Anti-Cullin 2/CUL2 Antibody Picoband®

Product Name

Anti-Cullin 2/CUL2 Antibody Picoband®

View all Cullin 2 Antibodies

SKU/Catalog Number

PA1877

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-Cullin 2/CUL2 Antibody catalog # PA1877. Tested in WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Cullin 2/CUL2 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1877)

Host

Rabbit

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

A synthetic peptide corresponding to a sequence in the middle region of human Cullin 2, different from the related mouse and rat sequences by one amino acid.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins

Reactive Species

PA1877 is reactive to CUL2 in Human, Mouse, Rat

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Observed Molecular Weight

37 kDa

Calculated molecular weight

86983 MW

Background of Cullin 2

Cullin 2 (CUL2) is a protein that in humans is encoded by the CUL2 gene. Using immunofluorescence, they showed that CUL2 is a cytosolic protein that can be translocated to the nucleus by VHL. By fluorescence in situ hybridization, Clifford et al. (1999)mapped the CUL2 gene to 10p11.2-p11.1, a region reported to show loss of heterozygosity (LOH) in several forms of human cancer, including non-clear cell renal cell carcinoma. Pause et al. (1997)suggested that CUL2 is a candidate tumor suppressor gene, as has been proposed for CUL1 (603134). Lonergan et al. (1998) demonstrated that formation of the VBC-CUL2 complexes is linked to the regulation of hypoxia-inducible mRNAs by VHL. Cul2 was one of several proteins required for degradation of a class of RNA-binding germline proteins in somatic cells of the early blastomere.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

PA1877 is guaranteed for WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml, Human, Rat, Mouse

Positive Control

WB: Rat Brain Tissue, A431 Cell, SMMC Cell, HELA Cell, COLO320 Cell

Validation Images & Assay Conditions

Gene/Protein Information For CUL2 (Source: Uniprot.org, NCBI)

Alternative Names

Cullin-2;CUL-2;CUL2; CUL2 cullin 2 cullin-2|CUL-2|testis secretory sperm-binding protein Li 238E

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

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