Anti-Progesterone Receptor (Marker of Progestin Dependence) PGR Monoclonal Antibody

Product Name

Anti-Progesterone Receptor (Marker of Progestin Dependence) PGR Monoclonal Antibody

View all PGR Antibodies

SKU/Catalog Number

M00541

Size

100ug/vial

Form

Liquid

Description

Boster Bio Anti-Progesterone Receptor (Marker of Progestin Dependence) PGR Monoclonal Antibody (Catalog # M00541). Tested in WB, IHC applications. This antibody reacts with Human.

Storage & Handling

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Cite This Product

Anti-Progesterone Receptor (Marker of Progestin Dependence) PGR Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00541)

Host

Mouse

Contents

Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Clonality

Monoclonal

Clone Number

Clone: PR501

Isotype

IgG1, kappa

Immunogen

Recombinant human Progesterone Receptor protein

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

Does not cross-react with primate, avian or amphibian GR.

Reactive Species

M00541 is reactive to PGR in Human

Applications

M00541 is guaranteed for IHC, WB Boster Guarantee

Observed Molecular Weight

Calculated molecular weight

98981 MW

Background of PGR

This monoclonal antibody is specific to progesterone receptor and shows minimal cross-reaction with other members of the family. Progesterone receptor is expressed as two major isoforms, PR-A (81kDa) and PR-B (116kDa). Expression of PgR has been suggested to reflect a intact estrogen regulatory machinery and therefore, predict better clinical response to endocrine therapy than ER alone.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconstitution

Reconstitute with distilled water.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western Blot (1-2ug/ml for 2 hours at Room Temp)
Immunohistochemistry (Formalin-fixed) (1-2ug/ml) (Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Validation Images & Assay Conditions

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Formalin-fixed, paraffin-embedded normal human breast (left) and invasive ductal carcinoma (right) stained with Anti-Progesterone receptor Antibody (PR501).

Gene/Protein Information For PGR (Source: Uniprot.org, NCBI)

Alternative Names

NR3C3; NR3C3PRNuclear receptor subfamily 3 group C member 3; PGR; Progesterone R; progesterone receptor; ProgesteroneR PGR NR3C3, PR progesterone receptor progesterone receptor|nuclear receptor subfamily 3 group C member 3

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In this infographic, you will see the following information for PGR: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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