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Product Info Summary
| SKU: | M00246-2 |
|---|---|
| Size: | 100ul |
| Reactive Species: | Bovine, Equine, Human, Mouse, Pig, Rat |
| Host: | Mouse |
| Application: | IHC, WB |
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Product info
Product Name
Anti-Aurora A/B/C Kinase Aurkb Monoclonal Antibody
SKU/Catalog Number
M00246-2
Size
100ul
Description
Boster Bio Anti-Aurora A/B/C Kinase Aurkb Monoclonal Antibody catalog # M00246-2. Tested in IHC, WB applications. This antibody reacts with Bovine, Equine, Human, Mouse, Pig, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Aurora A/B/C Kinase Aurkb Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00246-2)
Host
Mouse
Contents
Purified antibody at 1mg/mL in 50% PBS, 50% glycerol plus 5mM NaN3
Clonality
Monoclonal
Immunogen
Full length recombinant human AURKA protein expressed from E. coli.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M00246-2 is reactive to Aurkb in Bovine, Equine, Human, Mouse, Pig, Rat
Reconstitution
Calculated molecular weight
39384 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00246-2 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:1,000. ICC/IF or IHC: 1:1,000-1:2,000.
Validation Images & Assay Conditions
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Left: Western blot analysis of M00246-2 in HeLa cells. Blot of HeLa cells treated with 100ng/ml nocodazole for 18 hours was probed with M00246-2. Nocodazole is a microtubule depolymerizating agent which induces cells to halt at the G2/M phase and also induces aurora kinase expression. The M00246-2 monoclonal binds strongly to bands at about 46kDa and 38kDa, corresponding to aurora A and aurora B. It also recognizes a weak band at 35kDa which is aurora C. Right: Blot of recombinant full length human aurora A, B and C proteins were probed with M00246-2. This antibody therefore reacts strongly with all three aurora kinases proteins.
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HeLa cell cultures were stained with M00246-2 antibody (green). Strong staining in spindle poles is seen in cells at anaphase and the antibody also stains the midbodies between daughter cells.
Protein Target Info & Infographic
Gene/Protein Information For Aurkb (Source: Uniprot.org, NCBI)
Gene Name
Aurkb
Full Name
Aurora kinase B
Weight
39384 MW
Superfamily
protein kinase superfamily
Alternative Names
Aurora kinase B;2.7.11.1;Aurora 1;Aurora- and IPL1-like midbody-associated protein 1;Aurora/IPL1-related kinase 2;ARK-2;Aurora-related kinase 2;STK-1;Serine/threonine-protein kinase 12;Serine/threonine-protein kinase 5;Serine/threonine-protein kinase aurora-B;Aurkb;Aik2, Aim1, Airk2, Ark2, Stk1, Stk12, Stk5; AURKB AIK2, AIM-1, AIM1, ARK-2, ARK2, AurB, IPL1, PPP1R48, STK-1, STK12, STK5, aurkb-sv1, aurkb-sv2 aurora kinase B aurora kinase B|aurora kinase B-Sv1|aurora kinase B-Sv2|aurora- and Ipl1-like midbody-associated protein 1|aurora-1|aurora-B|aurora-related kinase 2|aurora/IPL1-related kinase 2|protein phosphatase 1, regulatory subunit 48|serine/threonine kinase 12|serine/threonine-protein kinase 12|serine/threonine-protein kinase 5|serine/threonine-protein kinase aurora-B
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on Aurkb, check out the Aurkb Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for Aurkb: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Aurora A/B/C Kinase Aurkb Monoclonal Antibody (M00246-2)
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No publications found for M00246-2
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Customer Reviews
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Customer Q&As
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16 Customer Q&As for Anti-Aurora A/B/C Kinase Aurkb Monoclonal Antibody
Question
Our lab used your anti-Aurora A/B/C Kinase Monoclonal antibody for IHC on cervix carcinoma erythroleukemia in the past. I am using human, and We are going to use the antibody for WB next. you antibody examining cervix carcinoma erythroleukemia as well as liver spleen in our next experiment. Could you please give me some suggestion on which antibody would work the best for WB?
Verified Customer
Verified customer
Asked: 2020-02-12
Answer
I have checked the website and datasheets of our anti-Aurora A/B/C Kinase Monoclonal antibody and it seems that M00246-2 has been tested on human in both IHC and WB. Thus M00246-2 should work for your application. Our Boster satisfaction guarantee will cover this product for WB in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for WB detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2020-02-12
Question
Is a blocking peptide available for product anti-Aurora A/B/C Kinase Monoclonal antibody (M00246-2)?
Verified Customer
Verified customer
Asked: 2020-01-09
Answer
We do provide the blocking peptide for product anti-Aurora A/B/C Kinase Monoclonal antibody (M00246-2). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2020-01-09
Question
Thanks for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for lymph muscle using anti-Aurora A/B/C Kinase Monoclonal antibody M00246-2. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-10-31
Answer
Thanks for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-10-31
Question
Is this M00246-2 anti-Aurora A/B/C Kinase Monoclonal antibody reactive to the isotypes of AURKB?
Verified Customer
Verified customer
Asked: 2019-10-23
Answer
The immunogen of M00246-2 anti-Aurora A/B/C Kinase Monoclonal antibody is Full length recombinant human AURKA protein expressed from E. coli. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-10-23
Question
Do you have a BSA free version of anti-Aurora A/B/C Kinase Monoclonal antibody M00246-2 available?
Verified Customer
Verified customer
Asked: 2019-09-11
Answer
Thanks for your recent telephone inquiry. I can confirm that some lots of this anti-Aurora A/B/C Kinase Monoclonal antibody M00246-2 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-09-11
Question
Does M00246-2 anti-Aurora A/B/C Kinase Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-06-17
Answer
As indicated on the product datasheet, M00246-2 anti-Aurora A/B/C Kinase Monoclonal antibody as been tested on IHC. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-06-17
Question
I was wanting to use your anti-Aurora A/B/C Kinase Monoclonal antibody for IHC for equine lymph muscle on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for equine lymph muscle identification?
Verified Customer
Verified customer
Asked: 2019-06-13
Answer
It shows on the product datasheet, M00246-2 anti-Aurora A/B/C Kinase Monoclonal antibody has been validated for IHC, WB on bovine, equine, human, mouse, pig, rat tissues. We have an innovator award program that if you test this antibody and show it works in equine lymph muscle in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-06-13
Question
We are currently using anti-Aurora A/B/C Kinase Monoclonal antibody M00246-2 for pig tissue, and we are satisfied with the IHC results. The species of reactivity given in the datasheet says bovine, equine, human, mouse, pig, rat. Is it possible that the antibody can work on primate tissues as well?
Verified Customer
Verified customer
Asked: 2018-07-06
Answer
The anti-Aurora A/B/C Kinase Monoclonal antibody (M00246-2) has not been tested for cross reactivity specifically with primate tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in primate you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2018-07-06
Question
Does anti-Aurora A/B/C Kinase Monoclonal antibody M00246-2 work for IHC with lymph muscle?
Verified Customer
Verified customer
Asked: 2018-05-28
Answer
According to the expression profile of lymph muscle, AURKB is highly expressed in lymph muscle. So, it is likely that anti-Aurora A/B/C Kinase Monoclonal antibody M00246-2 will work for IHC with lymph muscle.
Boster Scientific Support
Answered: 2018-05-28
Question
Can you help my question with product M00246-2, anti-Aurora A/B/C Kinase Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
B. Parker
Verified customer
Asked: 2018-02-23
Answer
We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M00246-2 anti-Aurora A/B/C Kinase Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2018-02-23
Question
I would like to test anti-Aurora A/B/C Kinase Monoclonal antibody M00246-2 on equine lymph muscle for research purposes, then I may be interested in using anti-Aurora A/B/C Kinase Monoclonal antibody M00246-2 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2017-12-18
Answer
The products we sell, including anti-Aurora A/B/C Kinase Monoclonal antibody M00246-2, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2017-12-18
Question
I see that the anti-Aurora A/B/C Kinase Monoclonal antibody M00246-2 works with IHC, what is the protocol used to produce the result images on the product page?
T. Krishna
Verified customer
Asked: 2017-09-13
Answer
You can find protocols for IHC on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2017-09-13
Question
We were happy with the WB result of your anti-Aurora A/B/C Kinase Monoclonal antibody. However we have seen positive staining in lung centromere. using this antibody. Is that expected? Could you tell me where is AURKB supposed to be expressed?
Verified Customer
Verified customer
Asked: 2017-09-12
Answer
From literature, lung does express AURKB. Generally AURKB expresses in nucleus. chromosome. chromosome, centromere. Regarding which tissues have AURKB expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, Pubmed ID: 18669648, 18691976, 20068231
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Liver, and Spleen, Pubmed ID: 9858806
Lung, Lymph, and Muscle, Pubmed ID: 15489334
Boster Scientific Support
Answered: 2017-09-12
Question
We have seen staining in equine liver spleen. What should we do? Is anti-Aurora A/B/C Kinase Monoclonal antibody supposed to stain liver spleen positively?
D. Parker
Verified customer
Asked: 2016-03-11
Answer
Based on literature liver spleen does express AURKB. Based on Uniprot.org, AURKB is expressed in oocyte, liver spleen, lung, lymph muscle, cervix carcinoma, cervix carcinoma erythroleukemia, among other tissues. Regarding which tissues have AURKB expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, Pubmed ID: 18669648, 18691976, 20068231
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Liver, and Spleen, Pubmed ID: 9858806
Lung, Lymph, and Muscle, Pubmed ID: 15489334
Boster Scientific Support
Answered: 2016-03-11
Question
My question regards using your anti-Aurora A/B/C Kinase Monoclonal antibody for histone h3-s28 phosphorylation studies. Has this antibody been tested with western blotting on hela cells? We would like to see some validation images before ordering.
L. Carter
Verified customer
Asked: 2015-11-19
Answer
I appreciate your inquiry. This M00246-2 anti-Aurora A/B/C Kinase Monoclonal antibody is validated on hela cells. It is guaranteed to work for IHC, WB in bovine, equine, human, mouse, pig, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2015-11-19
Question
Here is the WB image, lot number and protocol we used for lymph muscle using anti-Aurora A/B/C Kinase Monoclonal antibody M00246-2. Please let me know if you require anything else.
C. Johnson
Verified customer
Asked: 2013-07-01
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2013-07-01
