Click image to see more details
Product Info Summary
| SKU: | M00246-3 |
|---|---|
| Size: | 100ul |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Mouse |
| Application: | IF, IHC, WB |
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Product info
Product Name
Anti-Aurora A/B Kinase Aurkb Monoclonal Antibody
SKU/Catalog Number
M00246-3
Size
100ul
Description
Boster Bio Anti-Aurora A/B Kinase Aurkb Monoclonal Antibody catalog # M00246-3. Tested in IF, IHC, WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Aurora A/B Kinase Aurkb Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00246-3)
Host
Mouse
Contents
Purified antibody at 1mg/mL in 50% PBS, 50% glycerol plus 5mM NaN3
Clonality
Monoclonal
Immunogen
Full length recombinant human Aurora A protein expressed in and purified from E. coli.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M00246-3 is reactive to Aurkb in Human, Mouse, Rat
Reconstitution
Calculated molecular weight
39384 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00246-3 is guaranteed for IF, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:1,000. IF/ICC or IHC: 1:500-1:1,000.
Validation Images & Assay Conditions
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Western blot analysis of different cell lysates and recombinant protein solutions using mouse mAb to aurora A/B, M00246-3. Left: cells were treated with 100ng/mL of nocodazol, a microtubule depolymerizing agent which induces cells to halt at G2/M phase, for 6 hours prior western blotting: [1] protein standard, [2] HeLa, [3] canine A72 cells, [4] equine NBL6 cells, and [5] murine KR158 cells. Right: western blot of purified full length human recombinant aurora A, B, C as indicated. Bands at 46kDa and 38kDa correspond to aurora A and B proteins respectively, showing that M00246-3 antibody reacts with these to proteins but not aurora C.
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HeLa cell cultures were stained with M00246-3 antibody (green). The antibody stains spindle poles and mitotic spindles at anaphase and concentrates on the midbody between the two daughter cells during telophase. It is therefore a useful marker of dividing cells. Cells were counterstained with our chicken polyclonal antibody to vimentin
Protein Target Info & Infographic
Gene/Protein Information For Aurkb (Source: Uniprot.org, NCBI)
Gene Name
Aurkb
Full Name
Aurora kinase B
Weight
39384 MW
Superfamily
protein kinase superfamily
Alternative Names
Aurora kinase B;2.7.11.1;Aurora 1;Aurora- and IPL1-like midbody-associated protein 1;Aurora/IPL1-related kinase 2;ARK-2;Aurora-related kinase 2;STK-1;Serine/threonine-protein kinase 12;Serine/threonine-protein kinase 5;Serine/threonine-protein kinase aurora-B;Aurkb;Aik2, Aim1, Airk2, Ark2, Stk1, Stk12, Stk5; AURKB AIK2, AIM-1, AIM1, ARK-2, ARK2, AurB, IPL1, PPP1R48, STK-1, STK12, STK5, aurkb-sv1, aurkb-sv2 aurora kinase B aurora kinase B|aurora kinase B-Sv1|aurora kinase B-Sv2|aurora- and Ipl1-like midbody-associated protein 1|aurora-1|aurora-B|aurora-related kinase 2|aurora/IPL1-related kinase 2|protein phosphatase 1, regulatory subunit 48|serine/threonine kinase 12|serine/threonine-protein kinase 12|serine/threonine-protein kinase 5|serine/threonine-protein kinase aurora-B
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on Aurkb, check out the Aurkb Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for Aurkb: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Aurora A/B Kinase Aurkb Monoclonal Antibody (M00246-3)
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No publications found for M00246-3
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Customer Q&As
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16 Customer Q&As for Anti-Aurora A/B Kinase Aurkb Monoclonal Antibody
Question
We ordered your anti-Aurora A/B Kinase Monoclonal antibody for WB on cervix carcinoma a few years ago. I am using human, and I plan to use the antibody for IHC next. I am looking for examining cervix carcinoma as well as cervix carcinoma erythroleukemia in our next experiment. Could you please give me some suggestion on which antibody would work the best for IHC?
Verified Customer
Verified customer
Asked: 2020-03-31
Answer
I took a look at the website and datasheets of our anti-Aurora A/B Kinase Monoclonal antibody and it appears that M00246-3 has been tested on human in both WB and IHC. Thus M00246-3 should work for your application. Our Boster satisfaction guarantee will cover this product for IHC in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for IHC detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2020-03-31
Question
We have seen staining in rat lung. Any tips? Is anti-Aurora A/B Kinase Monoclonal antibody supposed to stain lung positively?
Verified Customer
Verified customer
Asked: 2020-03-25
Answer
From literature lung does express AURKB. From Uniprot.org, AURKB is expressed in oocyte, liver spleen, lung, lymph muscle, cervix carcinoma, cervix carcinoma erythroleukemia, among other tissues. Regarding which tissues have AURKB expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, Pubmed ID: 18669648, 18691976, 20068231
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Liver, and Spleen, Pubmed ID: 9858806
Lung, Lymph, and Muscle, Pubmed ID: 15489334
Boster Scientific Support
Answered: 2020-03-25
Question
Is a blocking peptide available for product anti-Aurora A/B Kinase Monoclonal antibody (M00246-3)?
Verified Customer
Verified customer
Asked: 2020-01-30
Answer
We do provide the blocking peptide for product anti-Aurora A/B Kinase Monoclonal antibody (M00246-3). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2020-01-30
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for cervix carcinoma erythroleukemia using anti-Aurora A/B Kinase Monoclonal antibody M00246-3. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-12-23
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-12-23
Question
My team were content with the WB result of your anti-Aurora A/B Kinase Monoclonal antibody. However we have seen positive staining in lymph muscle nucleus. chromosome. chromosome using this antibody. Is that expected? Could you tell me where is AURKB supposed to be expressed?
Verified Customer
Verified customer
Asked: 2019-12-05
Answer
From what I have seen in literature, lymph muscle does express AURKB. Generally AURKB expresses in nucleus. chromosome. chromosome, centromere. Regarding which tissues have AURKB expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, Pubmed ID: 18669648, 18691976, 20068231
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Liver, and Spleen, Pubmed ID: 9858806
Lung, Lymph, and Muscle, Pubmed ID: 15489334
Boster Scientific Support
Answered: 2019-12-05
Question
I am interested in to test anti-Aurora A/B Kinase Monoclonal antibody M00246-3 on human cervix carcinoma erythroleukemia for research purposes, then I may be interested in using anti-Aurora A/B Kinase Monoclonal antibody M00246-3 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-10-25
Answer
The products we sell, including anti-Aurora A/B Kinase Monoclonal antibody M00246-3, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-10-25
Question
I see that the anti-Aurora A/B Kinase Monoclonal antibody M00246-3 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-07-05
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2019-07-05
Question
I have attached the WB image, lot number and protocol we used for cervix carcinoma erythroleukemia using anti-Aurora A/B Kinase Monoclonal antibody M00246-3. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-05-30
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-05-30
Question
Is there a BSA free version of anti-Aurora A/B Kinase Monoclonal antibody M00246-3 available?
Verified Customer
Verified customer
Asked: 2019-04-08
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-Aurora A/B Kinase Monoclonal antibody M00246-3 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-04-08
Question
We are currently using anti-Aurora A/B Kinase Monoclonal antibody M00246-3 for mouse tissue, and we are content with the IF results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on monkey tissues as well?
Verified Customer
Verified customer
Asked: 2019-03-04
Answer
The anti-Aurora A/B Kinase Monoclonal antibody (M00246-3) has not been tested for cross reactivity specifically with monkey tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in monkey you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-03-04
Question
Is this M00246-3 anti-Aurora A/B Kinase Monoclonal antibody reactive to the isotypes of AURKB?
Verified Customer
Verified customer
Asked: 2019-02-11
Answer
The immunogen of M00246-3 anti-Aurora A/B Kinase Monoclonal antibody is Full length recombinant human Aurora A protein expressed in and purified from E. coli. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-02-11
Question
Does anti-Aurora A/B Kinase Monoclonal antibody M00246-3 work for WB with cervix carcinoma erythroleukemia?
Verified Customer
Verified customer
Asked: 2018-02-21
Answer
According to the expression profile of cervix carcinoma erythroleukemia, AURKB is highly expressed in cervix carcinoma erythroleukemia. So, it is likely that anti-Aurora A/B Kinase Monoclonal antibody M00246-3 will work for WB with cervix carcinoma erythroleukemia.
Boster Scientific Support
Answered: 2018-02-21
Question
I was wanting to use your anti-Aurora A/B Kinase Monoclonal antibody for WB for human cervix carcinoma erythroleukemia on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human cervix carcinoma erythroleukemia identification?
S. Miller
Verified customer
Asked: 2017-08-14
Answer
As indicated on the product datasheet, M00246-3 anti-Aurora A/B Kinase Monoclonal antibody has been tested for IF, IHC, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in human cervix carcinoma erythroleukemia in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2017-08-14
Question
My question regarding product M00246-3, anti-Aurora A/B Kinase Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
S. Miller
Verified customer
Asked: 2015-07-24
Answer
We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M00246-3 anti-Aurora A/B Kinase Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2015-07-24
Question
My question regards using your anti-Aurora A/B Kinase Monoclonal antibody for negative regulation of b cell apoptotic process studies. Has this antibody been tested with western blotting on dividing cells? We would like to see some validation images before ordering.
D. Collins
Verified customer
Asked: 2013-05-22
Answer
Thanks for your inquiry. This M00246-3 anti-Aurora A/B Kinase Monoclonal antibody is validated on canine a72 cells, equine nbl6 cells, murine kr158 cells, dividing cells. It is guaranteed to work for IF, IHC, WB in human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2013-05-22
Question
Does M00246-3 anti-Aurora A/B Kinase Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
M. Banerjee
Verified customer
Asked: 2013-01-29
Answer
It shows on the product datasheet, M00246-3 anti-Aurora A/B Kinase Monoclonal antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2013-01-29
