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Key=meta_title, value=Anti-NDRG3 Antibody Picoband® | Bosterbio
Key=meta_description, value=Boster Bio Anti-NDRG3 Antibody Picoband® catalog # A10017. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat. Supplied as 100μg/vial in Lyophilized form antibody.
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Key=image_label, value=Figure 1. Western blot analysis of NDRG3 using anti-NDRG3 antibody (A10017).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of
Key=small_image_label, value=Figure 1. Western blot analysis of NDRG3 using anti-NDRG3 antibody (A10017).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of
Key=thumbnail_label, value=Figure 1. Western blot analysis of NDRG3 using anti-NDRG3 antibody (A10017).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of
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Key=size, value=100 μg/vial
Key=protein_name, value=Protein NDRG3
Key=gene_full_name, value=Protein NDRG3
Key=uniprot_id, value=Q9UGV2
Key=reconstitution, value=Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Key=sensitivity
Key=applications, value=IHC, WB
Key=reactivity, value=Human, Mouse, Rat
Key=form, value=Lyophilized
Key=concentration, value=Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Key=cross_reactivity, value=No cross-reactivity with other proteins
Key=recommended_detection_systems, value=Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P).
Key=predicted_reactivity
Key=source_company, value=Boster A10017
Key=isotype, value=Rabbit IgG
Key=molecular_weight, value=41409 MW
Key=sample_size_available, value=30ug for $99, contact us for details
Key=related_product_skus, value=B0001-5,AR1156,AR0146
Key=product_category, value=Primary Antibodies
Key=research_category, value=Cancer, Cell Biology, Cell Cycle, Cell Differentiation, Tumor Biomarkers
Key=clonality, value=Polyclonal
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Key=description, value=Boster Bio Anti-NDRG3 Antibody Picoband® catalog # A10017. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Key=short_description, value=Boster Bio Anti-NDRG3 Antibody Picoband® catalog # A10017. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Key=meta_keyword, value=Anti-NDRG3 Antibody Picoband®
Key=contents, value=Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Key=background, value=In studies of the NDRG gene family in the human, novel mouse sequences of Ndr2 and Ndr3 were used to search the human genome databases. This confirmed the existence of a human NDRG3 gene, homologous to mouse Ndr3. The 3 known human NDRG proteins show considerable homology, with 54% amino acid sequence identity between NDRG1 and NDRG2, 67% between NDRG1 and NDRG3, and 58% between NDRG2 and NDRG3. The NDRG3 gene was represented by 86 ESTs and a genomic clone from chromosome 20q11.21-q11.23. This provisional chromosomal localization was confirmed by electronic PCR.
Key=gene_name, value=NDRG3
Key=synonyms, value=Protein NDRG3;N-myc downstream-regulated gene 3 protein;NDRG3;
Key=application_details, value=Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Mouse, Rat, By Heat
Western blot, 0.1-0.5μg/ml, Human

Key=subcellular_localization, value= Membrane ; Single-pass type I membrane protein .
Key=tissue_specificity, value= Ubiquitous. Highly expressed in brain. .
Key=protein_function, value= Promotes the growth of epithelial cells. May stimulate the phosphorylation of EGFR and mitogen-activated protein kinases.
Key=application_notes, value=Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.
Key=competitor_equivalent_skus, value=sc 19470|sc 19471|sc 514561
Key=immunogen, value=E.coli-derived human NDRG3 recombinant protein (Position: L181-R239). Human NDRG3 shares 93.1% and 94.9% amino acid (aa) sequence identity with mouse and rat NDRG3, respectively.
Key=purification, value=Immunogen affinity purified.
Key=storage, value=Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Key=custom_attribute_1, value=41 kDa
Key=custom_attribute_2, value=WB: COLO320 whole cell
IHC: rat brain tissue, human mammary cancer tissue, human intestinal cancer tissue, mouse brain tissue
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Anti-NDRG3 Antibody Picoband®

Ndrg3 antibody

Boster Bio Anti-NDRG3 Antibody Picoband® catalog # A10017. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Product Info Summary

SKU: A10017
Size: 100 μg/vial
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: IHC, WB

Product Name

Anti-NDRG3 Antibody Picoband®

View all Ndrg3 Antibodies

SKU/Catalog Number

A10017

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-NDRG3 Antibody Picoband® catalog # A10017. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-NDRG3 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A10017)

Host

Rabbit

Contents

Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E.coli-derived human NDRG3 recombinant protein (Position: L181-R239). Human NDRG3 shares 93.1% and 94.9% amino acid (aa) sequence identity with mouse and rat NDRG3, respectively.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins

Reactive Species

A10017 is reactive to NDRG3 in Human, Mouse, Rat

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Observed Molecular Weight

41 kDa

Calculated molecular weight

41409 MW

Background of Ndrg3

In studies of the NDRG gene family in the human, novel mouse sequences of Ndr2 and Ndr3 were used to search the human genome databases. This confirmed the existence of a human NDRG3 gene, homologous to mouse Ndr3. The 3 known human NDRG proteins show considerable homology, with 54% amino acid sequence identity between NDRG1 and NDRG2, 67% between NDRG1 and NDRG3, and 58% between NDRG2 and NDRG3. The NDRG3 gene was represented by 86 ESTs and a genomic clone from chromosome 20q11.21-q11.23. This provisional chromosomal localization was confirmed by electronic PCR.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

View more details

Applications

A10017 is guaranteed for IHC, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Mouse, Rat, By Heat
Western blot, 0.1-0.5μg/ml, Human

Positive Control

WB: COLO320 whole cell
IHC: rat brain tissue, human mammary cancer tissue, human intestinal cancer tissue, mouse brain tissue

Validation Images & Assay Conditions

Gene/Protein Information For NDRG3 (Source: Uniprot.org, NCBI)

Gene Name

NDRG3

Full Name

Protein NDRG3

Weight

41409 MW

Superfamily

NDRG family

Alternative Names

Protein NDRG3;N-myc downstream-regulated gene 3 protein;NDRG3; Ndrg3|4833415O14Rik, AI747193, Nd, Ndr3|N-myc downstream regulated gene 3|protein NDRG3|N-myc downstream regulated 3|N-myc downstream-regulated gene 3 protein|protein Ndr3

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on NDRG3, check out the NDRG3 Infographic

NDRG3 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for NDRG3: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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7 Customer Q&As for Anti-NDRG3 Antibody Picoband®

Question

My question regarding product A10017, anti-NDRG3 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

Verified Customer

Verified customer

Asked: 2019-11-19

Answer

We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A10017 anti-NDRG3 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2019-11-19

Question

Is this A10017 anti-NDRG3 antibody reactive to the isotypes of NDRG3?

Verified Customer

Verified customer

Asked: 2019-06-28

Answer

The immunogen of A10017 anti-NDRG3 antibody is E.coli-derived human NDRG3 recombinant protein (Position: L181-R239). Human NDRG3 shares 93.1% and 94.9% amino acid (aa) sequence identity with mouse and rat NDRG3, respectively. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?

Boster Scientific Support

Answered: 2019-06-28

Question

I see that the anti-NDRG3 antibody A10017 works with WB, what is the protocol used to produce the result images on the product page?

Verified Customer

Verified customer

Asked: 2019-03-21

Answer

You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2019-03-21

Question

Please see the WB image, lot number and protocol we used for cervix carcinoma using anti-NDRG3 antibody A10017. Please let me know if you require anything else.

Verified Customer

Verified customer

Asked: 2019-01-01

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2019-01-01

Question

I was wanting to use your anti-NDRG3 antibody for WB for mouse cervix carcinoma on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for mouse cervix carcinoma identification?

Verified Customer

Verified customer

Asked: 2018-04-04

Answer

It shows on the product datasheet, A10017 anti-NDRG3 antibody has been validated for IHC, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in mouse cervix carcinoma in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2018-04-04

Question

Is there a BSA free version of anti-NDRG3 antibody A10017 available?

Verified Customer

Verified customer

Asked: 2018-01-18

Answer

Thank you for your recent telephone inquiry. I can confirm that some lots of this anti-NDRG3 antibody A10017 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.

Boster Scientific Support

Answered: 2018-01-18

Question

We are currently using anti-NDRG3 antibody A10017 for human tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on goat tissues as well?

A. Huang

Verified customer

Asked: 2016-06-01

Answer

The anti-NDRG3 antibody (A10017) has not been validated for cross reactivity specifically with goat tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in goat you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2016-06-01

Order DetailsPrice
A10017

100μg

$370
A10017-10ug

10μg sample (liquid)

$99
A10017-Biotin

100 μg Biotin conjugated

$570
A10017-Cy3

100 μg Cy3 conjugated

$570
A10017-Dylight488

100 μg Dylight488 conjugated

$570
A10017-Dylight550

100 μg Dylight550 conjugated

$570
A10017-Dylight594

100 μg Dylight594 conjugated

$570
A10017-FITC

100 μg FITC conjugated

$570
A10017-HRP

100 μg HRP conjugated

$570
A10017-APC

100 μg APC conjugated

$670
A10017-PE

100 μg PE conjugated

$670
A10017-iFluor647

100 μg iFluor647 conjugated

$670
A10017-carrier-free

Carrier Free

$370

More conjugates/formats

Free Secondary Antibody

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A10017
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00

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