Anti-BAG1 Antibody Picoband™

Product Name

Anti-BAG1 Antibody Picoband™

View all Bag-1 Antibodies

SKU/Catalog Number

A02423-3

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-BAG1 Antibody Picoband™ catalog # A02423-3. Tested in WB applications. This antibody reacts with Human.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-BAG1 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A02423-3)

Host

Rabbit

Contents

Each vial contains 4mg Trehalose, 0.9mg NaCl and 0.2mg Na2HPO4.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

A synthetic peptide corresponding to a sequence at the C-terminus of human BAG1, which shares 91.4% and 88.6% amino acid (aa) sequence identity with mouse and rat BAG1, respectively.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins

Reactive Species

A02423-3 is reactive to BAG1 in Human

Applications

A02423-3 is guaranteed for WB Boster Guarantee

Observed Molecular Weight

33 kDa(S), 46 kDa(M), 56 kDa(L)

Calculated molecular weight

38779 MW

Background of Bag-1

BAG family molecular chaperone regulator 1 (BAG1) is a protein that in humans is encoded by the BAG1 gene. Human BAG1 is mapped to chromosome 9p12, a region associated with hereditary disorders that may involve developmental dysregulation of programmed cell death. The Bag1 protein is rich in glutamic acid residues. Its deduced 274-amino acid protein has a calculated molecular mass of 31 KD. Being the BCL-2-associated athanogene, Bag1 enhances the anti-apoptotic effects of BCL2 and represents a link between growth factor receptors and anti-apoptotic mechanisms.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.25-0.5μg/ml, Human

Validation Images & Assay Conditions

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Figure 1. Western blot analysis of BAG1 using anti-BAG1 antibody (A02423-3).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human Raji whole cell lysates,
Lane 3: human MCF-7 whole cell lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-BAG1 antigen affinity purified polyclonal antibody (Catalog # A02423-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for BAG1 at approximately 33 kDa(S), 46 kDa(M), 56 kDa(L). The expected band size for BAG1 is at 39 kDa.

Gene/Protein Information For BAG1 (Source: Uniprot.org, NCBI)

Alternative Names

BAG family molecular chaperone regulator 1; Bag1; Bag-1; Bcl-2 associating athanogene-1 protein; Bcl-2-associated athanogene 1; BCL2-associated athanogene; Bcl-2-binding protein; glucocortoid receptor-associated protein RAP46,46-KD; HAP; RAP46 BAG1 BAG-1, HAP, RAP46 BAG cochaperone 1 BAG family molecular chaperone regulator 1|BCL2 associated athanogene 1|BCL2-associated athanogene|Bcl-2 associating athanogene-1 protein|Bcl-2-binding protein|glucocortoid receptor-associated protein RAP46|receptor-associated protein, 46-KD

For more info on BAG1, check out the BAG1 Infographic

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In this infographic, you will see the following information for BAG1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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