Anti-Beta-2-microglobulin B2M Antibody

Product Name

Anti-Beta-2-microglobulin B2M Antibody

View all beta 2-Microglobulin Antibodies

SKU/Catalog Number

A00456-5

Size

100ul

Form

Liquid

Description

Boster Bio Anti-Beta-2-microglobulin B2M Antibody catalog # A00456-5. Tested in WB,ICC,IP applications. This antibody reacts with Human,Mouse,Rat.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Beta-2-microglobulin B2M Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00456-5)

Host

Rabbit

Contents

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Clonality

Polyclonal

Isotype

IgG

Immunogen

Synthesized peptide derived from human IL-8Rbeta around the phosphorylation site of S347.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

A00456-5 is reactive to B2M in Human, Mouse, Rat

Applications

A00456-5 is guaranteed for IP, ICC, WB Boster Guarantee

Observed Molecular Weight

Calculated molecular weight

13715 MW

Background of beta 2-Microglobulin

This antibody is suitable for Cancer, Immunology and Nuclear Signaling research. Fibroblast Activation Protein (FAP)  is expressed in the stroma of sites that are undergoing wound healing. In addition, it has recently been reported that FAP is expressed in the stroma of sites of metastatic disease. Inhibition of FAP may lead to a dramatic decrease in the number of metastatic osteosarcoma lung nodules.  FAP exists as an inactive monomer and when activated forms homodimers or heterodimers with DPP4. Multiple isoforms of FAP are reported as alternative splicing products from a common gene.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

View more details

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB: 1:1,000-1:2,000
ICC: 1:50-1:200

Validation Images & Assay Conditions

Click image to see more details

Figure 2. Immunocytochemistry staining of B2M using Anti-Beta-2-microglobulin B2M Antibody (A00456-5).

ICC staining beta 2 Microglobulin in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde

Click image to see more details

Figure 1. Western blotting validation for Anti-Beta-2-microglobulin B2M Antibody A00456-5

Western blot analysis of beta 2 Microglobulin on different lysates using anti-beta 2 Microglobulin antibody at 1/1

Gene/Protein Information For B2M (Source: Uniprot.org, NCBI)

Alternative Names

B2M; beta 2Microglobulin; beta 2-Microglobulin; beta chain of MHC class I molecules; beta-2-microglobin; beta-2-microglobulin B2M IMD43 beta-2-microglobulin beta-2-microglobulin|beta chain of MHC class I molecules|beta-2-microglobin

For more info on B2M, check out the B2M Infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for B2M: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

Take me to the B2M Infographic

Here are featured tools and databases that you might find useful.

• Boster's Pathways Library
• Protein Databases
• Bioscience Research Protocol Resources
• Data Processing & Analysis Software
• Photo Editing Software
• Scientific Literature Resources
• Research Paper Management Tools
• Molecular Biology Software
• Primer Design Tools
• Bioinformatics Tools
• Phylogenetic Tree Analysis