Product Info Summary
SKU: | A10017 |
---|---|
Size: | 100 μg/vial |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | IHC, WB |
Customers Who Bought This Also Bought
Product info
Product Name
Anti-NDRG3 Antibody Picoband®
SKU/Catalog Number
A10017
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-NDRG3 Antibody Picoband® catalog # A10017. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-NDRG3 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A10017)
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human NDRG3 recombinant protein (Position: L181-R239). Human NDRG3 shares 93.1% and 94.9% amino acid (aa) sequence identity with mouse and rat NDRG3, respectively.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
A10017 is reactive to NDRG3 in Human, Mouse, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
41 kDa
Calculated molecular weight
41409 MW
Background of Ndrg3
In studies of the NDRG gene family in the human, novel mouse sequences of Ndr2 and Ndr3 were used to search the human genome databases. This confirmed the existence of a human NDRG3 gene, homologous to mouse Ndr3. The 3 known human NDRG proteins show considerable homology, with 54% amino acid sequence identity between NDRG1 and NDRG2, 67% between NDRG1 and NDRG3, and 58% between NDRG2 and NDRG3. The NDRG3 gene was represented by 86 ESTs and a genomic clone from chromosome 20q11.21-q11.23. This provisional chromosomal localization was confirmed by electronic PCR.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A10017 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Mouse, Rat, By Heat
Western blot, 0.1-0.5μg/ml, Human
Positive Control
WB: COLO320 whole cell
IHC: rat brain tissue, human mammary cancer tissue, human intestinal cancer tissue, mouse brain tissue
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of NDRG3 using anti-NDRG3 antibody (A10017).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: COLO320 whole cell lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NDRG3 antigen affinity purified polyclonal antibody (Catalog # A10017) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NDRG3 at approximately 41KD. The expected band size for NDRG3 is at 41KD.
Click image to see more details
Figure 3. IHC analysis of NDRG3 using anti-NDRG3 antibody (A10017).
NDRG3 was detected in paraffin-embedded section of rat brain tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-NDRG3 Antibody (A10017) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Click image to see more details
Figure 5. IHC analysis of NDRG3 using anti-NDRG3 antibody (A10017).
NDRG3 was detected in paraffin-embedded section of human mammary cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-NDRG3 Antibody (A10017) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Click image to see more details
Figure 4. IHC analysis of NDRG3 using anti-NDRG3 antibody (A10017).
NDRG3 was detected in paraffin-embedded section of human intestinal cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-NDRG3 Antibody (A10017) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Click image to see more details
Figure 2. IHC analysis of NDRG3 using anti-NDRG3 antibody (A10017).
NDRG3 was detected in paraffin-embedded section of mouse brain tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-NDRG3 Antibody (A10017) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Protein Target Info & Infographic
Gene/Protein Information For NDRG3 (Source: Uniprot.org, NCBI)
Gene Name
NDRG3
Full Name
Protein NDRG3
Weight
41409 MW
Superfamily
NDRG family
Alternative Names
Protein NDRG3;N-myc downstream-regulated gene 3 protein;NDRG3; Ndrg3|4833415O14Rik, AI747193, Nd, Ndr3|N-myc downstream regulated gene 3|protein NDRG3|N-myc downstream regulated 3|N-myc downstream-regulated gene 3 protein|protein Ndr3
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on NDRG3, check out the NDRG3 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for NDRG3: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-NDRG3 Antibody Picoband® (A10017)
Hello CJ!
No publications found for A10017
*Do you have publications using this product? Share with us and receive a reward. Ask us for more details.
Recommended Resources
Here are featured tools and databases that you might find useful.
- Boster's Pathways Library
- Protein Databases
- Bioscience Research Protocol Resources
- Data Processing & Analysis Software
- Photo Editing Software
- Scientific Literature Resources
- Research Paper Management Tools
- Molecular Biology Software
- Primer Design Tools
- Bioinformatics Tools
- Phylogenetic Tree Analysis
Customer Reviews
Have you used Anti-NDRG3 Antibody Picoband®?
Submit a review and receive an Amazon gift card.
- $30 for a review with an image
0 Reviews For Anti-NDRG3 Antibody Picoband®
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question
7 Customer Q&As for Anti-NDRG3 Antibody Picoband®
Question
My question regarding product A10017, anti-NDRG3 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-11-19
Answer
We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A10017 anti-NDRG3 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-11-19
Question
Is this A10017 anti-NDRG3 antibody reactive to the isotypes of NDRG3?
Verified Customer
Verified customer
Asked: 2019-06-28
Answer
The immunogen of A10017 anti-NDRG3 antibody is E.coli-derived human NDRG3 recombinant protein (Position: L181-R239). Human NDRG3 shares 93.1% and 94.9% amino acid (aa) sequence identity with mouse and rat NDRG3, respectively. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-06-28
Question
I see that the anti-NDRG3 antibody A10017 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-03-21
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2019-03-21
Question
Please see the WB image, lot number and protocol we used for cervix carcinoma using anti-NDRG3 antibody A10017. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-01-01
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-01-01
Question
I was wanting to use your anti-NDRG3 antibody for WB for mouse cervix carcinoma on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for mouse cervix carcinoma identification?
Verified Customer
Verified customer
Asked: 2018-04-04
Answer
It shows on the product datasheet, A10017 anti-NDRG3 antibody has been validated for IHC, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in mouse cervix carcinoma in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2018-04-04
Question
Is there a BSA free version of anti-NDRG3 antibody A10017 available?
Verified Customer
Verified customer
Asked: 2018-01-18
Answer
Thank you for your recent telephone inquiry. I can confirm that some lots of this anti-NDRG3 antibody A10017 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2018-01-18
Question
We are currently using anti-NDRG3 antibody A10017 for human tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on goat tissues as well?
A. Huang
Verified customer
Asked: 2016-06-01
Answer
The anti-NDRG3 antibody (A10017) has not been validated for cross reactivity specifically with goat tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in goat you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2016-06-01