Product Info Summary
SKU: | M04085 |
---|---|
Size: | 100 μl |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | Flow Cytometry, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-Aspartate Aminotransferase Monoclonal Antibody
SKU/Catalog Number
M04085
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-Aspartate Aminotransferase Monoclonal Antibody catalog # M04085. Tested in WB, IHC, ICC/IF, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Aspartate Aminotransferase Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M04085)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
ADEA-7
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human Aspartate Aminotransferase Plays a key role in amino acid metabolism.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M04085 is reactive to GOT1 in Human, Mouse, Rat
Applications
M04085 is guaranteed for Flow Cytometry, IF, IHC, ICC, WB Boster Guarantee
Observed Molecular Weight
190 kDa
Calculated molecular weight
112035 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Assay dilution & Images
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:50-1:200
ICC/IF 1:50-1:200
FC 1:50
Validation Images & Assay Conditions
![m04085 wb m04085 wb](https://www.bosterbio.com/media/catalog/product/m/0/m04085-wb.jpg)
Click image to see more details
Western blot analysis of Aspartate Aminotransferase expression in MCF7 cell lysate.
Protein Target Info & Infographic
Gene/Protein Information For GOT1 (Source: Uniprot.org, NCBI)
Gene Name
GOT1
Full Name
Aspartate aminotransferase, cytoplasmic
Weight
112035 MW
Superfamily
class-I pyridoxal-phosphate-dependent aminotransferase family
Alternative Names
aspartate aminotransferase, cytoplasmic; EC 2.6.1.1; GIG18; Glutamate oxaloacetate transaminase 1; glutamic-oxaloacetic transaminase 1, soluble (aspartate aminotransferase 1); growth-inhibiting protein 18; Transaminase A GOT1 AST1, ASTQTL1, GIG18, cAspAT, cCAT glutamic-oxaloacetic transaminase 1 aspartate aminotransferase, cytoplasmic|aspartate aminotransferase 1|aspartate transaminase 1|cysteine aminotransferase, cytoplasmic|cysteine transaminase, cytoplasmic|glutamate oxaloacetate transaminase 1|glutamic-oxaloacetic transaminase 1, soluble|growth-inhibiting protein 18|testis secretory sperm-binding protein Li 196a|transaminase A
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on GOT1, check out the GOT1 Infographic
![GOT1 infographic](/media/images/gene-infographic-example.jpg)
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for GOT1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Aspartate Aminotransferase Monoclonal Antibody (M04085)
Hello CJ!
M04085 has been cited in 1 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Liver Injury and Tumor-Inhibiting Effect of Sequential Transcatheter Arterial Chemoembolization and Portal Venous Embolization on Rabbit VX2 Liver %u2026
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Customer Reviews
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Customer Q&As
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5 Customer Q&As for Anti-Aspartate Aminotransferase Monoclonal Antibody
Question
I am interested in to test anti-Aspartate Aminotransferase Monoclonal antibody M04085 on mouse brain for research purposes, then I may be interested in using anti-Aspartate Aminotransferase Monoclonal antibody M04085 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
S. Carter
Verified customer
Asked: 2019-09-10
Answer
The products we sell, including anti-Aspartate Aminotransferase Monoclonal antibody M04085, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-09-10
Question
I was wanting to use your anti-Aspartate Aminotransferase Monoclonal antibody for IF for mouse brain on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for mouse brain identification?
Verified Customer
Verified customer
Asked: 2018-01-25
Answer
As indicated on the product datasheet, M04085 anti-Aspartate Aminotransferase Monoclonal antibody has been validated for IF, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in mouse brain in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2018-01-25
Question
Does M04085 anti-Aspartate Aminotransferase Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2017-12-01
Answer
As indicated on the product datasheet, M04085 anti-Aspartate Aminotransferase Monoclonal antibody as been tested on IF. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2017-12-01
Question
Does anti-Aspartate Aminotransferase Monoclonal antibody M04085 work for IF with brain?
C. Gonzalez
Verified customer
Asked: 2013-10-16
Answer
According to the expression profile of brain, GOT1 is highly expressed in brain. So, it is likely that anti-Aspartate Aminotransferase Monoclonal antibody M04085 will work for IF with brain.
Boster Scientific Support
Answered: 2013-10-16
Question
We are currently using anti-Aspartate Aminotransferase Monoclonal antibody M04085 for rat tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on zebrafish tissues as well?
S. Krishna
Verified customer
Asked: 2013-03-05
Answer
The anti-Aspartate Aminotransferase Monoclonal antibody (M04085) has not been validated for cross reactivity specifically with zebrafish tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in zebrafish you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2013-03-05