch synthase muscle BAK1 BAK, BAK-LIKE, BCL2L7, CDN1 BCL2 antagonist/killer 1 bcl-2 homologous antagonist/killer|BCL2-like 7 protein|apoptosis regulator BAK|bcl-2-like protein 7|bcl2-L-7|pro-apoptotic protein BAK
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on BAK1, check out the BAK1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for BAK1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Bak BAK1 Antibody (A01163)
Hello CJ!
A01163 has been cited in 2 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Minicircle-oriP-IFN?: A Novel Targeted Gene Therapeutic System for EBV Positive Human Nasopharyngeal Carcinoma
BAK overexpression mediates p53-independent apoptosis inducing effects on human gastric cancer cells
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