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Product Info Summary
| SKU: | A03284 |
|---|---|
| Size: | 100ul |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | ELISA, IHC-P, WB, IF (paraffin) |
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Product info
Product Name
Anti-GATA-5 Antibody
SKU/Catalog Number
A03284
Size
100ul
Form
Liquid
Description
Boster Bio Anti-GATA-5 Antibody (Catalog# A03284). Tested in WB, IHC-P, IF (paraffin), ELISA applications. This antibody reacts with Human, Mouse.
Storage & Handling
Store at -20°C for one year. For short-term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-GATA-5 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A03284)
Host
Rabbit
Contents
PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A peptide derived from human GATA-5. Immunogen sequence location: 244 - 293
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
A03284 is reactive to GATA5 in Human, Mouse
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
72 kDa
Calculated molecular weight
41299 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A03284 is guaranteed for ELISA, IHC-P, WB, IF (paraffin) Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western Blot, 1:500 - 1:2000
Immunohistochemistry, 1:100 - 1:300
ELISA, 1:10000
Not yet tested in other applications.
Validation Images & Assay Conditions
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Western blot analysis of lysate from NIH/3T3 cells, using GATA-5 antibody.
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Immunohistochemistry analysis of GATA-5 antibody in paraffin-embedded human lung carcinoma tissue.
Protein Target Info & Infographic
Gene/Protein Information For GATA5 (Source: Uniprot.org, NCBI)
Gene Name
GATA5
Full Name
Transcription factor GATA-5
Weight
41299 MW
Alternative Names
GATA5;Transcription factor GATA-5;GATA-binding factor 5 Gata5|GATA binding protein 5|transcription factor GATA-5|GATA-binding factor 5|Transcription factor GATA-5 (GATA binding factor-5)
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on GATA5, check out the GATA5 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for GATA5: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-GATA-5 Antibody (A03284)
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No publications found for A03284
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Customer Q&As
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4 Customer Q&As for Anti-GATA-5 Antibody
Question
Is this A03284 anti-GATA-5 antibody reactive to the isotypes of GATA5?
Verified Customer
Verified customer
Asked: 2020-03-25
Answer
The immunogen of A03284 anti-GATA-5 antibody is A peptide derived from human GATA-5. Immunogen sequence location: 244 - 293. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2020-03-25
Question
Would A03284 anti-GATA-5 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-09-25
Answer
As indicated on the product datasheet, A03284 anti-GATA-5 antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-09-25
Question
Can you help my question with product A03284, anti-GATA-5 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-07-16
Answer
It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A03284 anti-GATA-5 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-07-16
Question
My question regards to test anti-GATA-5 antibody A03284 on mouse uterus for research purposes, then I may be interested in using anti-GATA-5 antibody A03284 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
N. Banerjee
Verified customer
Asked: 2015-02-19
Answer
The products we sell, including anti-GATA-5 antibody A03284, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2015-02-19
