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Product Info Summary
| SKU: | PB9295 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-ANP/NPPA Antibody Picoband®
View all Atrial Natriuretic Peptide/ANP Antibodies
SKU/Catalog Number
PB9295
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-ANP/NPPA Antibody Picoband® catalog # PB9295. Tested in WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-ANP/NPPA Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9295)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human ANP, different from the related mouse and rat sequences by one amino acid.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PB9295 is reactive to NPPA in Human, Mouse, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
17 kDa
Calculated molecular weight
16708 MW
Background of Atrial Natriuretic Peptide/ANP
Atrial natriuretic peptide (ANP), also known as NPPA or PND, is a powerful vasodilator, and a protein (polypeptide) hormone secreted by heart muscle cells. This gene is mapped to 1p36.22. It is involved in the homeostatic control of body water, sodium, potassium and fat (adipose tissue). ANP is released by muscle cells in the upper chambers (atria) of the heart (atrial myocytes) in response to high blood volume. It acts to reduce the water, sodium and adipose loads on the circulatory system, thereby reducing blood pressure. ANP has exactly the opposite function of the aldosterone secreted by the zona glomerulosa in regard to its effect on sodium in the kidney – that is, aldosterone stimulates sodium retention and it generates sodium loss.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PB9295 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Mouse, Rat, Human
Positive Control
WB: Rat Cardiac Muscle Tissue, Mouse Cardiac Muscle Tissue
Validation Images & Assay Conditions
Click image to see more details
Anti-ANP Picoband antibody, PB9295, Western blotting
All lanes: Anti ANP (PB9295) at 0.5ug/ml
Lane 1: Rat Cardiac Muscle Tissue Lysate at 50ug
Lane 2: Mouse Cardiac Muscle Tissue Lysate at 50ug
Predicted bind size: 17KD
Observed bind size: 17KD
Protein Target Info & Infographic
Gene/Protein Information For NPPA (Source: Uniprot.org, NCBI)
Gene Name
NPPA
Full Name
Natriuretic peptides A
Weight
16708 MW
Superfamily
natriuretic peptide family
Alternative Names
Natriuretic peptides A;CDD-ANF;Cardiodilatin;CDD;Cardiodilatin-related peptide;CDP;Prepronatriodilatin;Atrial natriuretic factor;ANF;Atrial natriuretic peptide;ANP;NPPA;ANP, PND; NPPA ANF, ANP, ATFB6, ATRST2, CDD, CDD-ANF, CDP, PND natriuretic peptide A natriuretic peptides A|atrial natriuretic factor prohormone|atrial natriuretic peptide prohormone|atriopeptigen|atriopeptin|cardiodilatin-related peptide|cardionatrin|natriuretic peptide precursor A variant 1|preproANP|preproCDD-ANF|prepronatriodilatin|proANF|proANP
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on NPPA, check out the NPPA Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for NPPA: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-ANP/NPPA Antibody Picoband® (PB9295)
Hello CJ!
PB9295 has been cited in 1 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Localization of corin and atrial natriuretic peptide expression in human renal segments
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Customer Q&As
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6 Customer Q&As for Anti-ANP/NPPA Antibody Picoband®
Question
My question regarding product PB9295, anti-ANP/NPPA antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-11-01
Answer
We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free PB9295 anti-ANP/NPPA antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-11-01
Question
We are currently using anti-ANP/NPPA antibody PB9295 for rat tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on canine tissues as well?
Verified Customer
Verified customer
Asked: 2019-10-16
Answer
The anti-ANP/NPPA antibody (PB9295) has not been tested for cross reactivity specifically with canine tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in canine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-10-16
Question
Is this PB9295 anti-ANP/NPPA antibody reactive to the isotypes of NPPA?
Verified Customer
Verified customer
Asked: 2019-08-22
Answer
The immunogen of PB9295 anti-ANP/NPPA antibody is A synthetic peptide corresponding to a sequence at the C-terminus of human ANP(124-151aa SLRRSSCFGGRMDRIGAQSGLGCNSFRY), different from the related mouse and rat sequences by one amino acid. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-08-22
Question
I see that the anti-ANP/NPPA antibody PB9295 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-07-16
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2019-07-16
Question
Is PB9295 conjugated or not?
Verified customer
Asked: 2019-06-26
Answer
The Anti-ANP/NPPA Antibody Picoband™ (PB9295) is not conjugated.
Boster Scientific Support
Answered: 2019-06-26
Question
Does anti-ANP/NPPA antibody PB9295 work on dog WB with prostate?
Verified Customer
Verified customer
Asked: 2017-06-15
Answer
Our lab technicians have not validated anti-ANP/NPPA antibody PB9295 on dog. You can run a BLAST between dog and the immunogen sequence of anti-ANP/NPPA antibody PB9295 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated dog samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in dog prostate in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2017-06-15
