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Product Info Summary
| SKU: | M04951 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-ATF7 Rabbit Monoclonal Antibody
SKU/Catalog Number
M04951
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-ATF7 Rabbit Monoclonal Antibody catalog # M04951. Tested in WB application. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-ATF7 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M04951)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
HBD-1
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human ATF7
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M04951 is reactive to ATF7 in Human, Mouse, Rat
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
45 kDa
Calculated molecular weight
52967 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M04951 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:1000
Validation Images & Assay Conditions
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All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.
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All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.
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Western blot analysis of ATF7 expression in (1) Raji cell lysate; (2) Raw 264.7 cell lysate.
Protein Target Info & Infographic
Gene/Protein Information For ATF7 (Source: Uniprot.org, NCBI)
Gene Name
ATF7
Full Name
Cyclic AMP-dependent transcription factor ATF-7
Weight
52967 MW
Superfamily
bZIP family
Alternative Names
Cyclic AMP-dependent transcription factor ATF-7;cAMP-dependent transcription factor ATF-7;Activating transcription factor 7;Transcription factor ATF-A;ATF7;ATFA; ATF7 ATFA activating transcription factor 7 cyclic AMP-dependent transcription factor ATF-7|transcription factor ATF-A
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on ATF7, check out the ATF7 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for ATF7: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-ATF7 Rabbit Monoclonal Antibody (M04951)
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Customer Q&As
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6 Customer Q&As for Anti-ATF7 Rabbit Monoclonal Antibody
Question
I have attached the WB image, lot number and protocol we used for popliteal artery using anti-ATF7 Rabbit Monoclonal antibody M04951. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-07-03
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-07-03
Question
I was wanting to use to test anti-ATF7 Rabbit Monoclonal antibody M04951 on rat popliteal artery for research purposes, then I may be interested in using anti-ATF7 Rabbit Monoclonal antibody M04951 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-07-02
Answer
The products we sell, including anti-ATF7 Rabbit Monoclonal antibody M04951, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-07-02
Question
We are currently using anti-ATF7 Rabbit Monoclonal antibody M04951 for human tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on zebrafish tissues as well?
Verified Customer
Verified customer
Asked: 2018-08-03
Answer
The anti-ATF7 Rabbit Monoclonal antibody (M04951) has not been tested for cross reactivity specifically with zebrafish tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in zebrafish you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2018-08-03
Question
Thanks for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for popliteal artery using anti-ATF7 Rabbit Monoclonal antibody M04951. Let me know if you need anything else.
E. Li
Verified customer
Asked: 2018-05-18
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-05-18
Question
My question regarding product M04951, anti-ATF7 Rabbit Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
M. Thomas
Verified customer
Asked: 2017-06-12
Answer
We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M04951 anti-ATF7 Rabbit Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2017-06-12
Question
Is there a BSA free version of anti-ATF7 Rabbit Monoclonal antibody M04951 available?
S. Carter
Verified customer
Asked: 2017-03-31
Answer
I appreciate your recent telephone inquiry. I can confirm that some lots of this anti-ATF7 Rabbit Monoclonal antibody M04951 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2017-03-31
