Click image to see more details
Product Info Summary
| SKU: | A02181 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-Aquaporin 3/Aqp3 Antibody Picoband®
View all Aquaporin-3 Antibodies
SKU/Catalog Number
A02181
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Aquaporin 3/Aqp3 Antibody Picoband® catalog # A02181. Tested in WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Aquaporin 3/Aqp3 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A02181)
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence in the middle region of mouse Aquaporin 3, different from the related human sequence by three amino acids, and from the related rat sequence by two amino acids.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A02181 is reactive to Aqp3 in Human, Mouse, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
32 kDa
Calculated molecular weight
31568 MW
Background of Aquaporin-3
This gene encodes the water channel protein aquaporin 3. Aquaporins are a family of small integral membrane proteins related to the major intrinsic protein, also known as aquaporin 0. Aquaporin 3 is localized at the basal lateral membranes of collecting duct cells in the kidney. In addition to its water channel function, aquaporin 3 has been found to facilitate the transport of nonionic small solutes such as urea and glycerol, but to a smaller degree. It has been suggested that water channels can be functionally heterogeneous and possess water and solute permeation mechanisms. Alternative splicing of this gene results in multiple transcript variants encoding different isoforms.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A02181 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Mouse, Rat, Human
Positive Control
WB: mouse kidney tissue, mouse brain tissue, rat kidney tissue, rat brain tissue
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of Aquaporin 3 using anti-Aquaporin 3 antibody (A02181).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: mouse kidney tissue lysates,
Lane 2: mouse brain tissue lysates,
Lane 3: rat kidney tissue lysates,
Lane 4: rat brain tissue lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Aquaporin 3 antigen affinity purified polyclonal antibody (Catalog # A02181) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Aquaporin 3 at approximately 32KD. The expected band size for Aquaporin 3 is at 32KD.
Protein Target Info & Infographic
Gene/Protein Information For Aqp3 (Source: Uniprot.org, NCBI)
Gene Name
Aqp3
Full Name
Aquaporin-3
Weight
31568 MW
Superfamily
MIP/aquaporin (TC 1.A.8) family
Alternative Names
Aquaporin-3;AQP-3;Aquaglyceroporin-3;Aqp3; AQP3 AQP-3, GIL aquaporin 3 (Gill blood group) aquaporin-3|aquaglyceroporin-3|aquaporin 3 (GIL blood group)
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on Aqp3, check out the Aqp3 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for Aqp3: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Aquaporin 3/Aqp3 Antibody Picoband® (A02181)
Hello CJ!
A02181 has been cited in 1 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Expression pattern of aquaporins in patients with primary nephrotic syndrome with edema
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Customer Q&As
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5 Customer Q&As for Anti-Aquaporin 3/Aqp3 Antibody Picoband®
Question
I see that the anti-Aquaporin 3/Aqp3 antibody A02181 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2020-04-27
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2020-04-27
Question
I was wanting to use to test anti-Aquaporin 3/Aqp3 antibody A02181 on mouse kidney for research purposes, then I may be interested in using anti-Aquaporin 3/Aqp3 antibody A02181 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-05-08
Answer
The products we sell, including anti-Aquaporin 3/Aqp3 antibody A02181, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-05-08
Question
I have a question about product A02181, anti-Aquaporin 3/Aqp3 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
L. Gonzalez
Verified customer
Asked: 2019-04-11
Answer
We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A02181 anti-Aquaporin 3/Aqp3 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-04-11
Question
Thanks for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for kidney using anti-Aquaporin 3/Aqp3 antibody A02181. Let me know if you need anything else.
A. Miller
Verified customer
Asked: 2018-10-02
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-10-02
Question
Is this A02181 anti-Aquaporin 3/Aqp3 antibody reactive to the isotypes of AQP3?
L. Collins
Verified customer
Asked: 2013-10-31
Answer
The immunogen of A02181 anti-Aquaporin 3/Aqp3 antibody is A synthetic peptide corresponding to a sequence in the middle region of mouse Aquaporin 3 (122-137aa LYYDAIWAFANNELFV), different from the related human sequence by three amino acids, and from the related rat sequence by two amino acids. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2013-10-31
