Product Info Summary
SKU: | A18982 |
---|---|
Size: | 100 µl |
Reactive Species: | Human |
Host: | Rabbit |
Application: | IHC |
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Product info
Product Name
Anti-B melanoma antigen 4 BAGE4 Antibody
SKU/Catalog Number
A18982
Size
100 µl
Form
Liquid
Description
Boster Bio Anti-B melanoma antigen 4 BAGE4 Antibody (Catalog # A18982). Tested in IHC applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-B melanoma antigen 4 BAGE4 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A18982)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthesized peptide derived from internal of human BAGE4.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A18982 is reactive to BAGE4 in Human
Reconstitution
Calculated molecular weight
4230 MW
Background of BAGE4
Unknown. Candidate gene encoding tumor antigens.
Ruault M., Submitted (JAN-2001) to the EMBL/GenBank/DDBJ databases.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A18982 is guaranteed for IHC Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry: 1:50~1:100
Validation Images & Assay Conditions
Click image to see more details
Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue using BAGE4 antibody A18982.
Protein Target Info & Infographic
Gene/Protein Information For BAGE4 (Source: Uniprot.org, NCBI)
Gene Name
BAGE4
Full Name
B melanoma antigen 4
Weight
4230 MW
Superfamily
BAGE family
Alternative Names
B melanoma antigen 4;Cancer/testis antigen 2.4;CT2.4;BAGE4;MLL3P; BAGE4 CT2.4, MLL3P BAGE family member 4 B melanoma 4|B melanoma family member 4|MLL3 pseudogene|cancer/testis 2.4|cancer/testis family 2, member 4
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on BAGE4, check out the BAGE4 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for BAGE4: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-B melanoma antigen 4 BAGE4 Antibody (A18982)
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Customer Q&As
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5 Customer Q&As for Anti-B melanoma antigen 4 BAGE4 Antibody
Question
We are currently using anti-BAGE4 antibody A18982 for human tissue, and we are happy with the IHC results. The species of reactivity given in the datasheet says human. Is it likely that the antibody can work on monkey tissues as well?
Verified Customer
Verified customer
Asked: 2020-04-10
Answer
The anti-BAGE4 antibody (A18982) has not been validated for cross reactivity specifically with monkey tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in monkey you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-04-10
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for testis using anti-BAGE4 antibody A18982. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-02-22
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-02-22
Question
See attached the WB image, lot number and protocol we used for testis using anti-BAGE4 antibody A18982. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-01-23
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-01-23
Question
I was wanting to use your anti-BAGE4 antibody for IHC for human testis on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human testis identification?
R. Miller
Verified customer
Asked: 2017-12-27
Answer
As indicated on the product datasheet, A18982 anti-BAGE4 antibody has been tested for IHC on human tissues. We have an innovator award program that if you test this antibody and show it works in human testis in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2017-12-27
Question
Would anti-BAGE4 antibody A18982 work on dog IHC with testis?
A. Thomas
Verified customer
Asked: 2014-01-28
Answer
Our lab technicians have not validated anti-BAGE4 antibody A18982 on dog. You can run a BLAST between dog and the immunogen sequence of anti-BAGE4 antibody A18982 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated dog samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in dog testis in IHC, you can get your next antibody for free.
Boster Scientific Support
Answered: 2014-01-28