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Product Info Summary
| SKU: | M02675-3 |
|---|---|
| Size: | 100uL |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | IHC, WB |
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Product info
Product Name
Anti-CD3E Rabbit Monoclonal Antibody, Clone#RM344
SKU/Catalog Number
M02675-3
Size
100uL
Form
Liquid
Description
Boster Bio Anti-CD3E Rabbit Monoclonal Antibody, Clone#RM344 (Catalog # M02675-3). Tested in IHC, WB applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-CD3E Rabbit Monoclonal Antibody, Clone#RM344 (Boster Biological Technology, Pleasanton CA, USA, Catalog # M02675-3)
Host
Rabbit
Contents
50% Glycerol/PBS with 1% BSA and 0.09% sodium azide
Clonality
Monoclonal
Clone Number
RM344
Isotype
Rabbit IgG
Immunogen
A peptide corresponding to the C-terminus of human CD3 epsilon chain.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
This antibody reacts to the cytoplasmic domain of human CD3 epsilon chain.
Reactive Species
M02675-3 is reactive to CD3E in Human
Reconstitution
Calculated molecular weight
23147 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M02675-3 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry (IHC): 1:100-1:200 dilution
WB: 1:1000-1:2000 dilution.
Validation Images & Assay Conditions
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Figure 1. Western Blotting result
Western Blot of Jurkat cell lysate using anti-CD3e rabbit monoclonal antibody (Clone RM344) at a 1:1000 dilution.
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Figure 2. IHC result
Immunohistochemical staining of formalin fixed and paraffin embedded human Tonsil tissue section using anti-CD3e rabbit monoclonal antibody (Clone RM344) at a 1:200 dilution.
Protein Target Info & Infographic
Gene/Protein Information For CD3E (Source: Uniprot.org, NCBI)
Gene Name
CD3E
Full Name
T-cell surface glycoprotein CD3 epsilon chain
Weight
23147 MW
Alternative Names
T-cell surface glycoprotein CD3 epsilon chain;T-cell surface antigen T3/Leu-4 epsilon chain;CD3e;CD3E;T3E; CD3E IMD18, T3E, TCRE CD3e molecule T-cell surface glycoprotein CD3 epsilon chain|CD3-epsilon|CD3e , epsilon polypeptide (TiT3 complex)|CD3e molecule, epsilon (CD3-TCR complex)|T-cell receptor complex, epsilon subunit of T3|T-cell surface T3/Leu-4 epsilon chain
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CD3E, check out the CD3E Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CD3E: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-CD3E Rabbit Monoclonal Antibody, Clone#RM344 (M02675-3)
Hello CJ!
M02675-3 has been cited in 1 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Li Y, Geng S, Yin Q, Chen S, Yang L, Wu X, Li B, Du X, Schmidt Ca, Przybylski Gk. J Transl Med. 2010 May 14;8:47. Doi: 10.1186/1479-5876-8-47. Decreased Level Of Recent Thymic Emigrants In Cd4+ And Cd8+T Cells From Cml Patients.
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Customer Reviews
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Customer Q&As
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16 Customer Q&As for Anti-CD3E Rabbit Monoclonal Antibody, Clone#RM344
Question
My question regarding product M02675-3, anti-CD3E Rabbit Monoclonal antibody, Clone#RM344. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2020-03-27
Answer
We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M02675-3 anti-CD3E Rabbit Monoclonal antibody, Clone#RM344, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2020-03-27
Question
We bought anti-CD3E Rabbit Monoclonal antibody, Clone
Verified Customer
Verified customer
Asked: 2020-02-21
Answer
I have checked the website and datasheets of our anti-CD3E Rabbit Monoclonal antibody, Clone
Boster Scientific Support
Answered: 2020-02-21
Question
I have attached the WB image, lot number and protocol we used for blood using anti-CD3E Rabbit Monoclonal antibody, Clone#RM344 M02675-3. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2020-02-11
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-02-11
Question
Does anti-CD3E Rabbit Monoclonal antibody, Clone#RM344 M02675-3 work for IHC with blood?
Verified Customer
Verified customer
Asked: 2020-01-02
Answer
According to the expression profile of blood, CD3E is highly expressed in blood. So, it is likely that anti-CD3E Rabbit Monoclonal antibody, Clone#RM344 M02675-3 will work for IHC with blood.
Boster Scientific Support
Answered: 2020-01-02
Question
I see that the anti-CD3E Rabbit Monoclonal antibody, Clone#RM344 M02675-3 works with IHC, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-12-03
Answer
You can find protocols for IHC on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2019-12-03
Question
Does M02675-3 anti-CD3E Rabbit Monoclonal antibody, Clone#RM344 work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-11-07
Answer
It shows on the product datasheet, M02675-3 anti-CD3E Rabbit Monoclonal antibody, Clone#RM344 as been validated on IHC. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-11-07
Question
I appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for blood using anti-CD3E Rabbit Monoclonal antibody, Clone#RM344 M02675-3. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-07-11
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-07-11
Question
I was wanting to use your anti-CD3E Rabbit Monoclonal antibody, Clone#RM344 for IHC for human blood on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human blood identification?
Verified Customer
Verified customer
Asked: 2019-05-20
Answer
As indicated on the product datasheet, M02675-3 anti-CD3E Rabbit Monoclonal antibody, Clone#RM344 has been tested for IHC, WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human blood in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-05-20
Question
We have been able to see staining in human leukemic t-cell. Do you have any suggestions? Is anti-CD3E Rabbit Monoclonal antibody, Clone#RM344 supposed to stain leukemic t-cell positively?
Verified Customer
Verified customer
Asked: 2018-12-14
Answer
From literature leukemic t-cell does express CD3E. From Uniprot.org, CD3E is expressed in leukocyte, blood, thymus, leukemic t-cell, among other tissues. Regarding which tissues have CD3E expression, here are a few articles citing expression in various tissues:
Blood, Pubmed ID: 3267235, 15489334
Leukemic T-cell, Pubmed ID: 15144186, 19690332
Thymus, Pubmed ID: 14702039
Boster Scientific Support
Answered: 2018-12-14
Question
My lab would like to test anti-CD3E Rabbit Monoclonal antibody, Clone#RM344 M02675-3 on human blood for research purposes, then I may be interested in using anti-CD3E Rabbit Monoclonal antibody, Clone#RM344 M02675-3 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2018-01-26
Answer
The products we sell, including anti-CD3E Rabbit Monoclonal antibody, Clone#RM344 M02675-3, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2018-01-26
Question
Is this M02675-3 anti-CD3E Rabbit Monoclonal antibody, Clone#RM344 reactive to the isotypes of CD3E?
S. Evans
Verified customer
Asked: 2018-01-08
Answer
The immunogen of M02675-3 anti-CD3E Rabbit Monoclonal antibody, Clone#RM344 is A peptide corresponding to the C-terminus of human CD3 epsilon chain. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2018-01-08
Question
Do you have a BSA free version of anti-CD3E Rabbit Monoclonal antibody, Clone#RM344 M02675-3 available?
E. Krishna
Verified customer
Asked: 2016-03-08
Answer
Thank you for your recent telephone inquiry. I can confirm that some lots of this anti-CD3E Rabbit Monoclonal antibody, Clone#RM344 M02675-3 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2016-03-08
Question
Is a blocking peptide available for product anti-CD3E Rabbit Monoclonal antibody, Clone#RM344 (M02675-3)?
C. Li
Verified customer
Asked: 2014-12-18
Answer
We do provide the blocking peptide for product anti-CD3E Rabbit Monoclonal antibody, Clone#RM344 (M02675-3). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2014-12-18
Question
My team were well pleased with the WB result of your anti-CD3E Rabbit Monoclonal antibody, Clone
T. Huang
Verified customer
Asked: 2014-12-09
Answer
From what I have seen in literature, leukemic t-cell does express CD3E. Generally CD3E expresses in cell membrane. Regarding which tissues have CD3E expression, here are a few articles citing expression in various tissues:
Blood, Pubmed ID: 3267235, 15489334
Leukemic T-cell, Pubmed ID: 15144186, 19690332
Thymus, Pubmed ID: 14702039
Boster Scientific Support
Answered: 2014-12-09
Question
We are currently using anti-CD3E Rabbit Monoclonal antibody, Clone#RM344 M02675-3 for human tissue, and we are happy with the IHC results. The species of reactivity given in the datasheet says human. Is it likely that the antibody can work on goat tissues as well?
D. Walker
Verified customer
Asked: 2013-11-25
Answer
The anti-CD3E Rabbit Monoclonal antibody, Clone#RM344 (M02675-3) has not been tested for cross reactivity specifically with goat tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in goat you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2013-11-25
Question
I am interested in using your anti-CD3E Rabbit Monoclonal antibody, Clone#RM344 for positive regulation of gene expression studies. Has this antibody been tested with western blotting on human tonsil tissue? We would like to see some validation images before ordering.
H. Wu
Verified customer
Asked: 2013-02-25
Answer
We appreciate your inquiry. This M02675-3 anti-CD3E Rabbit Monoclonal antibody, Clone#RM344 is tested on jurkat cell lysate, human tonsil tissue. It is guaranteed to work for IHC, WB in human. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2013-02-25
