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Product Info Summary
| SKU: | A00203-2 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, Flow Cytometry, IHC, WB |
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Product info
Product Name
Anti-ATRX Antibody Picoband®
SKU/Catalog Number
A00203-2
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-ATRX Antibody Picoband® catalog # A00203-2. Tested in ELISA, Flow Cytometry, IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-ATRX Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00203-2)
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human ATRX recombinant protein (Position: E8-Q289).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A00203-2 is reactive to ATRX in Human, Mouse, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
282 kDa
Calculated molecular weight
282.586kDa
Background of ATRX
Transcriptional regulator ATRX also known as ATP-dependent helicase ATRX, X-linked helicase II, or X-linked nuclear protein (XNP) is a protein that in humans is encoded by the ATRX gene. It is mapped to Xq21.1. The protein encoded by this gene contains an ATPase/helicase domain, and thus it belongs to the SWI/SNF family of chromatin remodeling proteins. This protein is found to undergo cell cycle-dependent phosphorylation, which regulates its nuclear matrix and chromatin association, and suggests its involvement in the gene regulation at interphase and chromosomal segregation in mitosis. Mutations in this gene are associated with X-linked syndromes exhibiting cognitive disabilities as well as alpha-thalassemia (ATRX) syndrome. These mutations have been shown to cause diverse changes in the pattern of DNA methylation, which may provide a link between chromatin remodeling, DNA methylation, and gene expression in developmental processes. Multiple alternatively spliced transcript variants encoding distinct isoforms have been reported.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A00203-2 is guaranteed for ELISA, Flow Cytometry, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5μg/ml, Human
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Mouse, Rat, By Heat
Flow Cytometry (Fixed), 1-3μg/1x106 cells, Human
ELISA, 0.1-0.5μg/ml, -
Positive Control
WB: human A54 whole cell, human Hela whole cell
IHC: mouse testis tissue, rat testis tissue, rat testis tissue, human mammary cancer tissue, human mammary cancer tissue, rat small intestine tissue, rat small intestine tissue
FCM: A549 cell
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of ATRX using anti-ATRX antibody (A00203-2).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human A54 whole cell lysates,
Lane 2: human Hela whole cell lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ATRX antigen affinity purified polyclonal antibody (Catalog # A00203-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ATRX at approximately 282KD. The expected band size for ATRX is at 282KD.
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Figure 2. IHC analysis of ATRX using anti-ATRX antibody (A00203-2).
ATRX was detected in paraffin-embedded section of mouse testis tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-ATRX Antibody (A00203-2) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Click image to see more details
Figure 3. IHC analysis of ATRX using anti-ATRX antibody (A00203-2).
ATRX was detected in paraffin-embedded section of rat testis tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-ATRX Antibody (A00203-2) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Click image to see more details
Figure 4. IHC analysis of ATRX using anti-ATRX antibody (A00203-2).
ATRX was detected in paraffin-embedded section of rat testis tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-ATRX Antibody (A00203-2) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Click image to see more details
Figure 5. IHC analysis of ATRX using anti-ATRX antibody (A00203-2).
ATRX was detected in paraffin-embedded section of human mammary cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-ATRX Antibody (A00203-2) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Click image to see more details
Figure 6. IHC analysis of ATRX using anti-ATRX antibody (A00203-2).
ATRX was detected in paraffin-embedded section of human mammary cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-ATRX Antibody (A00203-2) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Click image to see more details
Figure 7. IHC analysis of ATRX using anti-ATRX antibody (A00203-2).
ATRX was detected in paraffin-embedded section of rat small intestine tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-ATRX Antibody (A00203-2) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Click image to see more details
Figure 8. IHC analysis of ATRX using anti-ATRX antibody (A00203-2).
ATRX was detected in paraffin-embedded section of rat small intestine tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-ATRX Antibody (A00203-2) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Click image to see more details
Figure 9. Flow Cytometry analysis of A549 cells using anti-ATRX antibody (A00203-2).
Overlay histogram showing A549 cells stained with A00203-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-ATRX Antibody (A00203-2,1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Protein Target Info & Infographic
Gene/Protein Information For ATRX (Source: Uniprot.org, NCBI)
Gene Name
ATRX
Full Name
Transcriptional regulator ATRX
Weight
282.586kDa
Superfamily
SNF2/RAD54 helicase family
Alternative Names
Transcriptional regulator ATRX; ATP-dependent helicase ATRX; X-linked helicase II; X-linked nuclear protein; XNP; Znf-HX; ATRX; RAD54L, XH2 ATRX JMS, MRX52, RAD54, RAD54L, XH2, XNP, ZNF-HX ATRX chromatin remodeler transcriptional regulator ATRX|ATP-dependent helicase ATRX|X-linked helicase II|X-linked nuclear protein|alpha thalassemia/mental retardation syndrome X-linked (RAD54 homolog, S. cerevisiae)
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on ATRX, check out the ATRX Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for ATRX: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-ATRX Antibody Picoband® (A00203-2)
Hello CJ!
A00203-2 has been cited in 1 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Co-occurrence of ATRX and IDH Mutations Identify Subgroup of Glioma Patients for Better Survival. Trivedi Trupti,Panchal Kinjal,Bhalala Neha,Jyotishi Charmi,Trivedi Priti,Panchal Harsha,Modi Nikhi
Species: Human
A00203-2 usage in article: APP:IHC, SAMPLE:GLIOMA TISSUE, DILUTION:1:100
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Customer Q&As
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6 Customer Q&As for Anti-ATRX Antibody Picoband®
Question
Nuclear staining is expected and seen in the mouse/rat sections but the breast cancer tissue looks to have cytoplasmic staining and no nuclear staining.Would you happen to have additional IHC-P staining of human tissue for A00203-2?
Verified customer
Asked: 2020-10-01
Answer
Although nuclear staining on the breast cancer tissue was not obvious, weak nuclear staining could be observed. It is mainly cytoplasmic staining according to our in house test using the Anti-ATRX Antibody Picoband™ (A00203-2). Our lab hasn't got additional IHC-P staining of human tissues.
Boster Scientific Support
Answered: 2020-10-09
Question
We have been able to see staining in mouse liver. What should we do? Is anti-ATRX antibody supposed to stain liver positively?
Verified Customer
Verified customer
Asked: 2020-03-04
Answer
Based on literature liver does express ATRX. Based on Uniprot.org, ATRX is expressed in frontal cortex, brain, cervix carcinoma, embryonic kidney, leukemic t-cell, cervix carcinoma erythroleukemia, liver, among other tissues. Regarding which tissues have ATRX expression, here are a few articles citing expression in various tissues:
Brain, Pubmed ID: 15772651
Cervix carcinoma, Pubmed ID: 17081983, 18669648, 20068231
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Embryonic kidney, Pubmed ID: 17525332
Leukemic T-cell, Pubmed ID: 19690332
Liver, Pubmed ID: 24275569
Boster Scientific Support
Answered: 2020-03-04
Question
My question regards using your anti-ATRX antibody for positive regulation of telomeric rna transcription from rna pol ii promoter studies. Has this antibody been tested with western blotting on hela whole cell lysates? We would like to see some validation images before ordering.
Verified Customer
Verified customer
Asked: 2020-01-22
Answer
Thank you for your inquiry. This A00203-2 anti-ATRX antibody is tested on human hela, a549 cells, hela whole cell lysates. It is guaranteed to work for ELISA, Flow Cytometry, IHC-P, WB in human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2020-01-22
Question
My colleagues were happy with the WB result of your anti-ATRX antibody. However we have been able to see positive staining in brain pml using this antibody. Is that expected? Could you tell me where is ATRX supposed to be expressed?
Verified Customer
Verified customer
Asked: 2018-10-01
Answer
From literature, brain does express ATRX. Generally ATRX expresses in nucleus. chromosome, telomere. nucleus, pml. Regarding which tissues have ATRX expression, here are a few articles citing expression in various tissues:
Brain, Pubmed ID: 15772651
Cervix carcinoma, Pubmed ID: 17081983, 18669648, 20068231
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Embryonic kidney, Pubmed ID: 17525332
Leukemic T-cell, Pubmed ID: 19690332
Liver, Pubmed ID: 24275569
Boster Scientific Support
Answered: 2018-10-01
Question
We are currently using anti-ATRX antibody A00203-2 for rat tissue, and we are content with the Flow Cytometry results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on horse tissues as well?
A. Gonzalez
Verified customer
Asked: 2017-10-26
Answer
The anti-ATRX antibody (A00203-2) has not been validated for cross reactivity specifically with horse tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in horse you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2017-10-26
Question
We ordered your anti-ATRX antibody for ELISA on leukemic t-cell a few months ago. I am using mouse, and I plan to use the antibody for WB next. My question regards examining leukemic t-cell as well as brain in our next experiment. Could you please give me some suggestion on which antibody would work the best for WB?
K. Taylor
Verified customer
Asked: 2014-02-04
Answer
I viewed the website and datasheets of our anti-ATRX antibody and it seems that A00203-2 has been validated on mouse in both ELISA and WB. Thus A00203-2 should work for your application. Our Boster satisfaction guarantee will cover this product for WB in mouse even if the specific tissue type has not been validated. We do have a comprehensive range of products for WB detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2014-02-04
