Product Info Summary
SKU: | A08235 |
---|---|
Size: | 100 µl |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | WB |
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Product info
Product Name
Anti-AZI2 Antibody
SKU/Catalog Number
A08235
Size
100 µl
Form
Liquid
Description
Boster Bio Anti-AZI2 Antibody (Catalog # A08235). Tested in WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-AZI2 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A08235)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthesized peptide derived from N-terminal of human AZI2.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A08235 is reactive to AZI2 in Human, Mouse, Rat
Reconstitution
Calculated molecular weight
44935 MW
Background of Azi2
Adapter protein which binds TBK1 and IKBKE playing a role in antiviral innate immunity. Activates serine/threonine-protein kinase TBK1 and facilitates its oligomerization. Enhances the phosphorylation of NF-kappa-B p65 subunit RELA by TBK1. Promotes TBK1-induced as well as TNF-alpha or PMA-induced activation of NF-kappa-B. Participates in IFNB promoter activation via TICAM1.
Fujita F., Mol. Cell. Biol. 23:7780-7793(2003).
Ota T., Nat. Genet. 36:40-45(2004).
Sasai M., J. Immunol. 174:27-30(2005).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A08235 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blotting: 1:500~1:3000
Validation Images & Assay Conditions
Click image to see more details
Western blot analysis of extracts from HepG2 cells, using AZI2 antibody A08235.
Protein Target Info & Infographic
Gene/Protein Information For AZI2 (Source: Uniprot.org, NCBI)
Gene Name
AZI2
Full Name
5-azacytidine-induced protein 2
Weight
44935 MW
Alternative Names
5-azacytidine-induced protein 2;NF-kappa-B-activating kinase-associated protein 1;Nak-associated protein 1;TILP;AZI2;NAP1, TBKBP2; Azi2|AA410145, AZ, AZ2|5-azacytidine induced gene 2|5-azacytidine-induced protein 2|NF-kappa-B-activating kinase-associated protein 1|nak-associated protein 1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on AZI2, check out the AZI2 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for AZI2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-AZI2 Antibody (A08235)
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No publications found for A08235
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Customer Q&As
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6 Customer Q&As for Anti-AZI2 Antibody
Question
Does A08235 anti-AZI2 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-12-27
Answer
It shows on the product datasheet, A08235 anti-AZI2 antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-12-27
Question
I have attached the WB image, lot number and protocol we used for embryo hepatoma using anti-AZI2 antibody A08235. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-10-01
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-10-01
Question
I appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for embryo hepatoma using anti-AZI2 antibody A08235. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2017-09-13
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2017-09-13
Question
We are currently using anti-AZI2 antibody A08235 for human tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on primate tissues as well?
J. Singh
Verified customer
Asked: 2016-08-09
Answer
The anti-AZI2 antibody (A08235) has not been tested for cross reactivity specifically with primate tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in primate you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2016-08-09
Question
Will anti-AZI2 antibody A08235 work for WB with embryo hepatoma?
G. Thomas
Verified customer
Asked: 2016-03-03
Answer
According to the expression profile of embryo hepatoma, AZI2 is highly expressed in embryo hepatoma. So, it is likely that anti-AZI2 antibody A08235 will work for WB with embryo hepatoma.
Boster Scientific Support
Answered: 2016-03-03
Question
I was wanting to use your anti-AZI2 antibody for WB for human embryo hepatoma on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human embryo hepatoma identification?
K. Kulkarni
Verified customer
Asked: 2014-11-14
Answer
You can see on the product datasheet, A08235 anti-AZI2 antibody has been validated for WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in human embryo hepatoma in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2014-11-14