Click image to see more details
Product Info Summary
| SKU: | PB9200 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | WB |
Customers Who Bought This Also Bought
Product info
Product Name
Anti-GATA5 Antibody Picoband®
SKU/Catalog Number
PB9200
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-GATA5 Antibody Picoband® catalog # PB9200. Tested in WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-GATA5 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9200)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human GATA5 recombinant protein (Position: M1-A397). Human GATA5 shares 75% amino acid (aa) sequence identity with mouse GATA5.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PB9200 is reactive to GATA5 in Human
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
50 kDa
Calculated molecular weight
41299 MW
Background of GATA-5
Transcription factor GATA-5, also named GATAS or GATA5, is a protein that in humans is encoded by the GATA5 gene. It is mapped to 20q13.33. The protein encoded by this gene is a transcription factor that contains two GATA-type zinc fingers. The encoded protein is known to bind to hepatocyte nuclear factor-1alpha (HNF-1alpha), and this interaction is essential for cooperative activation of the intestinal lactase-phlorizin hydrolase promoter. In other organisms, similar proteins may be involved in the establishment of cardiac smooth muscle cell diversity. In addition, the expression of GATA5 increased in a human colon cancer cell line within 2 hours of exposure to sodium butyrate, which induces differentiation.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PB9200 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human
Positive Control
WB: human 293T whole cell, human HepG2 whole cell
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of GATA5 using anti-GATA5 antibody (PB9200).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human 293T whole cell lysates,
Lane 2: human HepG2 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GATA5 antigen affinity purified polyclonal antibody (Catalog # PB9200) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GATA5 at approximately 50 kDa. The expected band size for GATA5 is at 41 kDa.
Protein Target Info & Infographic
Gene/Protein Information For GATA5 (Source: Uniprot.org, NCBI)
Gene Name
GATA5
Full Name
Transcription factor GATA-5
Weight
41299 MW
Alternative Names
Transcription factor GATA-5;GATA-binding factor 5;GATA5 ; Gata5|GATA binding protein 5|transcription factor GATA-5|GATA-binding factor 5|Transcription factor GATA-5 (GATA binding factor-5)
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on GATA5, check out the GATA5 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for GATA5: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-GATA5 Antibody Picoband® (PB9200)
Hello CJ!
No publications found for PB9200
*Do you have publications using this product? Share with us and receive a reward. Ask us for more details.
Recommended Resources
Here are featured tools and databases that you might find useful.
- Boster's Pathways Library
- Protein Databases
- Bioscience Research Protocol Resources
- Data Processing & Analysis Software
- Photo Editing Software
- Scientific Literature Resources
- Research Paper Management Tools
- Molecular Biology Software
- Primer Design Tools
- Bioinformatics Tools
- Phylogenetic Tree Analysis
Customer Reviews
Have you used Anti-GATA5 Antibody Picoband®?
Submit a review and receive an Amazon gift card.
- $30 for a review with an image
0 Reviews For Anti-GATA5 Antibody Picoband®
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question
1 Customer Q&As for Anti-GATA5 Antibody Picoband®
Question
We are currently using anti-GATA5 antibody PB9200 for human tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human, rat. Is it true that the antibody can work on feline tissues as well?
Verified Customer
Verified customer
Asked: 2018-08-13
Answer
The anti-GATA5 antibody (PB9200) has not been validated for cross reactivity specifically with feline tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in feline you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2018-08-13
