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- Table of Contents
3 Citations 17 Q&As
1 Citations
Facts about Autophagy protein 5.
Involved in mitochondrial quality control after oxidative damage, and in following cellular longevity. Plays a critical role in a number of facets of lymphocyte development and is critical for both B and T lymphocyte survival and proliferation.
Human | |
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Gene Name: | ATG5 |
Uniprot: | Q9H1Y0 |
Entrez: | 9474 |
Belongs to: |
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ATG5 family |
APG5 autophagy 5-like (S. cerevisiae); APG5; APG5LS. cerevisiae)-like; ASP; ASPAPG5-LIKE; ATG5 autophagy related 5 homolog (S. cerevisiae); ATG5; Autophagy protein 5
Mass (kDA):
32.447 kDA
Human | |
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Location: | 6q21 |
Sequence: | 6; NC_000006.12 (106184476..106325820, complement) |
Ubiquitous. The mRNA is present at similar levels in viable and apoptotic cells, whereas the protein is dramatically highly expressed in apoptotic cells.
Cytoplasm. Preautophagosomal structure membrane; Peripheral membrane protein. Colocalizes with nonmuscle actin. The conjugate detaches from the membrane immediately before or after autophagosome formation is completed (By similarity). Localizes also to discrete punctae along the ciliary axoneme and to the base of the ciliary axoneme.
Many studies have found that ATG5 can be used as a protein marker. It is a member from the cytokinesis genes family and has been associated with the development Alzheimer's Disease and Parkinson's Disease. A patient must have ATG5 present to be able to detect it. A sample with ATG5 deficiency is unlikely to show any response in many cases.
The fascinating association between sepsis is and the ATG5 gene marker is remarkable. This protein interacts directly with other genes such as interleukin converting enzyme and nucleotide oligomerization protein 1. ATG5 polymorphisms cause increased monocyte inflammatory and cytokine expression. Boster's antibodies along with the ATG5 marker play important parts in the immune system. However the ATG5 variant appears to play a greater role.
Recent research has shown ATG5 polymorphisms have an impact on patients' predispositions to inflammatory disorders. The polymorphisms alter ATG5 expression levels and autophagy activity. In addition, the ATG5 promoter polymorphisms are associated with Paget bone disease, psoriasis, and Crohn's disease. The ATG5 promoter polymorphisms also correlate with sepsis severity and progression.
The cell lysates were electrophoresed using polyacrylamide gels. The proteins were transferred to PVDF membranes. They were incubated overnight at 4 degrees Celsius. Secondary antibodies were added to detect ATG5 protein. The PVDF membranes were then visualized with enhanced chemiluminescence detection kit. These methods are based on the principle that the autophagy protein ATG5 regulates the secretion of TNF-a and IL-1b.
The relationship between ATG5 & the ATG5 genes has many aspects that are important for research. The ATG5 gene is required for adipogenesis, and is involved in B-cell receptor polarization, antigen presentation, and apoptotic cell death. Atg5 interacts to Atg12, a ubiquitin like conjugation system, which mediates host protection.
The SYBRII Green PCR master mix, 10181-2-AP, targets ATG5 and is suitable for IHC and IF. It can also used for FC and ELISA. The primers used in the experiment were CGGTCGGGCGCTTCTGTAGTGT. The final volume of the PCR mixture is 10 mL. ATG5 amplification was then conducted.
These Boster Bio ATG5 ELISA (enzyme-linked immunosorbent assay) kits are intended for the quantitative measurement of ATG5 in rat tissue homogenates, cell lysates, and other biological fluids. They contain all the necessary reagents for a one-step assay. The kit can also come in bulk. To ensure reproducible results in the laboratory, the conditions should be monitored and the assay should all be performed by one operator.
This ELISA kit detects ATG5 within a variety of biological samples. It is particularly useful in research on cellular toxicity. It can be used in a variety of biological samples to determine the relative levels ATF5 within cells and tissue cultures, as well as to screen for effects of various activators and treatments on ATG5 transcription. Although the kit is not sold separately, Boster Rewards can be earned by purchasing multiple ELISA kits.
The ATG5 ELISA kits have a sensitivity that can be increased up to picograms. High-specificity antibodies manufactured by the company have been tested against a variety samples. Images and validation protocols are available upon request. The Picoband polymer-based secondary antibody saves 30 minutes in immunohistochemistry (IHC). Sanbio, the distributor of BeNeLux provides technical support for the technology.
The Boster Bio ATG5 ELISA kit MBS2709569 is a microwell, strip plate ELISA kit designed for detection of Autophagy related protein 5 (ATG5) in biological samples. This kit uses an antibody derived from rabbit and mouse to detect native ATG5 in biological samples. The kit is able to detect native ATG5 in biological samples. It also has high intra and cross-assay CVs. This kit's high reproducibility makes it an excellent choice for research and diagnostic applications.
The ATG5 glycoprotein is a novel glycoprotein that plays a role in cell death signaling. Boster Bio ATG5 biological assays allow researchers to measure the level of ATG5 protein in different cell types. The Boster Bio ATG5 antibody reacts with a broad range of human, mouse, and rat cells. Using a proprietary ELISA platform, this kit delivers highly sensitive ELISA kits.
Transfected BIRC7 KD cells with siRNA specific to ATG5/BECN1 were used. In three independent experiments, the number of RFP-only puntas was determined. Data are reported in means + S.E.M. for each sample. The BAC buffer was prepared using standard RPMI and 10% FBS. The cells were cultured for 14 days in RPMI.
ATG5 is an important component of autophagy. It is a key regulator in autophagy and is involved with the progression of many types cancers. ATG5 inhibition results in increased triglyceride level and larger lipid droplet sizes. ATG5-12 is also recruited by E3 Ubiquitin Ligase. ATG5 expression promotes pancreatic carcinoma progression and increases autophagy.
The ATG5 knockdown reduces autophagy and causes EMT-like mutations in PTC cells. The inhibitors of ATG5 do not affect BIRC7 expression. They do however increase PTC cell migration after 3-MA treatment. The results are shown as mean + SE.M. (SD = standard error).
Autophagy is a key therapeutic target in cancer treatment and neurodegenerative diseases. But, this process can have a double-edged blade effect. Autophagy is also implicated in skin tumorigenesis. The gene ATG7 is responsible for UV radiation-induced inflammation. Patients may be less likely to survive if the drug inhibits autophagy. Although there is no conclusive evidence of autophagy's role in cancer, the results are encouraging.
Boster Biological Technologies customer service is available to assist you with any problems with your order. It is crucial to inspect your order carefully, particularly if you have ordered the wrong product. If you are not satisfied with the product, please notify the company. In some cases, they will exchange the product for free. In others, you will need to pay shipping charges. Boster Bio cannot accept returns if a product was customized to your needs.
Boster Bio has a dedicated staff to assist customers. The team can be reached 24 hours a-day to answer any questions. They can answer technical questions or offer tips and tricks for your project. They can also arrange custom services, such as BeNeLux deliveries, if needed. They can help you order the product that you need and provide many other resources such as a complete reference manual.
In the event your purchase becomes defective, Boster Biological Technology will provide you with a replacement or refund. You must notify Boster Biological Technology within 12 months of receiving your purchase to be eligible for a refund. A customer service representative will review the order and protocol to determine if the problem is related directly to the product. If not, Boster Bio will provide reasonable independent advice and replace your product. The team will get back to you as soon as possible in order to resolve the problem.
Immunohistochemistry uses specialized techniques to detect antigens in cells. To provide the highest quality ELISA kits and antibodies, Boster Bio's expert team will optimize the antibodies, dilution ratio, and sample types for optimal results. Multiplex Immunohistochemistry service is offered by Boster Bio. This allows for multiple targets to be detected in one sample, which increases screening throughput.
PMID: 9563500 by Hammond E.M., et al. Homology between a human apoptosis specific protein and the product of APG5, a gene involved in autophagy in yeast.
PMID: 7796880 by Grand R.J.A., et al. A novel protein expressed in mammalian cells undergoing apoptosis.
*More publications can be found for each product on its corresponding product page