Product Info Summary
SKU: | M00014 |
---|---|
Size: | 100 μl |
Reactive Species: | Human |
Host: | Rabbit |
Application: | Flow Cytometry, IF, IHC, ICC, WB |
Customers Who Bought This Also Bought
Product info
Product Name
Anti-ATM Monoclonal Antibody
SKU/Catalog Number
M00014
BM4091 is an alternative SKU for this antibody, used in previous lots.
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-ATM Monoclonal Antibody catalog # M00014. Tested in WB, IHC, ICC/IF, Flow Cytometry applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-ATM Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00014)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
BCF-1
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human ATM
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M00014 is reactive to ATM in Human
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
351 kDa
Calculated molecular weight
88068 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00014 is guaranteed for Flow Cytometry, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:50-1:100
ICC/IF 1:50-1:200
FC 1:50
Positive Control
WB: human HepG2 whole cell, human 293T whole cell
IHC: human breast cancer tissue
ICC/IF: Hela cell
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of ATM using anti-ATM antibody (M00014).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human HepG2 whole cell lysates,
Lane 2: human 293T whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ATM antigen affinity purified polyclonal antibody (Catalog # M00014) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ATM at approximately 351 kDa. The expected band size for ATM is at 351 kDa.
Click image to see more details
Immunohistochemical analysis of paraffin-embedded human breast cancer, using ATM Antibody.
Click image to see more details
Immunofluorescent analysis of Hela cells, using ATM Antibody.
Protein Target Info & Infographic
Gene/Protein Information For ATM (Source: Uniprot.org, NCBI)
Gene Name
ATM
Full Name
Serine-protein kinase ATM
Weight
88068 MW
Superfamily
PI3/PI4-kinase family
Alternative Names
Signal transducer and activator of transcription 3;Acute-phase response factor;STAT3;APRF; ATM AT1, ATA, ATC, ATD, ATDC, ATE, TEL1, TELO1 ATM serine/threonine kinase serine-protein kinase ATM|A-T mutated|AT mutated|TEL1, telomere maintenance 1, homolog|ataxia telangiectasia mutated
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on ATM, check out the ATM Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for ATM: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-ATM Monoclonal Antibody (M00014)
Hello CJ!
No publications found for M00014
*Do you have publications using this product? Share with us and receive a reward. Ask us for more details.
Recommended Resources
Here are featured tools and databases that you might find useful.
- Boster's Pathways Library
- Protein Databases
- Bioscience Research Protocol Resources
- Data Processing & Analysis Software
- Photo Editing Software
- Scientific Literature Resources
- Research Paper Management Tools
- Molecular Biology Software
- Primer Design Tools
- Bioinformatics Tools
- Phylogenetic Tree Analysis
Customer Reviews
Have you used Anti-ATM Monoclonal Antibody?
Submit a review and receive an Amazon gift card.
- $30 for a review with an image
0 Reviews For Anti-ATM Monoclonal Antibody
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question
16 Customer Q&As for Anti-ATM Monoclonal Antibody
Question
I have attached the WB image, lot number and protocol we used for fibroblast using anti-ATM Monoclonal antibody M00014. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2020-03-23
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-03-23
Question
We have observed staining in human cervix carcinoma. Any tips? Is anti-ATM Monoclonal antibody supposed to stain cervix carcinoma positively?
Verified Customer
Verified customer
Asked: 2020-03-11
Answer
From literature cervix carcinoma does express ATM. From Uniprot.org, ATM is expressed in corpus callosum, fibroblast, brain, embryonic kidney, cervix carcinoma, cervix carcinoma erythroleukemia, liver, among other tissues. Regarding which tissues have ATM expression, here are a few articles citing expression in various tissues:
Brain, Pubmed ID: 15489334
Cervix carcinoma, Pubmed ID: 18691976
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Embryonic kidney, Pubmed ID: 17525332
Fibroblast, Pubmed ID: 7792600
Liver, Pubmed ID: 24275569
Boster Scientific Support
Answered: 2020-03-11
Question
Does anti-ATM Monoclonal antibody M00014 work for IHC with fibroblast?
Verified Customer
Verified customer
Asked: 2020-03-05
Answer
According to the expression profile of fibroblast, ATM is highly expressed in fibroblast. So, it is likely that anti-ATM Monoclonal antibody M00014 will work for IHC with fibroblast.
Boster Scientific Support
Answered: 2020-03-05
Question
I would like to test anti-ATM Monoclonal antibody M00014 on human fibroblast for research purposes, then I may be interested in using anti-ATM Monoclonal antibody M00014 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2020-01-15
Answer
The products we sell, including anti-ATM Monoclonal antibody M00014, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2020-01-15
Question
My colleagues were happy with the WB result of your anti-ATM Monoclonal antibody. However we have been able to see positive staining in corpus callosum nucleus using this antibody. Is that expected? Could you tell me where is ATM supposed to be expressed?
Verified Customer
Verified customer
Asked: 2019-12-25
Answer
According to literature, corpus callosum does express ATM. Generally ATM expresses in nucleus. Regarding which tissues have ATM expression, here are a few articles citing expression in various tissues:
Brain, Pubmed ID: 15489334
Cervix carcinoma, Pubmed ID: 18691976
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Embryonic kidney, Pubmed ID: 17525332
Fibroblast, Pubmed ID: 7792600
Liver, Pubmed ID: 24275569
Boster Scientific Support
Answered: 2019-12-25
Question
Do you have a BSA free version of anti-ATM Monoclonal antibody M00014 available?
Verified Customer
Verified customer
Asked: 2019-12-10
Answer
I appreciate your recent telephone inquiry. I can confirm that some lots of this anti-ATM Monoclonal antibody M00014 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-12-10
Question
We are currently using anti-ATM Monoclonal antibody M00014 for human tissue, and we are content with the IF results. The species of reactivity given in the datasheet says human. Is it true that the antibody can work on horse tissues as well?
Verified Customer
Verified customer
Asked: 2019-09-09
Answer
The anti-ATM Monoclonal antibody (M00014) has not been tested for cross reactivity specifically with horse tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in horse you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-09-09
Question
We ordered your anti-ATM Monoclonal antibody for IHC on liver in a previous experiment. I am using human, and We intend to use the antibody for ICC next. We are interested in examining liver as well as brain in our next experiment. Could you please give me some suggestion on which antibody would work the best for ICC?
Verified Customer
Verified customer
Asked: 2019-08-19
Answer
I have checked the website and datasheets of our anti-ATM Monoclonal antibody and it appears that M00014 has been validated on human in both IHC and ICC. Thus M00014 should work for your application. Our Boster satisfaction guarantee will cover this product for ICC in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for ICC detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2019-08-19
Question
My question regarding product M00014, anti-ATM Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-07-29
Answer
We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M00014 anti-ATM Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-07-29
Question
I was wanting to use your anti-ATM Monoclonal antibody for IHC for human fibroblast on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human fibroblast identification?
Verified Customer
Verified customer
Asked: 2019-07-25
Answer
It shows on the product datasheet, M00014 anti-ATM Monoclonal antibody has been validated for Flow Cytometry, IF, IHC, ICC, WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human fibroblast in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-07-25
Question
Is this M00014 anti-ATM Monoclonal antibody reactive to the isotypes of ATM?
Verified Customer
Verified customer
Asked: 2018-11-09
Answer
The immunogen of M00014 anti-ATM Monoclonal antibody is A synthesized peptide derived from human ATM. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2018-11-09
Question
I am interested in using your anti-ATM Monoclonal antibody for replicative senescence studies. Has this antibody been tested with western blotting on hela cells? We would like to see some validation images before ordering.
Verified Customer
Verified customer
Asked: 2018-06-18
Answer
We appreciate your inquiry. This M00014 anti-ATM Monoclonal antibody is tested on hela cells. It is guaranteed to work for Flow Cytometry, IF, IHC, ICC, WB in human. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2018-06-18
Question
Would M00014 anti-ATM Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2018-02-27
Answer
It shows on the product datasheet, M00014 anti-ATM Monoclonal antibody as been validated on IHC. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2018-02-27
Question
I see that the anti-ATM Monoclonal antibody M00014 works with IHC, what is the protocol used to produce the result images on the product page?
C. Thomas
Verified customer
Asked: 2017-01-27
Answer
You can find protocols for IHC on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2017-01-27
Question
Is a blocking peptide available for product anti-ATM Monoclonal antibody (M00014)?
K. Carter
Verified customer
Asked: 2015-07-10
Answer
We do provide the blocking peptide for product anti-ATM Monoclonal antibody (M00014). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2015-07-10
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for fibroblast using anti-ATM Monoclonal antibody M00014. Let me know if you need anything else.
P. Carter
Verified customer
Asked: 2013-09-20
Answer
I appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2013-09-20