ASIC3 Antibodies

Acid-sensing ion channel 3 (ASIC3)

Cation channel with high affinity for sodium, which is gated by extracellular protons and inhibited by the diuretic amiloride. Generates a biphasic current with a fast inactivating and a slow sustained phase.

In sensory neurons is suggested to mediate the pain induced by acidosis occurring in ischemic, damaged or inflamed tissue. May be involved in hyperalgesia.

Gene Information

Human
Gene Name:	ASIC3
Uniprot:	Q9UHC3
Entrez:	9311

Family

Belongs to:
amiloride-sensitive sodium channel (TC 1.A.6) family

Alternative Names

Acid-sensing ion channel 3; amiloride-sensitive cation channel 3; amiloride-sensitive cation channel 3, testis; ASIC3hTNaC1; DRASIC; hASIC3; modulatory subunit of ASIC2a; Neuronal amiloride-sensitive cation channel 3; proton-gated cation channel subunit; SLNAC1; Testis sodium channel 1; TNaC1

Molecular Weight

Mass (kDA):
58.905 kDA

Genomic Context

Human
Location:	7q36.1
Sequence:	7; NC_000007.14 (151048292..151052756)

Tissue Specificity

Expressed by sensory neurons. Strongly expressed in brain, spinal chord, lung, lymph nodes, kidney, pituitary, heart and testis.

Subcellular Localizations

Cell membrane; Multi-pass membrane protein. Cytoplasm. Cell surface expression may be stabilized by interaction with LIN7B and cytoplasmic retention by interaction with DLG4. In part cytoplasmic in cochlea cells (By similarity).

Diseases

• Pain
• Acidosis
• Inflammation
• Nervousness
• Hyperalgesia
• Ischemia
• Hyperalgesias, Mechanical
• Myocardial Ischemia
• Chronic Pain
• Arthritis
• Myalgia
• Angina Pectoris

• Myositis
• Neoplasms
• Visceral Pain
• Myocardial Infarction
• Nerve Degeneration
• Thermal Hyperalgesia

Pathways

• Localization
• Sensitization
• Hypersensitivity
• Reflex
• Synaptic Transmission
• Membrane Depolarization
• Pathogenesis
• Transport
• Sensory Perception
• Response To Acid
• Secretion
• Osteoclast Differentiation
• Cell Migration

• Muscle Cell Migration
• Brain Development
• Aging
• Muscle Contraction
• Taste Perception
• Transepithelial Transport
• Chemotaxis

PTMs

• Phosphorylation

• Methylation

• Palmitoylation

Research Areas

• Neuroscience

For details, visit:
bosterbio.com/bosterbio-gene-info-cards/ASIC3

Boster Bio - Best Uses Of The ASIC3 Marker

Boster's gene informationgraphics can be a great starting point when you are trying to figure how to use your ASIC3 data. The results can be used for special samples, species, and applications. You can use this product credit program to make your life easier. You can read more about the ASIC3 Marker to learn more!

Hommeric human ASIC3 channels are expressed by Xenopus oocytes

We found that Xenopus oocytes contained homomeric rat ASIC3 channels and homomeric rat A2x2. The physiology, pH sensitivities, and distribution of the ASIC3 channels in Xenopus oocytes and the P2x2 construct in rat are very similar. They colocalize within sensory neurons and coexpress the hASIC3 subunits and P2x2 subunits.

We tested messenger RNA in live cells to determine the functional differences between the two types. We used a Gibco-BRL mRNA targeting hASlC3 and expressed it in Xenopus oocytes with a pcDNA3 gene vector. These oocytes had to be cultured for two-three days at 19 and 15% C. We used a GeneClamp500 amplifier at a holding potential of -50 mV.

Xenopus oocytes do not express human ASIC3 cells. B. marinus oocytes are more efficient at expressing human amino acid transporters, but have leaky membranes. Axolotl oocytes lack the Ca2+ activated chloride channel, which was used in cloning Xenopus oocytes. Ambystoma is not a popular model system for studies on human ionchannel function.

ASIC channel Stoichiometry reveals a complex pharmaceuticalcology. Opioids reduce transient peak currents of DRG neurons while opioids canentiate a greater percentage of LIG group neurons. Recent studies have revealed the complex and varied pharmacological characteristics of ASIC channels. We have also found that nocistatin activates homomeric human ASIC channels in Xenopus oocytes.

ASIC3 subunits are associated with P2X ATP -gated channels in adult human beings. ASIC channels have proton-sensing abilities that are linked to their physiological functions in Ca2+ signaling, pH regulation, and the generation of an Ultradian oscillator. These properties may be crucial for human sensory neuron development. It is not yet known if they play a role in the development of nociceptive sensory neurons.

ASIC3 also exists in the ASIC2A subunit. It also contains homomeric human ASIC3 ligand-gated channels and Pc1a. Both subunits share the same splice variant. These channels are very similar in their pH sensitivity. In addition, ASIC2A is highly similar in sensitivity, and ASIC2A and ASIC3 have homomeric human ASIC subunits.

Matriptase and trypsin reduce the peak pH 4.0 currents of ASIC1 and ASIC2 when incubated with the two subunits of the matriptase enzyme. However, matriptase has no effect on the peak current of ASIC2-injected Oocytes. We are now in a position to determine whether ASIC1 and ASIC2 have a functional role in Xenopus embryonic development.

Despite the differences in vitelline membrane structure and fluorescence, both species show similar fluorescence. This suggests that both Xenopus oocytes possess homomeric ASIC3 channels. These results indicate that X. borealis oocytes can be used as a heterologous system to express neuronal ion channels. This finding may help us to understand how the two species function differently.

Benefits of using ASIC3 Marker

The ASIC3 Marker can be used as a neuronal marker. It is expressed in different parts of the brain and is duplicative to the protein. The arrows indicate neurons expressing the ASIC3 marker. The protein's expression levels vary in different neurons and can range from zero up to five. Its expression is proportional in brain to number of DRGs but not completely duplicate. The amount ASIC3 expression per DRG varies between four to six mice.