Product Info Summary
SKU: | PA2094 |
---|---|
Size: | 100 μg/vial |
Reactive Species: | Mouse |
Host: | Rabbit |
Application: | WB |
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Product info
Product Name
Anti-Aquaporin 9/AQP9 Antibody Picoband®
View all Aquaporin-9 Antibodies
SKU/Catalog Number
PA2094
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Aquaporin 9/AQP9 Antibody catalog # PA2094. Tested in WB applications. This antibody reacts with Mouse. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Aquaporin 9/AQP9 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA2094)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of mouse Aquaporin 9.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PA2094 is reactive to Aqp9 in Mouse
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
31 kDa
Calculated molecular weight
31764 MW
Background of Aquaporin-9
Aquaporin-9 is a protein that in humans is encoded by the AQP9 gene. AQP9 encodes a 295-amino-acid protein with the amino acid sequence identity with AQP3 (48%), AQP7 (45%), and other aquaporins (approximately 30%), suggesting that AQP3, AQP7, and AQP9 belong to a subfamily of the aquaporin family. AQP9 is the major glycerol channel in mouse erythrocytes and suggest that this transport pathway may contribute to the virulence of intraerythrocytic stages of malarial infection.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PA2094 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Mouse
Positive Control
WB: Mouse Liver Tissue, Mouse Lung Tissue, Mouse Spleen Tissue, Mouse Testis Tissue, PC-12 Whole Cell
Validation Images & Assay Conditions
Click image to see more details
Anti-Aquaporin 9 antibody, PA2094, Western blotting
All lanes: Anti Aquaporin 9 (PA2094) at 0.5ug/ml
Lane 1: Mouse Liver Tissue Lysate at 50ug
Lane 2: Mouse Lung Tissue Lysate at 50ug
Lane 3: Mouse Spleen Tissue Lysate at 50ug
Lane 4: Mouse Testis Tissue Lysate at 50ug
Lane 5: PC-12 Whole Cell Lysate at 40ug
Predicted bind size: 31KD
Observed bind size: 31KD
Protein Target Info & Infographic
Gene/Protein Information For Aqp9 (Source: Uniprot.org, NCBI)
Gene Name
Aqp9
Full Name
Aquaporin-9
Weight
31764 MW
Superfamily
MIP/aquaporin (TC 1.A.8) family
Alternative Names
Aquaporin-9;AQP-9;Aquaglyceroporin-9;Aqp9; AQP9 AQP-9, HsT17287, SSC1, T17287 aquaporin 9 aquaporin-9|aquaglyceroporin-9|small solute channel 1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on Aqp9, check out the Aqp9 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for Aqp9: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Aquaporin 9/AQP9 Antibody Picoband® (PA2094)
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Customer Q&As
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6 Customer Q&As for Anti-Aquaporin 9/AQP9 Antibody Picoband®
Question
I see that the anti-Aquaporin 9/AQP9 antibody PA2094 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2020-03-26
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2020-03-26
Question
Will PA2094 anti-Aquaporin 9/AQP9 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-10-30
Answer
As indicated on the product datasheet, PA2094 anti-Aquaporin 9/AQP9 antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-10-30
Question
Is this PA2094 anti-Aquaporin 9/AQP9 antibody reactive to the isotypes of AQP9?
Verified Customer
Verified customer
Asked: 2019-08-08
Answer
The immunogen of PA2094 anti-Aquaporin 9/AQP9 antibody is A synthetic peptide corresponding to a sequence at the C-terminus of mouse Aquaporin 9(263-278aa VLFIQMHHSNPDPEVK). Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-08-08
Question
Do you have a BSA free version of anti-Aquaporin 9/AQP9 antibody PA2094 available?
Verified Customer
Verified customer
Asked: 2018-05-01
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-Aquaporin 9/AQP9 antibody PA2094 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2018-05-01
Question
Is a blocking peptide available for product anti-Aquaporin 9/AQP9 antibody (PA2094)?
K. Huang
Verified customer
Asked: 2016-01-20
Answer
We do provide the blocking peptide for product anti-Aquaporin 9/AQP9 antibody (PA2094). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2016-01-20
Question
We are currently using anti-Aquaporin 9/AQP9 antibody PA2094 for mouse tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says mouse. Is it true that the antibody can work on bovine tissues as well?
L. Baker
Verified customer
Asked: 2015-05-14
Answer
The anti-Aquaporin 9/AQP9 antibody (PA2094) has not been validated for cross reactivity specifically with bovine tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in bovine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2015-05-14