Product Info Summary
SKU: | M00014-1 |
---|---|
Size: | 100 μl |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | Flow Cytometry, IP, IF, ICC, WB |
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Product info
Product Name
Anti-ATM Monoclonal Antibody
SKU/Catalog Number
M00014-1
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-ATM Monoclonal Antibody catalog # M00014-1. Tested in WB, ICC/IF, IP, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-ATM Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00014-1)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
AECA-1
Isotype
Rabbit IgG
Standard Protein
A synthesized peptide derived from human ATM Serine/threonine protein kinase which activates checkpoint signaling upon double strand breaks (DSBs), apoptosis and genotoxic stresses such as ionizing ultraviolet A light (UVA), thereby acting as a DNA damage sensor.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M00014-1 is reactive to ATM in Human, Mouse, Rat
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
127 kDa
Calculated molecular weight
88068 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00014-1 is guaranteed for Flow Cytometry, IP, IF, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
ICC/IF 1:50-1:200
IP 1:80
FC 1:100
Positive Control
WB: 293 cell
Validation Images & Assay Conditions
Click image to see more details
Western blot analysis of ATM expression in 293 cell lysate.
Protein Target Info & Infographic
Gene/Protein Information For ATM (Source: Uniprot.org, NCBI)
Gene Name
ATM
Full Name
Serine-protein kinase ATM
Weight
88068 MW
Superfamily
PI3/PI4-kinase family
Alternative Names
AT mutated; A-T mutated; AT1; ATA; ataxia telangiectasia mutated (includes complementation groups A, C and D); ataxia telangiectasia mutatedATD; ATC; ATDC; ATE; ATM; DKFZp781A0353; EC 2.7.11.1; MGC74674; serine-protein kinase ATM; TEL1; TEL1, telomere maintenance 1, homolog; TELO1; TPLL ATM AT1, ATA, ATC, ATD, ATDC, ATE, TEL1, TELO1 ATM serine/threonine kinase serine-protein kinase ATM|A-T mutated|AT mutated|TEL1, telomere maintenance 1, homolog|ataxia telangiectasia mutated
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on ATM, check out the ATM Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for ATM: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-ATM Monoclonal Antibody (M00014-1)
Hello CJ!
M00014-1 has been cited in 1 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Wang P,Wang C,Liu C. Antitumor effects of dioscin in A431 cells via adjusting ATM/p53-mediated cell apoptosis, DNA damage and migration. Oncol Lett. 2021 Jan;21(1):59.doi:10.3892/ol.2020.12321.Epub 2020 Nov 19.PMID:33281970;PMCID:PMC7709553.
Species: Human
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Customer Reviews
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Customer Q&As
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15 Customer Q&As for Anti-ATM Monoclonal Antibody
Question
We are currently using anti-ATM Monoclonal antibody M00014-1 for rat tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on monkey tissues as well?
Verified Customer
Verified customer
Asked: 2020-03-04
Answer
The anti-ATM Monoclonal antibody (M00014-1) has not been tested for cross reactivity specifically with monkey tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in monkey you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-03-04
Question
We have seen staining in human embryonic kidney. Any tips? Is anti-ATM Monoclonal antibody supposed to stain embryonic kidney positively?
Verified Customer
Verified customer
Asked: 2020-02-21
Answer
Based on literature embryonic kidney does express ATM. Based on Uniprot.org, ATM is expressed in corpus callosum, fibroblast, brain, embryonic kidney, cervix carcinoma, cervix carcinoma erythroleukemia, liver, among other tissues. Regarding which tissues have ATM expression, here are a few articles citing expression in various tissues:
Brain, Pubmed ID: 15489334
Cervix carcinoma, Pubmed ID: 18691976
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Embryonic kidney, Pubmed ID: 17525332
Fibroblast, Pubmed ID: 7792600
Liver, Pubmed ID: 24275569
Boster Scientific Support
Answered: 2020-02-21
Question
We ordered your anti-ATM Monoclonal antibody for WB on corpus callosum a few years ago. I am using mouse, and We intend to use the antibody for IF next. My lab would like examining corpus callosum as well as brain in our next experiment. Could give a recommendation on which antibody would work the best for IF?
Verified Customer
Verified customer
Asked: 2020-01-22
Answer
I have checked the website and datasheets of our anti-ATM Monoclonal antibody and it seems that M00014-1 has been validated on mouse in both WB and IF. Thus M00014-1 should work for your application. Our Boster satisfaction guarantee will cover this product for IF in mouse even if the specific tissue type has not been validated. We do have a comprehensive range of products for IF detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2020-01-22
Question
I have a question about product M00014-1, anti-ATM Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-07-15
Answer
We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M00014-1 anti-ATM Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-07-15
Question
Is this M00014-1 anti-ATM Monoclonal antibody reactive to the isotypes of ATM?
Verified Customer
Verified customer
Asked: 2019-07-05
Answer
The immunogen of M00014-1 anti-ATM Monoclonal antibody is A synthesized peptide derived from human ATM Serine/threonine protein kinase which activates checkpoint signaling upon double strand breaks (DSBs), apoptosis and genotoxic stresses such as ionizing ultraviolet A light (UVA), thereby acting as a DNA damage sensor. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-07-05
Question
See below the WB image, lot number and protocol we used for liver using anti-ATM Monoclonal antibody M00014-1. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-06-12
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-06-12
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for liver using anti-ATM Monoclonal antibody M00014-1. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2018-10-01
Answer
Thank you for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-10-01
Question
I was wanting to use your anti-ATM Monoclonal antibody for IF for mouse liver on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for mouse liver identification?
Verified Customer
Verified customer
Asked: 2018-08-13
Answer
As indicated on the product datasheet, M00014-1 anti-ATM Monoclonal antibody has been validated for IP, IF, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in mouse liver in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2018-08-13
Question
Does M00014-1 anti-ATM Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2018-02-13
Answer
It shows on the product datasheet, M00014-1 anti-ATM Monoclonal antibody as been validated on IF. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2018-02-13
Question
Is there a BSA free version of anti-ATM Monoclonal antibody M00014-1 available?
Verified Customer
Verified customer
Asked: 2017-12-08
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-ATM Monoclonal antibody M00014-1 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2017-12-08
Question
I see that the anti-ATM Monoclonal antibody M00014-1 works with IF, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2017-08-10
Answer
You can find protocols for IF on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2017-08-10
Question
Would anti-ATM Monoclonal antibody M00014-1 work for IF with liver?
A. Miller
Verified customer
Asked: 2017-04-04
Answer
According to the expression profile of liver, ATM is highly expressed in liver. So, it is likely that anti-ATM Monoclonal antibody M00014-1 will work for IF with liver.
Boster Scientific Support
Answered: 2017-04-04
Question
We were happy with the WB result of your anti-ATM Monoclonal antibody. However we have been able to see positive staining in cervix carcinoma erythroleukemia nucleus using this antibody. Is that expected? Could you tell me where is ATM supposed to be expressed?
R. Krishna
Verified customer
Asked: 2015-02-13
Answer
From literature, cervix carcinoma erythroleukemia does express ATM. Generally ATM expresses in nucleus. Regarding which tissues have ATM expression, here are a few articles citing expression in various tissues:
Brain, Pubmed ID: 15489334
Cervix carcinoma, Pubmed ID: 18691976
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Embryonic kidney, Pubmed ID: 17525332
Fibroblast, Pubmed ID: 7792600
Liver, Pubmed ID: 24275569
Boster Scientific Support
Answered: 2015-02-13
Question
Is a blocking peptide available for product anti-ATM Monoclonal antibody (M00014-1)?
J. Krishna
Verified customer
Asked: 2014-03-24
Answer
We do provide the blocking peptide for product anti-ATM Monoclonal antibody (M00014-1). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2014-03-24
Question
I would like to test anti-ATM Monoclonal antibody M00014-1 on mouse liver for research purposes, then I may be interested in using anti-ATM Monoclonal antibody M00014-1 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
J. Huang
Verified customer
Asked: 2013-04-16
Answer
The products we sell, including anti-ATM Monoclonal antibody M00014-1, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2013-04-16