Product Info Summary
SKU: | PA1511 |
---|---|
Size: | 100 μg/vial |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | WB |
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Product info
Product Name
Anti-Aquaporin 8/AQP8 Antibody
View all Aquaporin-8 Antibodies
SKU/Catalog Number
PA1511
BA3619-2 is an alternative SKU for this antibody, used in previous lots.
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Aquaporin 8/AQP8 Antibody catalog # PA1511. Tested in WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Aquaporin 8/AQP8 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1511)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2mg Na2HPO4, 0.05 mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the N-terminus of human Aquaporin 8.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PA1511 is reactive to AQP8 in Human, Mouse, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
27 kDa
Calculated molecular weight
27381 MW
Background of Aquaporin-8
AQP8, also known as Aquaporin-8, is a protein that in humans is encoded by the AQP8 gene. Aquaporin 8 (AQP8) is a water channel protein. Aquaporins are a family of small integral membrane proteins related to the major intrinsic protein (MIP or AQP0). Aquaporin 8 mRNA is found in pancreas and colon but not other tissues. The AQP8 gene contains 6 exons. It is mapped to chromosome 16p12. Northern blot analysis of human tissues detected a 1.35-kb AQP8 mRNA only in pancreas and colon.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PA1511 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat
Positive Control
WB: human A431 whole cell, human PC-3 whole cell
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of AQP8 using anti-AQP8 antibody (PA1511).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human A431 whole cell lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-AQP8 antigen affinity purified polyclonal antibody (Catalog # PA1511) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for AQP8 at approximately 27KD. The expected band size for AQP8 is at 27KD.
Click image to see more details
Figure 2. Western blot analysis of AQP8 using anti-AQP8 antibody (PA1511).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human PC-3 whole cell lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-AQP8 antigen affinity purified polyclonal antibody (Catalog # PA1511) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for AQP8 at approximately 27KD. The expected band size for AQP8 is at 27KD.
Protein Target Info & Infographic
Gene/Protein Information For AQP8 (Source: Uniprot.org, NCBI)
Gene Name
AQP8
Full Name
Aquaporin-8
Weight
27381 MW
Superfamily
MIP/aquaporin (TC 1.A.8) family
Alternative Names
Aquaporin-8;AQP-8;AQP8; AQP8 AQP-8 aquaporin 8 aquaporin-8
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on AQP8, check out the AQP8 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for AQP8: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Aquaporin 8/AQP8 Antibody (PA1511)
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1 Customer Q&As for Anti-Aquaporin 8/AQP8 Antibody
Question
We are currently using anti-Aquaporin 8/AQP8 antibody PA1511 for human tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human. Is it true that the antibody can work on pig tissues as well?
B. Jackson
Verified customer
Asked: 2018-12-14
Answer
The anti-Aquaporin 8/AQP8 antibody (PA1511) has not been tested for cross reactivity specifically with pig tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in pig you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2018-12-14