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Product Info Summary
| SKU: | A00456-6 |
|---|---|
| Size: | 80 µl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | Flow Cytometry, IHC-P, WB |
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Product info
Product Name
Anti-B2M Antibody (C-term)
View all beta 2-Microglobulin Antibodies
SKU/Catalog Number
A00456-6
Size
80 µl
Form
Liquid
Description
Boster Bio Anti-B2M Antibody (C-term) (Catalog # A00456-6). Tested in WB, IHC-P, Flow Cytometry application(s). This antibody reacts with Human.
Storage & Handling
Maintain refrigerated at 2-8°C for up to 2 weeks. For long-term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
Cite This Product
Anti-B2M Antibody (C-term) (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00456-6)
Host
Rabbit
Contents
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
This B2M antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 86-118 amino acids from the C-terminal region of human B2M.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
A00456-6 is reactive to B2M in Human
Applications
A00456-6 is guaranteed for Flow Cytometry, IHC-P, WB Boster Guarantee
Observed Molecular Weight
Calculated molecular weight
13715 Da
Background of beta 2-Microglobulin
Beta-2-microglobulin is a serum protein found in association with the major histocompatibility complex (MHC) class I heavy chain on the surface of nearly all nucleated cells.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:1000
IHC-P: 1:10-1:50
FC: 1:10-1:50
Validation Images & Assay Conditions
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B2M Antibody (C-term) western blot analysis in Jurkat cell line lysates (35ug/lane). This demonstrates the B2M antibody detected the B2M protein (arrow).
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Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with B2M antibody (C-term) (Cat. #A00456-1), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
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B2M Antibody (C-term) (Cat. #A00456-1) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Protein Target Info & Infographic
Gene/Protein Information For B2M (Source: Uniprot.org, NCBI)
Gene Name
B2M
Full Name
Beta-2-microglobulin
Weight
13715 Da
Superfamily
beta-2-microglobulin family
Alternative Names
B2M; beta 2Microglobulin; beta 2-Microglobulin; beta chain of MHC class I molecules; beta-2-microglobin; beta-2-microglobulin B2M IMD43 beta-2-microglobulin beta-2-microglobulin|beta chain of MHC class I molecules|beta-2-microglobin
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on B2M, check out the B2M Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for B2M: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-B2M Antibody (C-term) (A00456-6)
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