BAG2 Antibodies

BAG family molecular chaperone regulator 2 (BAG2)

Co-chaperone for HSP70 and HSC70 chaperone proteins.

Acts as a nucleotide-exchange factor (NEF) promoting the release of ADP from the HSP70 and HSC70 proteins thereby triggering client/substrate protein release (PubMed:24318877, PubMed:9873016).

.

Gene Information

Human
Gene Name:	BAG2
Uniprot:	O95816
Entrez:	9532

Family

Belongs to:
No superfamily

Alternative Names

BAG family molecular chaperone regulator 2; BAG-2; BAG-family molecular chaperone regulator-2; Bcl-2-associated athanogene 2; BCL2-associated athanogene 2; dJ417I1.2 (BAG-family molecular chaperone regulator 2); dJ417I1.2; KIAA0576; MGC149462

Molecular Weight

Mass (kDA):
23.772 kDA

Genomic Context

Human
Location:	6p12.1
Sequence:	6; NC_000006.12 (57172326..57189833)

Diseases

• Muscle Twitch
• Neoplasms
• Neoplasm Metastasis
• Malignant Neoplasms
• Cystic Fibrosis
• Carcinoma
• Fibrosis
• Decerebrate State
• Cell Transformation, Neoplastic
• Malignant Paraganglionic Neoplasm
• Diseases, Protein Folding

• Neurodegenerative Disorders
• Malignant Neoplasm Of Brain
• Colonic Neoplasms
• Neuromuscular Diseases
• Malignant Neoplasm Of Urinary Bladder
• Decreased Vascular Flow

Interacting Proteins

• DAPK1
• DNAJC7
• HSPA8
• LRRK2
• SGTA

• STUB1

Pathways

• Innervation
• Localization
• Locomotion
• Protein Folding
• Transport
• Cellular Senescence
• Senescence
• Swimming
• Cell Death
• Rna Interference
• Spindle Assembly
• Aging
• Dna Repair

• Protein Transport
• Limb Development
• Myelination
• Cell Cycle Arrest
• Regeneration
• Flight
• Cellular Localization

PTMs

• Phosphorylation

• Ubiquitination

• Cleavage

Research Areas

• Apoptosis

For details, visit:
bosterbio.com/bosterbio-gene-info-cards/BAG2

Boster Bio - Best Uses Of The BAG2 Marker

Antibodies against BAG2 are important for a number of applications in immunology. Boster bio develops high affinity primary antibodies against the BAG2 Marker and validates them on multiple platforms with known positive and negative samples. Boster rewards scientists for first-time reviews of their products with product credits. This program is open to scientists world-wide. Read on to learn more about Boster bio and the Anti-BAG2 Marker.

Anti-BAG2 Marker

When looking for an antibody, you should look for a product that has high affinity and specificity. Boster Bio antibodies are highly cited and have been validated using several protocols, including Western Blotting, Immunohistochemistry, and ELISA. Their high affinity and specificity means that they can be trusted by researchers around the world. Boster Bio offers a wide range of conjugates for this antibody.

This monoclonal antibody is suitable for use in IHC applications and is non-hazardous. It can be stored at -20°C and has a shelf-life of six months. Boster Bio Anti-BAG2 Marker is composed of 5 mg BSA and 0.05mg thimerosal and is formulated to react with human, mouse, and rat BAG2 (1-14aa MAQAKINAKINANEGR).

High-affinity primary antibodies

Boster Bio high-affinity primary antibodies are rabbit mAbs that are made by their proprietary PCD platform. This proprietary chemistry helps to develop antibodies with the highest affinity and suitability for use in Diagnostics & Therapeutics. The process involves incubating splenocytes with flurochrome conjugated antigens and plasma cells, and selecting clones with the highest brightness and purity for downstream screening.

With over 20 years of experience in antibody development and manufacturing, Boster has developed a range of ELISA kits with excellent sensitivity, specificity, and stability. The Boster Picokine(tm) ELISA kits are trusted by over 14,000 scientists and are designed for use in immunoprecipitation experiments and are highly sensitive. These ELISA kits allow for the detection of native forms of proteins, with picogram and subpicogram level sensitivity.

Polyclonal antibodies are acceptable for research use and can serve as a stepping stone to producing monoclonal antibodies. However, because the clones do not amplify, the polyclonal antibodies are mainly used for the detection of secreted proteins or biomarkers. In addition, polyclonal antibodies can be more expensive than monoclonal antibodies and require close follow-up.

Enzyme-linked immunosorbent assays use both primary and secondary antibodies to detectcause resence or absence of target antigens. The sensitivity of these assays depends on how specificcauseantibodies are and the detection system used. High-affinity primary antibodies can increase the sensitivity of enzyme-linked immunoassays by up to 30 times. The company has mastered the art of coating plates with high-affinity primary antibodies and is trusted by scientists worldwide.

Validation on WB

To validate a WB with the BAG2 marker, a sample was loaded onto four separate gels and analyzed for BAG expression. After SDS-PAGE, the BAG2 signal was quantified as the highest intensity. This signal was correlated with a lower intensity band of 25 kDa, which indicates that both bands were likely to be BAG2 in plasma. The BAG2 levels of control and PD subjects were both heterogeneous while PD samples were homogeneous and significantly low.

IHC

One study found that patients with higher levels of BAG2 had a worse prognosis than those with lower BAG2 levels. This finding indicates that BAG2 levels may serve as independent prognostic markers for HCC. BAG2 supports tumor proliferation and metastasis and acts as an oncogene across cancer cells. In addition to its prognostic value, BAG2 is also associated with poor patient survival.

BAG2 promotes proliferation and invasion of HCC. In addition, it regulates the ribosome biogenesis process. The BAG2 gene interacts with DCAF13, WDR12, NIFK, and RRS1, which inhibit ribosome biogenesis. These processes are gaining increasing attention in cancer research. The BAG2 gene is one of several novel tumor-specificcmarkers.

The BAG2 marker has many uses in immunohistochemistry (IHC). It is frequently used to detectcp21, an antigen that is found on the surface of colon cancer cells. In addition to the above-mentioned applications, BAG2 is used for the development of new drugs. The BAG2 gene is expressed in many tumors in the body, and IHC detects the level of activity or down-regulation in BAG2 in various types of tumors.

BAG2 expression was elevated in tumor tissues in a recent study. These findings are consistent with the results of other studies. BAG2 is an important tumor suppressor gene that regulates growth. In ause resent study, BAG2 was a highly specificcmarker in HCC tissues. The results suggest that BAG2 is an effectivecmarker for cancer detection. This study also has implications for developing cancer therapies. There is no clear consensus on the exact role of BAG2 in tumor cells, however.

ICC

The BAG2 gene, also known as BAGM2, is expressed abundantly in gastriccaumors. Its expression levels are closely related to the biological characteristics of gastricccancer, and BAG2 gene-expression studies suggest that it may have a therapeutic role in this disease. Molecular studies also suggest that BAG2 is associated with miR186, which is involved in gastricccancer progression. Here are some of the best uses of the BAG2 gene.

The BAG2 gene is expressed mainly in cells that express MG132. BAG2 expression levels of two or three are considered high, suggesting that the gene is highly expressed in these cells. Furthermore, BAG2 is involved in cross-talk with an aggresome-like structure. The BAG2 gene is expressed at levels that are comparable to the protein's survival time. Therefore, BAG2 has many potential applications in research and medicine.

BAG2 is produced by various mechanisms, including cellular stress. It is produced by the stress granules system, which is a type of canonical LLPS. Its DGtr is a quantitative measure of the changes in cellular interactions that result in BAG2 condenses. DGtr decreased by 0.17 kcal mole-1 after 15 min of incubation with sucrose. Moreover, the negative transfer free energy suggests the increased heterotypiccinteraction in BAG2 condenses.

Immunofluorescence

The BAG2 gene encodes a protein in the BAG family, molecular chaperone regulator 2. It contains 211 amino acids, which is predicted to have two BAG domains that interact with the Hsc70 ATPase domain. BAG family proteins inhibit Hsc70 chaperone activity in a Hip-repressible manner. Hip promotes dissociation, and BAG family proteins inhibit Hsc70 chaperone activity.