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Product Info Summary
| SKU: | M00979-2 |
|---|---|
| Size: | 100uL |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | IHC, WB |
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Product info
Product Name
Anti-S100-beta Rabbit Monoclonal Antibody, Clone#RM304
SKU/Catalog Number
M00979-2
Size
100uL
Form
Liquid
Description
Boster Bio Anti-S100-beta Rabbit Monoclonal Antibody, Clone#RM304 (Catalog # M00979-2). Tested in IHC, WB applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-S100-beta Rabbit Monoclonal Antibody, Clone#RM304 (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00979-2)
Host
Rabbit
Contents
50% Glycerol/PBS with 1% BSA and 0.09% sodium azide
Clonality
Monoclonal
Clone Number
RM304
Isotype
Rabbit IgG
Immunogen
A peptide corresponding to the C-terminus of human S100-B
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
This antibody reacts to human S100-B (S-100 protein beta chain). It may also react to mouse or rat S100-B, as predicted by immunogen homology.
Reactive Species
M00979-2 is reactive to S100B in Human
Applications
M00979-2 is guaranteed for IHC, WB Boster Guarantee
Observed Molecular Weight
Calculated molecular weight
10713 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry (IHC): 1:500-1:1000 dilution
WB: 1:100-1:200 dilution.
Validation Images & Assay Conditions
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Figure 1. Western Blotting result
Western Blot of human brain tissue lysate using anti-S100B rabbit monoclonal antibody (Clone RM304) at a 1:100 dilution.
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Figure 2. IHC result
Immunohistochemical staining of formalin fixed and paraffin embedded human brain tissue section using anti-S100B rabbit monoclonal antibody (Clone RM304) at a 1:1000 dilution.
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Figure 3. IHC result
Immunohistochemical staining of formalin fixed and paraffin embedded human breast cancer tissue section using anti-S100B rabbit monoclonal antibody (Clone RM304) at a 1:1000 dilution.
Protein Target Info & Infographic
Gene/Protein Information For S100B (Source: Uniprot.org, NCBI)
Gene Name
S100B
Full Name
Protein S100-B
Weight
10713 MW
Superfamily
S-100 family
Alternative Names
beta (neural); NEF; S100 beta; S100 calcium binding protein B; S100 calcium-binding protein B; S100 calcium-binding protein, beta (neural); S-100 calcium-binding protein, beta chain, 10protein S100-B; S-100 protein beta chain; S-100 protein subunit beta; S100; S100B; S100beta S100B NEF, S100, S100-B, S100beta S100 calcium binding protein B protein S100-B|S-100 calcium-binding protein, beta chain|S-100 protein subunit beta|S100 calcium-binding protein, beta (neural)
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on S100B, check out the S100B Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for S100B: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-S100-beta Rabbit Monoclonal Antibody, Clone#RM304 (M00979-2)
Hello CJ!
M00979-2 has been cited in 5 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Expression of S100B during the innate immune of corneal epithelium against fungi invasion
Up-regulation of P2X7 receptors mediating proliferation of Schwann cells after sciatic nerve injury
Zhao F, He W, Zhang Y, Tian D, Zhao H, Yu K, Bai J. Neural Regen Res. 2013 Jul 25;8(21):1974-84. Doi: 10.3969/J.Issn.1673-5374.2013.21.006. Electric Stimulation And Decimeter Wave Therapy Improve The Recovery Of Injured Sciatic Nerves.
Wu Y, Xue B, Li X, Liu H. Neural Regen Res. 2012 Nov 25;7(33):2583-91. Doi: 10.3969/J.Issn.1673-5374.2012.33.003. Puerarin Prevents High Glucose-Induced Apoptosis Of Schwann Cells By Inhibiting Oxidative Stress.
Xue B, Sun L, Li X, Wang X, Zhang Y, Mu Y, Liang L. Neural Regen Res. 2012 Oct 25;7(30):2340-6. Doi: 10.3969/J.Issn.1673-5374.2012.30.003. Ginsenoside Rb1 Relieves Glucose Fluctuation-Induced Oxidative Stress And Apoptosis In Schwann Cells.
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Customer Reviews
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Customer Q&As
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16 Customer Q&As for Anti-S100-beta Rabbit Monoclonal Antibody, Clone#RM304
Question
Is this M00979-2 anti-S100-beta Rabbit Monoclonal antibody, Clone#RM304 reactive to the isotypes of S100B?
Verified Customer
Verified customer
Asked: 2020-02-26
Answer
The immunogen of M00979-2 anti-S100-beta Rabbit Monoclonal antibody, Clone#RM304 is A peptide corresponding to the C-terminus of human S100-B. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2020-02-26
Question
Our lab used your anti-S100-beta Rabbit Monoclonal antibody, Clone
Verified Customer
Verified customer
Asked: 2020-02-25
Answer
I looked at the website and datasheets of our anti-S100-beta Rabbit Monoclonal antibody, Clone
Boster Scientific Support
Answered: 2020-02-25
Question
Can you help my question with product M00979-2, anti-S100-beta Rabbit Monoclonal antibody, Clone#RM304. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
S. Miller
Verified customer
Asked: 2020-02-04
Answer
It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M00979-2 anti-S100-beta Rabbit Monoclonal antibody, Clone#RM304, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2020-02-04
Question
We are interested in using your anti-S100-beta Rabbit Monoclonal antibody, Clone#RM304 for axonogenesis studies. Has this antibody been tested with western blotting on breast cancer tissue? We would like to see some validation images before ordering.
Verified Customer
Verified customer
Asked: 2020-01-31
Answer
Thank you for your inquiry. This M00979-2 anti-S100-beta Rabbit Monoclonal antibody, Clone#RM304 is tested on human brain tissue, tissue lysate, breast cancer tissue. It is guaranteed to work for IHC, WB in human. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2020-01-31
Question
My team were well pleased with the WB result of your anti-S100-beta Rabbit Monoclonal antibody, Clone
Verified Customer
Verified customer
Asked: 2019-12-31
Answer
Based on literature, c1 segment of cervical spinal cord does express S100B. Generally S100B expresses in cytoplasm. Regarding which tissues have S100B expression, here are a few articles citing expression in various tissues:
Skin, Pubmed ID: 15489334
Boster Scientific Support
Answered: 2019-12-31
Question
Is there a BSA free version of anti-S100-beta Rabbit Monoclonal antibody, Clone#RM304 M00979-2 available?
Verified Customer
Verified customer
Asked: 2019-12-18
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-S100-beta Rabbit Monoclonal antibody, Clone#RM304 M00979-2 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-12-18
Question
I was wanting to use your anti-S100-beta Rabbit Monoclonal antibody, Clone#RM304 for IHC for human skin on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human skin identification?
Verified Customer
Verified customer
Asked: 2019-11-15
Answer
You can see on the product datasheet, M00979-2 anti-S100-beta Rabbit Monoclonal antibody, Clone#RM304 has been validated for IHC, WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human skin in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-11-15
Question
Does M00979-2 anti-S100-beta Rabbit Monoclonal antibody, Clone#RM304 work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-09-23
Answer
You can see on the product datasheet, M00979-2 anti-S100-beta Rabbit Monoclonal antibody, Clone#RM304 as been tested on IHC. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-09-23
Question
I would like to test anti-S100-beta Rabbit Monoclonal antibody, Clone#RM304 M00979-2 on human skin for research purposes, then I may be interested in using anti-S100-beta Rabbit Monoclonal antibody, Clone#RM304 M00979-2 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-08-05
Answer
The products we sell, including anti-S100-beta Rabbit Monoclonal antibody, Clone#RM304 M00979-2, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-08-05
Question
I see that the anti-S100-beta Rabbit Monoclonal antibody, Clone#RM304 M00979-2 works with IHC, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-07-22
Answer
You can find protocols for IHC on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2019-07-22
Question
We are currently using anti-S100-beta Rabbit Monoclonal antibody, Clone#RM304 M00979-2 for human tissue, and we are content with the IHC results. The species of reactivity given in the datasheet says human. Is it likely that the antibody can work on zebrafish tissues as well?
Verified Customer
Verified customer
Asked: 2019-05-06
Answer
The anti-S100-beta Rabbit Monoclonal antibody, Clone#RM304 (M00979-2) has not been tested for cross reactivity specifically with zebrafish tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in zebrafish you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-05-06
Question
I have attached the WB image, lot number and protocol we used for skin using anti-S100-beta Rabbit Monoclonal antibody, Clone#RM304 M00979-2. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-01-29
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-01-29
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for skin using anti-S100-beta Rabbit Monoclonal antibody, Clone#RM304 M00979-2. Let me know if you need anything else.
J. Baker
Verified customer
Asked: 2016-12-20
Answer
I appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2016-12-20
Question
Will anti-S100-beta Rabbit Monoclonal antibody, Clone#RM304 M00979-2 work for IHC with skin?
E. Brown
Verified customer
Asked: 2016-08-03
Answer
According to the expression profile of skin, S100B is highly expressed in skin. So, it is likely that anti-S100-beta Rabbit Monoclonal antibody, Clone#RM304 M00979-2 will work for IHC with skin.
Boster Scientific Support
Answered: 2016-08-03
Question
Is a blocking peptide available for product anti-S100-beta Rabbit Monoclonal antibody, Clone#RM304 (M00979-2)?
B. Parker
Verified customer
Asked: 2014-07-22
Answer
We do provide the blocking peptide for product anti-S100-beta Rabbit Monoclonal antibody, Clone#RM304 (M00979-2). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2014-07-22
Question
We have observed staining in human c1 segment of cervical spinal cord. What should we do? Is anti-S100-beta Rabbit Monoclonal antibody, Clone#RM304 supposed to stain c1 segment of cervical spinal cord positively?
M. Anderson
Verified customer
Asked: 2013-08-30
Answer
According to literature c1 segment of cervical spinal cord does express S100B. According to Uniprot.org, S100B is expressed in c1 segment of cervical spinal cord, skin, among other tissues. Regarding which tissues have S100B expression, here are a few articles citing expression in various tissues:
Skin, Pubmed ID: 15489334
Boster Scientific Support
Answered: 2013-08-30
