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Product Info Summary
| SKU: | PA1697 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Mouse, Rat |
| Host: | Rabbit |
| Application: | IHC, WB |
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Product info
Product Name
Anti-DNA repair protein XRCC3 XRCC3 Antibody Picoband®
SKU/Catalog Number
PA1697
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-DNA repair protein XRCC3 XRCC3 Antibody catalog # PA1697. Tested in IHC, WB applications. This antibody reacts with Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-DNA repair protein XRCC3 XRCC3 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1697)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of mouse XRCC3, identical to the related rat sequence.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PA1697 is reactive to Xrcc3 in Mouse, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
38 kDa
Calculated molecular weight
38446 MW
Background of XRCC3
XRCC3 (X-RAY REPAIR, COMPLEMENTING DEFECTIVE, IN CHINESE HAMSTER, 3) is a DNA repair protein that in humans, is encoded by the XRCC3 gene. The XRCC3 is a member of the RecA/Rad51-related protein family that participates in homologous recombination to maintain chromosome stability and repair DNA damage. XRCC3 interacts directly with RAD51 and may cooperate with RAD51 during recombinational repair. XRCC3 function is not limited to HR initiation, but extends to later stages in formation and resolution of HR intermediates, possibly by stabilizing heteroduplex DNA. The gene functionally complements Chinese hamster irs1SF, a repair-deficient mutant that exhibits hypersensitivity to a number of different DNA-damaging agents and is chromosomally unstable. A rare microsatellite polymorphism in this gene is associated with cancer in patients of varying radiosensitivity.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PA1697 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Rat, Mouse, By Heat
Western blot, 0.1-0.5μg/ml, Mouse, Rat
Positive Control
WB: Mouse Brain Tissue,
IHC: rat brain tissue
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of -XRCC3 using anti--XRCC3 antibody (PA1697).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: Mouse Brain Tissue Lysates,
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti--XRCC3 antigen affinity purified polyclonal antibody (Catalog # PA1697) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for -XRCC3 at approximately 38KD. The expected band size for -XRCC3 is at 38KD.
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Figure 2. IHC analysis of XRCC3 using anti-XRCC3 antibody (PA1697).
XRCC3 was detected in paraffin-embedded section of rat brain tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-XRCC3 Antibody (PA1697) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Protein Target Info & Infographic
Gene/Protein Information For Xrcc3 (Source: Uniprot.org, NCBI)
Gene Name
Xrcc3
Full Name
DNA repair protein XRCC3
Weight
38446 MW
Superfamily
RecA family
Alternative Names
DNA repair protein XRCC3;X-ray repair cross-complementing protein 3;Xrcc3; XRCC3 CMM6 X-ray repair cross complementing 3 DNA repair protein XRCC3|X-ray repair complementing defective repair in Chinese hamster cells 3|X-ray repair cross-complementing protein 3
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on Xrcc3, check out the Xrcc3 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for Xrcc3: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-DNA repair protein XRCC3 XRCC3 Antibody Picoband® (PA1697)
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1 Customer Q&As for Anti-DNA repair protein XRCC3 XRCC3 Antibody Picoband®
Question
We are currently using anti-XRCC3 antibody PA1697 for mouse tissue, and we are well pleased with the IHC results. The species of reactivity given in the datasheet says mouse, rat. Is it possible that the antibody can work on pig tissues as well?
Verified Customer
Verified customer
Asked: 2019-05-28
Answer
The anti-XRCC3 antibody (PA1697) has not been validated for cross reactivity specifically with pig tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in pig you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-05-28
