Product Info Summary
SKU: | M00865 |
---|---|
Size: | 100 μl |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | IF, IHC, ICC, WB |
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Product info
Product Name
Anti-AQP1 Rabbit Monoclonal Antibody
View all Aquaporin 1/AQP1 Antibodies
SKU/Catalog Number
M00865
BM5035 is an alternative SKU for this antibody, used in previous lots.
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-AQP1 Rabbit Monoclonal Antibody catalog # M00865. Tested in WB, IHC, ICC/IF applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-AQP1 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00865)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
AAEE-1
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human AQP1
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M00865 is reactive to AQP1 in Human, Mouse, Rat
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
35 kDa
Calculated molecular weight
28526 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00865 is guaranteed for IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:50-1:200
ICC/IF 1:50-1:200
Positive Control
WB: Human fetal kidney, Human fetal lung
IHC: human breast tissue
Validation Images & Assay Conditions
Click image to see more details
Western blot analysis of AQP1 expression in (1) Human fetal kidney lysate; (2) Human fetal lung lysate.
Click image to see more details
Immunohistochemical analysis of paraffin-embedded human breast, using AQP1 Antibody.
Protein Target Info & Infographic
Gene/Protein Information For AQP1 (Source: Uniprot.org, NCBI)
Gene Name
AQP1
Full Name
Aquaporin-1
Weight
28526 MW
Superfamily
MIP/aquaporin (TC 1.A.8) family
Alternative Names
Aquaporin-1;AQP-1;Aquaporin-CHIP;Urine water channel;Water channel protein for red blood cells and kidney proximal tubule;AQP1;CHIP28; AQP1 AQP-CHIP, CHIP28, CO aquaporin 1 (Colton blood group) aquaporin-1|Colton blood group |aquaporin 1 (channel-forming integral protein, 28kDa, CO blood group)|aquaporin 1, Colton blood group |aquaporin-CHIP|channel-like integral membrane protein, 28-kDa|urine water channel|water channel protein for red blood cells and kidney proximal tubule
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on AQP1, check out the AQP1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for AQP1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-AQP1 Rabbit Monoclonal Antibody (M00865)
Hello CJ!
M00865 has been cited in 6 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Dynamic expression and roles of sequestome‑1/p62 in LPS‑induced acute kidney injury in mice
Sunitinib treatment enhances metastasis of innately drug-resistant breast tumors
Effect of selective inhibition of aquaporin 1 on chemotherapy sensitivity of J82 human bladder cancer cells
Effects of dexmedetomidine on the protection of hyperoxia-induced lung injury in newborn rats
Dynamic expression and roles of sequestome-1/p62 in LPS-induced acute kidney injury in mice
Huo J, Liu J, Wang J, Zhang Y, Wang C, Yang Y, Sun W, Xu S. Neural Regen Res. 2012 Aug 5;7(22):1729-35. Doi: 10.3969/J.Issn.1673-5374.2012.22.008. Early Hyperbaric Oxygen Therapy Inhibits Aquaporin 4 And Adrenocorticotropic Hormone Expression In T...
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Customer Reviews
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Customer Q&As
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15 Customer Q&As for Anti-AQP1 Rabbit Monoclonal Antibody
Question
Is a blocking peptide available for product anti-AQP1 Rabbit Monoclonal antibody (M00865)?
Verified Customer
Verified customer
Asked: 2020-04-22
Answer
We do provide the blocking peptide for product anti-AQP1 Rabbit Monoclonal antibody (M00865). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2020-04-22
Question
I have a question about product M00865, anti-AQP1 Rabbit Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2020-04-14
Answer
We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M00865 anti-AQP1 Rabbit Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2020-04-14
Question
We have observed staining in rat mesangial cell. What should we do? Is anti-AQP1 Rabbit Monoclonal antibody supposed to stain mesangial cell positively?
Verified Customer
Verified customer
Asked: 2020-02-20
Answer
From what I have seen in literature mesangial cell does express AQP1. From what I have seen in Uniprot.org, AQP1 is expressed in right lung, retinal pigment epithelium, uterus, mesangial cell, brain, articular cartilage, among other tissues. Regarding which tissues have AQP1 expression, here are a few articles citing expression in various tissues:
Articular cartilage, Pubmed ID: 2007592, 1373524
Brain, Pubmed ID: 15489334
Mesangial cell, Pubmed ID: 14702039
Retinal pigment epithelium, Pubmed ID: 8703970
Uterus, Pubmed ID: 7517253
Boster Scientific Support
Answered: 2020-02-20
Question
See below the WB image, lot number and protocol we used for uterus using anti-AQP1 Rabbit Monoclonal antibody M00865. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-12-19
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-12-19
Question
I see that the anti-AQP1 Rabbit Monoclonal antibody M00865 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-11-28
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2019-11-28
Question
My colleagues were happy with the WB result of your anti-AQP1 Rabbit Monoclonal antibody. However we have been able to see positive staining in articular cartilage cell membrane using this antibody. Is that expected? Could you tell me where is AQP1 supposed to be expressed?
Verified Customer
Verified customer
Asked: 2019-09-02
Answer
From literature, articular cartilage does express AQP1. Generally AQP1 expresses in cell membrane. Regarding which tissues have AQP1 expression, here are a few articles citing expression in various tissues:
Articular cartilage, Pubmed ID: 2007592, 1373524
Brain, Pubmed ID: 15489334
Mesangial cell, Pubmed ID: 14702039
Retinal pigment epithelium, Pubmed ID: 8703970
Uterus, Pubmed ID: 7517253
Boster Scientific Support
Answered: 2019-09-02
Question
Do you have a BSA free version of anti-AQP1 Rabbit Monoclonal antibody M00865 available?
Verified Customer
Verified customer
Asked: 2019-06-24
Answer
I appreciate your recent telephone inquiry. I can confirm that some lots of this anti-AQP1 Rabbit Monoclonal antibody M00865 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-06-24
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for uterus using anti-AQP1 Rabbit Monoclonal antibody M00865. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2018-12-06
Answer
Thank you for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-12-06
Question
Is this M00865 anti-AQP1 Rabbit Monoclonal antibody reactive to the isotypes of AQP1?
Verified Customer
Verified customer
Asked: 2017-12-12
Answer
The immunogen of M00865 anti-AQP1 Rabbit Monoclonal antibody is A synthesized peptide derived from human AQP1. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2017-12-12
Question
We bought anti-AQP1 Rabbit Monoclonal antibody for WB on right lung in a previous project. I am using human, and We are going to use the antibody for IF next. We are interested in examining right lung as well as mesangial cell in our next experiment. Could you please give me some suggestion on which antibody would work the best for IF?
Verified Customer
Verified customer
Asked: 2017-09-21
Answer
I took a look at the website and datasheets of our anti-AQP1 Rabbit Monoclonal antibody and it seems that M00865 has been validated on human in both WB and IF. Thus M00865 should work for your application. Our Boster satisfaction guarantee will cover this product for IF in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for IF detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2017-09-21
Question
We need to test anti-AQP1 Rabbit Monoclonal antibody M00865 on human uterus for research purposes, then I may be interested in using anti-AQP1 Rabbit Monoclonal antibody M00865 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
P. Zhang
Verified customer
Asked: 2017-08-30
Answer
The products we sell, including anti-AQP1 Rabbit Monoclonal antibody M00865, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2017-08-30
Question
We are currently using anti-AQP1 Rabbit Monoclonal antibody M00865 for rat tissue, and we are happy with the IF results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on monkey tissues as well?
Verified Customer
Verified customer
Asked: 2017-06-30
Answer
The anti-AQP1 Rabbit Monoclonal antibody (M00865) has not been validated for cross reactivity specifically with monkey tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in monkey you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2017-06-30
Question
I was wanting to use your anti-AQP1 Rabbit Monoclonal antibody for WB for human uterus on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human uterus identification?
D. Dhar
Verified customer
Asked: 2015-08-11
Answer
As indicated on the product datasheet, M00865 anti-AQP1 Rabbit Monoclonal antibody has been validated for IF, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in human uterus in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2015-08-11
Question
Would M00865 anti-AQP1 Rabbit Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
E. Kulkarni
Verified customer
Asked: 2015-08-06
Answer
As indicated on the product datasheet, M00865 anti-AQP1 Rabbit Monoclonal antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2015-08-06
Question
Will anti-AQP1 Rabbit Monoclonal antibody M00865 work for WB with uterus?
J. Huang
Verified customer
Asked: 2015-03-17
Answer
According to the expression profile of uterus, AQP1 is highly expressed in uterus. So, it is likely that anti-AQP1 Rabbit Monoclonal antibody M00865 will work for WB with uterus.
Boster Scientific Support
Answered: 2015-03-17