Product Info Summary
SKU: | M02423 |
---|---|
Size: | 100uL |
Reactive Species: | Human |
Host: | Rabbit |
Application: | IHC, ICC, WB |
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Product info
Product Name
Anti-BAG-1L Rabbit Monoclonal Antibody, Clone#RM310
SKU/Catalog Number
M02423
Size
100uL
Form
Liquid
Description
Boster Bio Anti-BAG-1L Rabbit Monoclonal Antibody, Clone#RM310 (Catalog # M02423). Tested in IHC, WB, ICC applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-BAG-1L Rabbit Monoclonal Antibody, Clone#RM310 (Boster Biological Technology, Pleasanton CA, USA, Catalog # M02423)
Host
Rabbit
Contents
50% Glycerol/PBS with 1% BSA and 0.09% sodium azide
Clonality
Monoclonal
Clone Number
RM310
Isotype
Rabbit IgG
Immunogen
A peptide corresponding to residues near the N-terminus of human BAG-1L
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
This antibody reacts to human BAG-1L. It does not cross-react to BAG-1M or BAG-1S.
Reactive Species
M02423 is reactive to BAG1 in Human
Reconstitution
Calculated molecular weight
38779 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M02423 is guaranteed for IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry (IHC): 1:500-1:2000 dilution
WB: 1:1000-1:2000 dilution
Immunocytochemistry (ICC): 1:200-1:1000 dilution
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western Blotting result
Western Blot of HeLa cell lysate, using anti-BAG-1L rabbit monoclonal antibody (Clone RM310) at a 1:1000 dilution.
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Figure 2. IHC result
Immunohistochemical staining of formalin fixed and paraffin embedded 22RV1 cell section using anti-BAG-1L rabbit monoclonal antibody (Clone RM310) at a 1:2000 dilution.
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Figure 3. IHC result
Immunohistochemical staining of formalin fixed and paraffin embedded 22RV1 cell section using anti-BAG-1L rabbit monoclonal antibody (Clone RM310) at a 1:2000 dilution.
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Figure 4. IHC result
Immunohistochemical staining of formalin fixed and paraffin embedded BAG-1L overexpressing LNCaP cell section using anti-BAG-1L rabbit monoclonal antibody (Clone RM310) at a 1:2000 dilution.
Protein Target Info & Infographic
Gene/Protein Information For BAG1 (Source: Uniprot.org, NCBI)
Gene Name
BAG1
Full Name
BAG family molecular chaperone regulator 1
Weight
38779 MW
Alternative Names
BAG family molecular chaperone regulator 1;BAG-1;Bcl-2-associated athanogene 1;BAG1;HAP; BAG1 BAG-1, HAP, RAP46 BAG cochaperone 1 BAG family molecular chaperone regulator 1|BCL2 associated athanogene 1|BCL2-associated athanogene|Bcl-2 associating athanogene-1 protein|Bcl-2-binding protein|glucocortoid receptor-associated protein RAP46|receptor-associated protein, 46-KD
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on BAG1, check out the BAG1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for BAG1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-BAG-1L Rabbit Monoclonal Antibody, Clone#RM310 (M02423)
Hello CJ!
M02423 has been cited in 1 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Bai Yx, Yi Jl, Li Jf, Sui H. World J Gastroenterol. 2007 Jul 28;13(28):3883-5. Clinicopathologic Significance Of Bag1 And Timp3 Expression In Colon Carcinoma.
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Customer Q&As
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5 Customer Q&As for Anti-BAG-1L Rabbit Monoclonal Antibody, Clone#RM310
Question
Is this M02423 anti-BAG-1L Rabbit Monoclonal antibody, Clone#RM310 reactive to the isotypes of BAG1?
Verified Customer
Verified customer
Asked: 2020-03-24
Answer
The immunogen of M02423 anti-BAG-1L Rabbit Monoclonal antibody, Clone#RM310 is A peptide corresponding to residues near the N-terminus of human BAG-1L. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2020-03-24
Question
I was wanting to use your anti-BAG-1L Rabbit Monoclonal antibody, Clone#RM310 for WB for human mammary gland on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human mammary gland identification?
Verified Customer
Verified customer
Asked: 2019-12-25
Answer
It shows on the product datasheet, M02423 anti-BAG-1L Rabbit Monoclonal antibody, Clone#RM310 has been validated for IHC, WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human mammary gland in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-12-25
Question
Do you have a BSA free version of anti-BAG-1L Rabbit Monoclonal antibody, Clone#RM310 M02423 available?
Verified Customer
Verified customer
Asked: 2019-09-30
Answer
I appreciate your recent telephone inquiry. I can confirm that some lots of this anti-BAG-1L Rabbit Monoclonal antibody, Clone#RM310 M02423 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-09-30
Question
Thank you for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for mammary gland using anti-BAG-1L Rabbit Monoclonal antibody, Clone#RM310 M02423. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-08-14
Answer
Thank you for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-08-14
Question
Does anti-BAG-1L Rabbit Monoclonal antibody, Clone#RM310 M02423 work on zebrafish IHC with cervix carcinoma?
Verified Customer
Verified customer
Asked: 2018-11-07
Answer
Our lab technicians have not validated anti-BAG-1L Rabbit Monoclonal antibody, Clone#RM310 M02423 on zebrafish. You can run a BLAST between zebrafish and the immunogen sequence of anti-BAG-1L Rabbit Monoclonal antibody, Clone#RM310 M02423 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated zebrafish samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in zebrafish cervix carcinoma in IHC, you can get your next antibody for free.
Boster Scientific Support
Answered: 2018-11-07