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Product Info Summary
| SKU: | M00240 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IP, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-ATG5/Apg5 Rabbit Monoclonal Antibody
SKU/Catalog Number
M00240
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-ATG5/Apg5 Rabbit Monoclonal Antibody catalog # M00240. Tested in WB, IHC, ICC/IF, IP applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-ATG5/Apg5 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00240)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
GCD-1
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human ATG5
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M00240 is reactive to ATG5 in Human, Mouse, Rat
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
55 kDa
Calculated molecular weight
32447 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00240 is guaranteed for IP, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:1000-1:2000
IHC 1:50-1:200
ICC/IF 1:50-1:200
IP 1:50
Positive Control
WB: human Hela whole cell, human 293T whole cell, human MCF-7 whole cell, human K562 whole cell, rat liver tissue, rat C6 whole cell, mouse liver tissue, mouse NIH/3T3 whole cell
IHC: human ovarian cancer tissue
ICC/IF: Raji cell
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of ATG5/Apg5 using anti-ATG5/Apg5 antibody (M00240).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human 293T whole cell lysates,
Lane 3: human MCF-7 whole cell lysates,
Lane 4: human K562 whole cell lysates,
Lane 5: rat liver tissue lysates,
Lane 6: rat C6 whole cell lysates,
Lane 7: mouse liver tissue lysates,
Lane 8: mouse NIH/3T3 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ATG5/Apg5 antigen affinity purified monoclonal antibody (Catalog # M00240) at 1:1000 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ATG5/Apg5 at approximately 55 kDa. The expected band size for ATG5/Apg5 is at 32 kDa.
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Immunohistochemical analysis of paraffin-embedded human ovarian cancer, using ATG5 Antibody.
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Immunofluorescent analysis of Raji cells, using ATG5 Antibody.
Protein Target Info & Infographic
Gene/Protein Information For ATG5 (Source: Uniprot.org, NCBI)
Gene Name
ATG5
Full Name
Autophagy protein 5
Weight
32447 MW
Superfamily
ATG5 family
Alternative Names
Autophagy protein 5;APG5-like;Apoptosis-specific protein;ATG5;APG5L, ASP; ATG5 APG5, APG5-LIKE, APG5L, ASP, SCAR25, hAPG5 autophagy related 5 autophagy protein 5|APG5 autophagy 5-like|ATG5 autophagy related 5 homolog|apoptosis-specific protein
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on ATG5, check out the ATG5 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for ATG5: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-ATG5/Apg5 Rabbit Monoclonal Antibody (M00240)
Hello CJ!
M00240 has been cited in 3 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Gang Zhong,Huiping Long,Shiting Ma,Yao Shunhan,Jia Li,Jun Yao,miRNA-335-5p relieves chondrocyte inflammation by activating autophagy in osteoarthritis,Life Sciences,Volume 226,2019,Pages 164-172,ISSN 0024-3205,https://doi.org/10.1016/j. lfs.2019.03.071.
Species: Human
M00240 usage in article: APP:WB, SAMPLE:CHONDROCYTES, DILUTION: NA
Nrf2 signalling andautophagyare involved in diabetes mellitus-induced defects in the development of mouse placenta
Anti-autophagic and anti-apoptotic effects of memantine in a SH-SY5Y cell model of Alzheimer's disease via mammalian target of rapamycin-dependent and -independent pathways
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Customer Q&As
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17 Customer Q&As for Anti-ATG5/Apg5 Rabbit Monoclonal Antibody
Question
Our lab used your anti-ATG5/Apg5 Rabbit Monoclonal antibody for IF on embryonic stem cell in the past. I am using human, and We are going to use the antibody for IP next. I am interested in examining embryonic stem cell as well as fetal liver in our next experiment. Could you please give me some suggestion on which antibody would work the best for IP?
Verified Customer
Verified customer
Asked: 2020-04-13
Answer
I have checked the website and datasheets of our anti-ATG5/Apg5 Rabbit Monoclonal antibody and it seems that M00240 has been tested on human in both IF and IP. Thus M00240 should work for your application. Our Boster satisfaction guarantee will cover this product for IP in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for IP detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2020-04-13
Question
Can you help my question with product M00240, anti-ATG5/Apg5 Rabbit Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2020-02-11
Answer
We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M00240 anti-ATG5/Apg5 Rabbit Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2020-02-11
Question
Is this M00240 anti-ATG5/Apg5 Rabbit Monoclonal antibody reactive to the isotypes of ATG5?
Verified Customer
Verified customer
Asked: 2020-02-07
Answer
The immunogen of M00240 anti-ATG5/Apg5 Rabbit Monoclonal antibody is A synthesized peptide derived from human ATG5 . Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2020-02-07
Question
We are currently using anti-ATG5/Apg5 Rabbit Monoclonal antibody M00240 for rat tissue, and we are satisfied with the IF results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on goat tissues as well?
Verified Customer
Verified customer
Asked: 2019-10-23
Answer
The anti-ATG5/Apg5 Rabbit Monoclonal antibody (M00240) has not been validated for cross reactivity specifically with goat tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in goat you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-10-23
Question
We have observed staining in mouse fetal liver. What should we do? Is anti-ATG5/Apg5 Rabbit Monoclonal antibody supposed to stain fetal liver positively?
Verified Customer
Verified customer
Asked: 2019-10-23
Answer
Based on literature fetal liver does express ATG5. Based on Uniprot.org, ATG5 is expressed in colonic mucosa, fetal liver, fetal brain, endometrium placenta, embryonic stem cell, among other tissues. Regarding which tissues have ATG5 expression, here are a few articles citing expression in various tissues:
Embryonic stem cell, Pubmed ID: 11266458
Endometrium, and Placenta, Pubmed ID: 15489334
Fetal brain, Pubmed ID: 17974005
Fetal liver, Pubmed ID: 9563500
Boster Scientific Support
Answered: 2019-10-23
Question
Does M00240 anti-ATG5/Apg5 Rabbit Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-09-17
Answer
It shows on the product datasheet, M00240 anti-ATG5/Apg5 Rabbit Monoclonal antibody as been validated on IF. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-09-17
Question
See attached the WB image, lot number and protocol we used for endometrium placenta using anti-ATG5/Apg5 Rabbit Monoclonal antibody M00240. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-06-07
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-06-07
Question
Is a blocking peptide available for product anti-ATG5/Apg5 Rabbit Monoclonal antibody (M00240)?
E. Rodriguez
Verified customer
Asked: 2018-12-28
Answer
We do provide the blocking peptide for product anti-ATG5/Apg5 Rabbit Monoclonal antibody (M00240). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2018-12-28
Question
Our team were well pleased with the WB result of your anti-ATG5/Apg5 Rabbit Monoclonal antibody. However we have seen positive staining in colonic mucosa cytoplasm. using this antibody. Is that expected? Could you tell me where is ATG5 supposed to be expressed?
A. Parker
Verified customer
Asked: 2018-11-29
Answer
According to literature, colonic mucosa does express ATG5. Generally ATG5 expresses in cytoplasm. Regarding which tissues have ATG5 expression, here are a few articles citing expression in various tissues:
Embryonic stem cell, Pubmed ID: 11266458
Endometrium, and Placenta, Pubmed ID: 15489334
Fetal brain, Pubmed ID: 17974005
Fetal liver, Pubmed ID: 9563500
Boster Scientific Support
Answered: 2018-11-29
Question
We want using your anti-ATG5/Apg5 Rabbit Monoclonal antibody for autophagy of mitochondrion studies. Has this antibody been tested with western blotting on raji cell lysate? We would like to see some validation images before ordering.
P. Krishna
Verified customer
Asked: 2018-07-12
Answer
We appreciate your inquiry. This M00240 anti-ATG5/Apg5 Rabbit Monoclonal antibody is tested on raji cell lysate. It is guaranteed to work for IP, IF, WB in human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2018-07-12
Question
I was wanting to use your anti-ATG5/Apg5 Rabbit Monoclonal antibody for IF for human endometrium placenta on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human endometrium placenta identification?
Verified Customer
Verified customer
Asked: 2018-06-22
Answer
It shows on the product datasheet, M00240 anti-ATG5/Apg5 Rabbit Monoclonal antibody has been tested for IP, IF, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in human endometrium placenta in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2018-06-22
Question
I see that the anti-ATG5/Apg5 Rabbit Monoclonal antibody M00240 works with IF, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2018-01-04
Answer
You can find protocols for IF on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2018-01-04
Question
Does anti-ATG5/Apg5 Rabbit Monoclonal antibody M00240 work for IF with endometrium placenta?
Verified Customer
Verified customer
Asked: 2017-07-20
Answer
According to the expression profile of endometrium placenta, ATG5 is highly expressed in endometrium placenta. So, it is likely that anti-ATG5/Apg5 Rabbit Monoclonal antibody M00240 will work for IF with endometrium placenta.
Boster Scientific Support
Answered: 2017-07-20
Question
Thank you for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for endometrium placenta using anti-ATG5/Apg5 Rabbit Monoclonal antibody M00240. Let me know if you need anything else.
E. Mangal
Verified customer
Asked: 2016-10-04
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2016-10-04
Question
Does anti-ATG5/Apg5 Rabbit Monoclonal antibody M00240 work on zebrafish IF with endometrium placenta?
S. Singh
Verified customer
Asked: 2015-08-14
Answer
Our lab technicians have not validated anti-ATG5/Apg5 Rabbit Monoclonal antibody M00240 on zebrafish. You can run a BLAST between zebrafish and the immunogen sequence of anti-ATG5/Apg5 Rabbit Monoclonal antibody M00240 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated zebrafish samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in zebrafish endometrium placenta in IF, you can get your next antibody for free.
Boster Scientific Support
Answered: 2015-08-14
Question
Is there a BSA free version of anti-ATG5/Apg5 Rabbit Monoclonal antibody M00240 available?
T. Li
Verified customer
Asked: 2013-10-21
Answer
Thank you for your recent telephone inquiry. I can confirm that some lots of this anti-ATG5/Apg5 Rabbit Monoclonal antibody M00240 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2013-10-21
Question
you antibody to test anti-ATG5/Apg5 Rabbit Monoclonal antibody M00240 on human endometrium placenta for research purposes, then I may be interested in using anti-ATG5/Apg5 Rabbit Monoclonal antibody M00240 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
A. Krishna
Verified customer
Asked: 2013-04-05
Answer
The products we sell, including anti-ATG5/Apg5 Rabbit Monoclonal antibody M00240, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2013-04-05
