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Product Info Summary
| SKU: | A06845 |
|---|---|
| Size: | 100 µl |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-b-Enolase (ENO-3) Antibody
SKU/Catalog Number
A06845
Size
100 µl
Form
Liquid
Description
Boster Bio Anti-b-Enolase (ENO-3) Antibody (Catalog # A06845). Tested in WB applications. This antibody reacts with Human, Mouse.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-b-Enolase (ENO-3) Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A06845)
Host
Rabbit
Contents
Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Peptide sequence around aa.49~53(L-R-D-G-D) derived from Human b-Enolase(ENO-3).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A06845 is reactive to ENO3 in Human, Mouse
Reconstitution
Observed Molecular Weight
39 kDa
Calculated molecular weight
65034 MW
Background of ENO3
Appears to have a function in striated muscle development and regeneration.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A06845 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Predicted MW: 45kd
Western blotting: 1:500~1:1000
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of ENO3 using anti-ENO3 antibody (A06845).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ENO3 antigen affinity purified polyclonal antibody (Catalog # A06845) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-Rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # SA1022) with Tanon 5200 system. A specific band was detected for ENO3.
Protein Target Info & Infographic
Gene/Protein Information For ENO3 (Source: Uniprot.org, NCBI)
Gene Name
ENO3
Full Name
Beta-enolase
Weight
65034 MW
Superfamily
enolase family
Alternative Names
Immunoglobulin superfamily member 8;IgSF8;CD81 partner 3;Glu-Trp-Ile EWI motif-containing protein 2;EWI-2;Keratinocytes-associated transmembrane protein 4;KCT-4;LIR-D1;Prostaglandin regulatory-like protein;PGRL;CD316;IGSF8;CD81P3, EWI2, KCT4; ENO3 GSD13, MSE enolase 3 beta-enolase|2-phospho-D-glycerate hydrolyase|enolase 3 (beta, muscle)|muscle enriched enolase|muscle-specific enolase|skeletal muscle enolase
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on ENO3, check out the ENO3 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for ENO3: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-b-Enolase (ENO-3) Antibody (A06845)
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No publications found for A06845
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Customer Q&As
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6 Customer Q&As for Anti-b-Enolase (ENO-3) Antibody
Question
We are currently using anti-b-Enolase (ENO-3) antibody A06845 for mouse tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human, mouse. Is it true that the antibody can work on zebrafish tissues as well?
Verified Customer
Verified customer
Asked: 2020-01-20
Answer
The anti-b-Enolase (ENO-3) antibody (A06845) has not been validated for cross reactivity specifically with zebrafish tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in zebrafish you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-01-20
Question
We are interested in to test anti-b-Enolase (ENO-3) antibody A06845 on mouse skeletal muscle for research purposes, then I may be interested in using anti-b-Enolase (ENO-3) antibody A06845 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-03-08
Answer
The products we sell, including anti-b-Enolase (ENO-3) antibody A06845, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-03-08
Question
Please see the WB image, lot number and protocol we used for skeletal muscle using anti-b-Enolase (ENO-3) antibody A06845. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2017-11-21
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2017-11-21
Question
Does anti-b-Enolase (ENO-3) antibody A06845 work on pig WB with skeletal muscle tissue of rectus abdominis?
Verified Customer
Verified customer
Asked: 2017-08-01
Answer
Our lab technicians have not tested anti-b-Enolase (ENO-3) antibody A06845 on pig. You can run a BLAST between pig and the immunogen sequence of anti-b-Enolase (ENO-3) antibody A06845 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated pig samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in pig skeletal muscle tissue of rectus abdominis in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2017-08-01
Question
Does A06845 anti-b-Enolase (ENO-3) antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
W. Miller
Verified customer
Asked: 2015-06-10
Answer
You can see on the product datasheet, A06845 anti-b-Enolase (ENO-3) antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2015-06-10
Question
Is this A06845 anti-b-Enolase (ENO-3) antibody reactive to the isotypes of ENO3?
A. Mangal
Verified customer
Asked: 2014-02-28
Answer
The immunogen of A06845 anti-b-Enolase (ENO-3) antibody is Peptide sequence around aa.49~53 (L-R-D-G-D) derived from Human b-Enolase (ENO-3) . Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2014-02-28
