Product Info Summary
SKU: | A11388 |
---|---|
Size: | 50 µl |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | IP, IHC, WB |
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Product info
Product Name
Anti-ASC2 (ASC-2/POP) PYDC1 Antibody
SKU/Catalog Number
A11388
Size
50 µl
Form
Liquid
Description
Boster Bio Anti-ASC2 (ASC-2/POP) PYDC1 Antibody (Catalog# A11388). Tested in IP, IHC, WB application(s). This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store the antibody at 4°C, stable for 6 months. For long-term storage, store at -20°C. Avoid repeated freeze and thaw cycles.
Cite This Product
Anti-ASC2 (ASC-2/POP) PYDC1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A11388)
Host
Rabbit
Contents
50 μl sera
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A full-length recombinant protein of ASC2 (ASC-2/POP)
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A11388 is reactive to PYDC1 in Human, Mouse, Rat
Applications
A11388 is guaranteed for IP, IHC, WB Boster Guarantee
Observed Molecular Weight
Calculated molecular weight
10107 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:1000-1:2000
IHC (paraffin): 1:1000-1:5000
IHC (frozen): Users should optimize
IP: 1:50-1:200
Validation Images & Assay Conditions
![A11388 Immunostaining Analysis 3 A11388 Immunostaining Analysis 3](https://www.bosterbio.com/media/catalog/product/A/1/A11388-Immunostaining-Analysis-3.jpg)
Click image to see more details
Immunhistochemical analysis of ASC2/POP1 expression in mouse spleen using primary antibody dilution ratio of 1:2000. A and A1, spleen at low and high magnification, respectively. ASC2/POP1 staining is seen in the granulocytes, whereas the lymph and megakaryocytes are negative.
![A11388 Immunostaining Analysis 2 A11388 Immunostaining Analysis 2](https://www.bosterbio.com/media/catalog/product/A/1/A11388-Immunostaining-Analysis-2.jpg)
Click image to see more details
Immunhistochemical analysis of ASC2/POP1 expression in mouse using primary antibody dilution ratio of 1:2000. A, prostate tissue from a male mouse prostatitis. B, kidney from a mouse with pyelonephritis. A1 and B1, high magnification of A and B, respectively. ASC2/POP1 staining is seen in the granulocytes.
![A11388 Western Blotting Analysis 1 A11388 Western Blotting Analysis 1](https://www.bosterbio.com/media/catalog/product/A/1/A11388-Western-Blotting-Analysis-1.jpg)
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Western blot analysis of ASC2/POP1 using primary antibody dilution ratio of 1:2000. HEK-293 cells were transiently transfected with plasmids encoding ASC2, POP1 or a negative control plasmid. ASC2 and POP1 were detected at the expected 12 kDa molecular weight.
Protein Target Info & Infographic
Gene/Protein Information For PYDC1 (Source: Uniprot.org, NCBI)
Gene Name
PYDC1
Full Name
Pyrin domain-containing protein 1
Weight
10107 MW
Alternative Names
ASC2PAAD-only protein 1; ASCI; POP1pyrin domain-containing protein 1; PYC1; PYD (pyrin domain) containing 1; pyrin domain containing 1; pyrin-domain containing protein 1; Pyrin-only protein 1 PYDC1 ASC2, POP1, PYC1, cPOP1 pyrin domain containing 1 pyrin domain-containing protein 1|PAAD-only protein 1|PYD (pyrin domain) containing 1|cellular POP1|pyrin-only protein 1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on PYDC1, check out the PYDC1 Infographic
![PYDC1 infographic](/media/images/gene-infographic-example.jpg)
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for PYDC1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-ASC2 (ASC-2/POP) PYDC1 Antibody (A11388)
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No publications found for A11388
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Customer Q&As
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5 Customer Q&As for Anti-ASC2 (ASC-2/POP) PYDC1 Antibody
Question
I have a question about product A11388, anti-ASC2 (ASC-2/POP) antibody . I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-11-07
Answer
We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A11388 anti-ASC2 (ASC-2/POP) antibody , we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-11-07
Question
Is this A11388 anti-ASC2 (ASC-2/POP) antibody reactive to the isotypes of PYDC1?
S. Yang
Verified customer
Asked: 2019-01-11
Answer
The immunogen of A11388 anti-ASC2 (ASC-2/POP) antibody is A full-length recombinant protein of ASC2 (ASC-2/POP). Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-01-11
Question
We are currently using anti-ASC2 (ASC-2/POP) antibody A11388 for rat tissue, and we are content with the IP results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on primate tissues as well?
Verified Customer
Verified customer
Asked: 2018-12-21
Answer
The anti-ASC2 (ASC-2/POP) antibody (A11388) has not been tested for cross reactivity specifically with primate tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in primate you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2018-12-21
Question
Would anti-ASC2 (ASC-2/POP) antibody A11388 work for WB with cerebellum?
T. Krishna
Verified customer
Asked: 2018-11-16
Answer
According to the expression profile of cerebellum, PYDC1 is highly expressed in cerebellum. So, it is likely that anti-ASC2 (ASC-2/POP) antibody A11388 will work for WB with cerebellum.
Boster Scientific Support
Answered: 2018-11-16
Question
See attached the WB image, lot number and protocol we used for cerebellum using anti-ASC2 (ASC-2/POP) antibody A11388. Please let me know if you require anything else.
R. Mangal
Verified customer
Asked: 2015-01-12
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2015-01-12