Product Info Summary
SKU: | RP1055 |
---|---|
Size: | 100 μg/vial |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | IHC, WB |
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Product info
Product Name
Anti-SERCA3 ATPase/ATP2A3 Antibody
View all SERCA3 ATPase Antibodies
SKU/Catalog Number
RP1055
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-SERCA3 ATPase/ATP2A3 Antibody catalog # RP1055. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-SERCA3 ATPase/ATP2A3 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # RP1055)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Standard Protein
A synthetic peptide corresponding to a sequence at the N-terminus of human ATP2A3, different from the related mouse sequence by five amino acids, and from the related rat sequence by six amino acids.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
RP1055 is reactive to ATP2A3 in Human, Mouse, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
114 kDa
Calculated molecular weight
113977 MW
Background of SERCA3 ATPase
Sarcoplasmic/endoplasmic reticulum calcium ATPase 3, also known as SERCA3, is anenzyme that in humans is encoded by the ATP2A3 gene. It is mapped to 17p13.2. This gene encodes one of the SERCA Ca2+-ATPases, which are intracellular pumps located in the sarcoplasmic or endoplasmic reticula of cells. ATP2A3 expression was originally described as non-muscular, but was recently observed in cardiomyocyte. What’s more, the expression of ATP2A3 was significantly reduced or lost in colon carcinomas compared with normal colonic epithelial cells. This enzyme catalyzes the hydrolysis of ATP coupled with the translocation of calcium from the cytosol to the sarcoplasmic reticulum lumen, and is involved in calcium sequestration associated with muscular excitation and contraction.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
RP1055 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Mouse, Rat, Human, By Heat
Western blot, 0.1-0.5μg/ml, Mouse, Rat, Human
Positive Control
WB: Rat Skeletal Muscle Tissue, Mouse Skeletal Muscle Tissue
IHC: Mouse Thymus Tissue, Rat Thymus Tissue
Validation Images & Assay Conditions
Click image to see more details
Anti-ATP2A3 antibody, RP1055, Western blotting
All lanes: Anti ATP2A3 (RP1055) at 0.5ug/ml
Lane 1: Rat Skeletal Muscle Tissue Lysate at 50ug
Lane 2: Mouse Skeletal Muscle Tissue Lysate at 50ug
Predicted bind size: 114KD
Observed bind size: 114KD
Click image to see more details
Anti-ATP2A3 antibody, RP1055, IHC(P)
IHC(P): Mouse Thymus Tissue
Click image to see more details
Anti-ATP2A3 antibody, RP1055,IHC(P)
IHC(P): Rat Thymus Tissue
Protein Target Info & Infographic
Gene/Protein Information For ATP2A3 (Source: Uniprot.org, NCBI)
Gene Name
ATP2A3
Full Name
Sarcoplasmic/endoplasmic reticulum calcium ATPase 3
Weight
113977 MW
Superfamily
cation transport ATPase (P-type) (TC 3.A.3) family
Alternative Names
adenosine triphosphatase, calcium; ATPase, Ca(2+)-transporting, ubiquitous; ATPase, Ca++ transporting, ubiquitous; Calcium pump 3; EC 3.6.3; EC 3.6.3.8; sarco/endoplasmic reticulum Ca2+ ATPase; sarco/endoplasmic reticulum Ca2+ -ATPase; sarcoplasmic/endoplasmic reticulum calcium ATPase 3; SERCA3calcium-translocating P-type ATPase; SR Ca(2+)-ATPase 3 ATP2A3 SERCA3 ATPase sarcoplasmic/endoplasmic reticulum Ca2+ transporting 3 sarcoplasmic/endoplasmic reticulum calcium ATPase 3|ATPase, Ca(2+)-transporting, ubiquitous|ATPase, Ca++ transporting, ubiquitous|SR Ca(2+)-ATPase 3|adenosine triphosphatase, calcium|calcium pump 3|calcium-translocating P-type ATPase|sarco/endoplasmic reticulum Ca2+ -ATPase
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on ATP2A3, check out the ATP2A3 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for ATP2A3: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-SERCA3 ATPase/ATP2A3 Antibody (RP1055)
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Customer Q&As
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5 Customer Q&As for Anti-SERCA3 ATPase/ATP2A3 Antibody
Question
I have a question about product RP1055, anti-SERCA3 ATPase/ATP2A3 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2020-05-06
Answer
It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free RP1055 anti-SERCA3 ATPase/ATP2A3 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2020-05-06
Question
I see that the anti-SERCA3 ATPase/ATP2A3 antibody RP1055 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2020-04-10
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2020-04-10
Question
Would RP1055 anti-SERCA3 ATPase/ATP2A3 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2020-04-09
Answer
It shows on the product datasheet, RP1055 anti-SERCA3 ATPase/ATP2A3 antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2020-04-09
Question
Will anti-SERCA3 ATPase/ATP2A3 antibody RP1055 work on horse WB with erythroleukemia?
J. Taylor
Verified customer
Asked: 2017-08-04
Answer
Our lab technicians have not validated anti-SERCA3 ATPase/ATP2A3 antibody RP1055 on horse. You can run a BLAST between horse and the immunogen sequence of anti-SERCA3 ATPase/ATP2A3 antibody RP1055 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated horse samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in horse erythroleukemia in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2017-08-04
Question
We are currently using anti-SERCA3 ATPase/ATP2A3 antibody RP1055 for rat tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on pig tissues as well?
R. Collins
Verified customer
Asked: 2015-12-18
Answer
The anti-SERCA3 ATPase/ATP2A3 antibody (RP1055) has not been tested for cross reactivity specifically with pig tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in pig you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2015-12-18