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Product Info Summary
| SKU: | M00071 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Rat |
| Host: | Rabbit |
| Application: | IP, WB |
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Product info
Product Name
Anti-Aromatase CYP19A1 Rabbit Monoclonal Antibody
SKU/Catalog Number
M00071
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-Aromatase CYP19A1 Rabbit Monoclonal Antibody catalog # M00071. Tested in WB, IP applications. This antibody reacts with Human, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Aromatase CYP19A1 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00071)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
AABB-3
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human Aromatase
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M00071 is reactive to CYP19A1 in Human, Rat
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
25 kDa
Calculated molecular weight
57883 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00071 is guaranteed for IP, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IP 1:50
Validation Images & Assay Conditions
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All lanes use the Antibody at 1:2K dilution for 1 hour at room temperature.
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All lanes use the Antibody at 1:2K dilution for 1 hour at room temperature.
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All lanes use the Antibody at 1:2K dilution for 1 hour at room temperature.
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Western blot analysis of Aromatase expression in rat brain lysate.
Click image to see more details
All lanes use the Antibody at 1:2K dilution for 1 hour at room temperature.
Protein Target Info & Infographic
Gene/Protein Information For CYP19A1 (Source: Uniprot.org, NCBI)
Gene Name
CYP19A1
Full Name
Aromatase
Weight
57883 MW
Superfamily
cytochrome P450 family
Alternative Names
Aromatase;1.14.14.14;CYPXIX;Cytochrome P-450AROM;Cytochrome P450 19A1;Estrogen synthase;CYP19A1;ARO1, CYAR, CYP19; CYP19A1 ARO, ARO1, CPV1, CYAR, CYP19, CYPXIX, P-450AROM cytochrome P450 family 19 subfamily A member 1 aromatase|cytochrome P-450AROM|cytochrome P450 19A1|cytochrome P450, family 19, subfamily A, polypeptide 1|cytochrome P450, subfamily XIX (aromatization of androgens)|estrogen synthase|estrogen synthetase|flavoprotein-linked monooxygenase|microsomal monooxygenase
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CYP19A1, check out the CYP19A1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CYP19A1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Aromatase CYP19A1 Rabbit Monoclonal Antibody (M00071)
Hello CJ!
M00071 has been cited in 3 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Electroacupuncture enhances extragonadal aromatization in ovariectomized rats
Extragonadal aromatization increases with time after ovariectomy in rats
Zhang D, Yang B, Zou W, Lu X, Xiong M, Wu L, Wang J, Gao J, Xu S, Zou T. Biomed Res Int. 2013;2013:301540. Doi: 10.1155/2013/301540. Epub 2012 Dec 26. Estradiol Synthesis And Release In Cultured Female Rat Bone Marrow Stem Cells.
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Customer Reviews
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Customer Q&As
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15 Customer Q&As for Anti-Aromatase CYP19A1 Rabbit Monoclonal Antibody
Question
Is there a BSA free version of anti-Aromatase Rabbit Monoclonal antibody M00071 available?
A. Krishna
Verified customer
Asked: 2020-02-18
Answer
I appreciate your recent telephone inquiry. I can confirm that some lots of this anti-Aromatase Rabbit Monoclonal antibody M00071 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2020-02-18
Question
Is a blocking peptide available for product anti-Aromatase Rabbit Monoclonal antibody (M00071)?
Verified Customer
Verified customer
Asked: 2020-02-12
Answer
We do provide the blocking peptide for product anti-Aromatase Rabbit Monoclonal antibody (M00071). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2020-02-12
Question
We have been able to see staining in rat chorionic villus. Any tips? Is anti-Aromatase Rabbit Monoclonal antibody supposed to stain chorionic villus positively?
Verified Customer
Verified customer
Asked: 2020-01-09
Answer
From literature chorionic villus does express CYP19A1. From Uniprot.org, CYP19A1 is expressed in chorionic villus, placenta, ovary placenta, among other tissues. Regarding which tissues have CYP19A1 expression, here are a few articles citing expression in various tissues:
Ovary, and Placenta, Pubmed ID: 15489334
Placenta, Pubmed ID: 2541021, 2691883, 2973313, 3018730, 3964273
Boster Scientific Support
Answered: 2020-01-09
Question
Please see the WB image, lot number and protocol we used for placenta using anti-Aromatase Rabbit Monoclonal antibody M00071. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-12-06
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-12-06
Question
I have a question about product M00071, anti-Aromatase Rabbit Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
T. Miller
Verified customer
Asked: 2019-11-26
Answer
We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M00071 anti-Aromatase Rabbit Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-11-26
Question
I was wanting to use your anti-Aromatase Rabbit Monoclonal antibody for WB for rat placenta on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for rat placenta identification?
Verified Customer
Verified customer
Asked: 2019-10-03
Answer
You can see on the product datasheet, M00071 anti-Aromatase Rabbit Monoclonal antibody has been validated for IP, WB on human, rat tissues. We have an innovator award program that if you test this antibody and show it works in rat placenta in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-10-03
Question
Would anti-Aromatase Rabbit Monoclonal antibody M00071 work for WB with placenta?
Verified Customer
Verified customer
Asked: 2019-08-22
Answer
According to the expression profile of placenta, CYP19A1 is highly expressed in placenta. So, it is likely that anti-Aromatase Rabbit Monoclonal antibody M00071 will work for WB with placenta.
Boster Scientific Support
Answered: 2019-08-22
Question
I am looking for to test anti-Aromatase Rabbit Monoclonal antibody M00071 on rat placenta for research purposes, then I may be interested in using anti-Aromatase Rabbit Monoclonal antibody M00071 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-07-19
Answer
The products we sell, including anti-Aromatase Rabbit Monoclonal antibody M00071, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-07-19
Question
My team were well pleased with the WB result of your anti-Aromatase Rabbit Monoclonal antibody. However we have seen positive staining in chorionic villus endoplasmic reticulum membrane using this antibody. Is that expected? Could you tell me where is CYP19A1 supposed to be expressed?
Verified Customer
Verified customer
Asked: 2019-07-16
Answer
Based on literature, chorionic villus does express CYP19A1. Generally CYP19A1 expresses in endoplasmic reticulum membrane. Regarding which tissues have CYP19A1 expression, here are a few articles citing expression in various tissues:
Ovary, and Placenta, Pubmed ID: 15489334
Placenta, Pubmed ID: 2541021, 2691883, 2973313, 3018730, 3964273
Boster Scientific Support
Answered: 2019-07-16
Question
Thanks for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for placenta using anti-Aromatase Rabbit Monoclonal antibody M00071. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2018-12-19
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-12-19
Question
Is this M00071 anti-Aromatase Rabbit Monoclonal antibody reactive to the isotypes of CYP19A1?
Verified Customer
Verified customer
Asked: 2018-05-29
Answer
The immunogen of M00071 anti-Aromatase Rabbit Monoclonal antibody is A synthesized peptide derived from human Aromatase. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2018-05-29
Question
I see that the anti-Aromatase Rabbit Monoclonal antibody M00071 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2017-09-07
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2017-09-07
Question
We have tried in the past anti-Aromatase Rabbit Monoclonal antibody for WB on chorionic villus in a previous experiment. I am using human, and We want to use the antibody for IP next. I would like examining chorionic villus as well as ovary placenta in our next experiment. Could you please give me some suggestion on which antibody would work the best for IP?
N. Walker
Verified customer
Asked: 2017-04-18
Answer
I looked at the website and datasheets of our anti-Aromatase Rabbit Monoclonal antibody and I see that M00071 has been tested on human in both WB and IP. Thus M00071 should work for your application. Our Boster satisfaction guarantee will cover this product for IP in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for IP detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2017-04-18
Question
We are currently using anti-Aromatase Rabbit Monoclonal antibody M00071 for human tissue, and we are content with the IP results. The species of reactivity given in the datasheet says human, rat. Is it likely that the antibody can work on canine tissues as well?
C. Huang
Verified customer
Asked: 2016-10-21
Answer
The anti-Aromatase Rabbit Monoclonal antibody (M00071) has not been validated for cross reactivity specifically with canine tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in canine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2016-10-21
Question
Will M00071 anti-Aromatase Rabbit Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
M. Wu
Verified customer
Asked: 2013-10-07
Answer
You can see on the product datasheet, M00071 anti-Aromatase Rabbit Monoclonal antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2013-10-07
