Anti-BAG2 Antibody Picoband™ (monoclonal, 8F11G2)

Product Name

Anti-BAG2 Antibody Picoband™ (monoclonal, 8F11G2)

View all BAG2 Antibodies

SKU/Catalog Number

M04933-2

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-BAG2 Antibody Picoband™ (monoclonal, 8F11G2) catalog # M04933-2. Tested in Flow Cytometry, WB applications. This antibody reacts with Human, Mouse.

Storage & Handling

At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Cite This Product

Anti-BAG2 Antibody Picoband™ (monoclonal, 8F11G2) (Boster Biological Technology, Pleasanton CA, USA, Catalog # M04933-2)

Host

Mouse

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Clonality

Monoclonal

Clone Number

8F11G2

Isotype

Mouse IgG1

Immunogen

E.coli-derived human BAG2 recombinant protein (Position: M1-N211). Human BAG2 shares 93.4% amino acid (aa) sequence identity with mouse BAG2.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

M04933-2 is reactive to BAG2 in Human, Mouse

Applications

M04933-2 is guaranteed for Flow Cytometry, WB Boster Guarantee

Observed Molecular Weight

24 kDa

Calculated molecular weight

Background of BAG2

BAG family molecular chaperone regulator 2 is a protein that in humans is encoded by the BAG2 gene. The predicted BAG2 protein contains 211 amino acids. The BAG domains of BAG1, BAG2, and BAG3 interact specifically with the Hsc70 ATPase domain in vitro and in mammalian cells. All 3 proteins bind with high affinity to the ATPase domain of Hsc70 and inhibit its chaperone activity in a Hip-repressible manner. The functional antagonisms displayed between BAG family proteins and Hip suggest that a proper balance of these 2 types of protein is required for achieving optimal cycles of substrate binding and release required for inducting conformational changes in proteins, with Hip promoting peptide substrate binding by Hsc70/Hsp70 and BAG family proteins promoting dissociation.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconstitution

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.25-0.5 μg/ml, Human, Mouse
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human

Validation Images & Assay Conditions

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Figure 1. Western blot analysis of BAG2 using anti-BAG2 antibody (M04933-2).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Jurkat whole cell lysates,
Lane 2: human Hela whole cell lysates,
Lane 3: human HepG2 whole cell lysates,
Lane 4: human K562 whole cell lysates,
Lane 5: mouse liver tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-BAG2 antigen affinity purified monoclonal antibody (Catalog # M04933-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for BAG2 at approximately 24 kDa. The expected band size for BAG2 is at 24 kDa.

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Figure 2. Flow Cytometry analysis of K562 cells using anti-BAG2 antibody (M04933-2).
Overlay histogram showing K562 cells stained with M04933-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-BAG2 Antibody (M04933-2, 1 μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-mouse IgG (BA1126, 5-10 μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was mouse IgG (1 μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.

Gene/Protein Information For BAG2 (Source: Uniprot.org, NCBI)

Alternative Names

BAG family molecular chaperone regulator 2; BAG-2; BAG-family molecular chaperone regulator-2; Bcl-2-associated athanogene 2; BCL2-associated athanogene 2; dJ417I1.2 (BAG-family molecular chaperone regulator 2); dJ417I1.2; KIAA0576; MGC149462 BAG2 BAG-2, dJ417I1.2 BAG cochaperone 2 BAG family molecular chaperone regulator 2|BCL2 associated athanogene 2|bcl-2-associated athanogene 2|dJ417I1.2 (BAG-family molecular chaperone regulator 2)

For more info on BAG2, check out the BAG2 Infographic

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In this infographic, you will see the following information for BAG2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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