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Product Info Summary
| SKU: | EK1627 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Human |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, serum and plasma (heparin, EDTA). |
Product info
Product Name
Human NT-proANP/NPPA ELISA Kit PicoKine®
View all Atrial Natriuretic Peptide/ANP ELISA kits
SKU/Catalog Number
EK1627
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Human NT-proANP/NPPA ELISA Kit PicoKine® (96 Tests). Quantitate Human NPPA in cell culture supernatants, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Human NT-proANP/NPPA ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK1627)
Clonality of Antibodies
See Datasheet for details
Standard Protein
Expression system for standard: E.coli; Immunogen sequence: N26-R123
Sensitivity
<10 pg/ml
Assay Range
31.2 pg/ml - 2,000 pg/ml
Standard Dilution Instructions
See datasheet of EK1627 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK1627 is reactive to NPPA in Human samples
Validated Sample Types
cell culture supernatants, serum and plasma (heparin, EDTA).
Application Guarantee
EK1627 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of Atrial Natriuretic Peptide/ANP
Atrial natriuretic peptide (ANP), also known as NPPA or PND, is a powerful vasodilator, and a protein (polypeptide) hormone secreted by heart muscle cells. This gene is mapped to 1p36.22. It is involved in the homeostatic control of body water, sodium, potassium and fat (adipose tissue). ANP is released by muscle cells in the upper chambers (atria) of the heart (atrial myocytes) in response to high blood volume. It acts to reduce the water, sodium and adipose loads on the circulatory system, thereby reducing blood pressure. ANP has exactly the opposite function of the aldosterone secreted by the zona glomerulosa in regard to its effect on sodium in the kidney – that is, aldosterone stimulates sodium retention and it generates sodium loss.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK1627-CAP | Anti-Human NPPA Pre-coated 96-well strip microplate | 1 |
| EK1627-ST | Human NPPA Standard | 2 vials, 2 ng/tube |
| EK1627-DA | Human NPPA Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
Data Examples, Quality Control Data & Sample Dilution
Validation Standard Curve O.D. At 450nm
| Concentration (pg/ml) | 0 | 31.2 | 62.5 | 125 | 250 | 500 | 1000 | 2000 |
| O.D. | 0.094 | 0.158 | 0.228 | 0.376 | 0.669 | 1.101 | 1.812 | 2.014 |
Data Example Images
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Human NT-proANP/NPPA PicoKine ELISA Kit standard curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect Atrial Natriuretic Peptide/ANP, Dilution ratio of 1:10, concentration in serum and plasma is 0.77-45.96 ng/ml..
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human NT-proANP/NPPA ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 90 | 200 | 925 | 91 | 182 | 898 |
| Standard deviation | 4.23 | 13.8 | 52.72 | 5.46 | 15.83 | 66.45 |
| CV (%) | 4.7% | 6.9% | 5.7% | 6% | 8.7% | 7.4% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of Atrial Natriuretic Peptide/ANP in ELISA kits from four different production batches/lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 90 | 94 | 86 | 82 | 88 | 4.47 | 5% |
| Sample 2 | 200 | 217 | 235 | 206 | 214 | 13.31 | 6.2% |
| Sample 3 | 925 | 929 | 1045 | 879 | 944 | 61.25 | 6.4% |
Protein Target Info & Infographic
Gene/Protein Information For NPPA (Source: Uniprot.Org, NCBI)
Gene Name
NPPA
Full Name
Natriuretic peptides A
Weight
16.396kDa
Superfamily
natriuretic peptide family
Alternative Names
Natriuretic peptides A; CDD-ANF; Cardiodilatin; CDD; Cardiodilatin-related peptide; CDP; Prepronatriodilatin; Atrial natriuretic factor; ANF; Atrial natriuretic peptide; ANP; NPPA; ANP; PND NPPA ANF, ANP, ATFB6, ATRST2, CDD, CDD-ANF, CDP, PND natriuretic peptide A natriuretic peptides A|atrial natriuretic factor prohormone|atrial natriuretic peptide prohormone|atriopeptigen|atriopeptin|cardiodilatin-related peptide|cardionatrin|natriuretic peptide precursor A variant 1|preproANP|preproCDD-ANF|prepronatriodilatin|proANF|proANP
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on NPPA, check out the NPPA Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for NPPA: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Human NT-proANP/NPPA ELISA Kit PicoKine® (EK1627)
Hello CJ!
EK1627 has been cited in 1 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Effect of growth factors (BMP-4/7 & bFGF) on proliferation & osteogenic differentiation of bone marrow stromal cells
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Customer Reviews
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Customer Q&As
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8 Customer Q&As for Human NT-proANP/NPPA ELISA Kit PicoKine®
Question
Q: is there any online tool I can use to streamline the data analysis for my ELISA results?
Verified Customer
Verified customer
Asked: 2020-09-11
Answer
A: We have a web based ELISA curve fitting (4pl) and data analysis tool. Please give it a try: bosterbio.com/biology-research-tools/ELISA-data-analysis-online. You can also consult our article on ELISA data analysis: bosterbio.com/ELISA-data-analysis-instructions
Boster Scientific Support
Answered: 2020-09-11
Question
Q: how long can samples (cell cultures, serum, and plasma) be stored and still be stable for measuring ANP using the EK1627 Human ANP Picokine® ELISA Kit?
Verified Customer
Verified customer
Asked: 2020-08-16
Answer
A: Boster Bio does not evaluate sample stability. Variations in sample collection, processing, and storage may change the stabilityof samples. It is recommend to assay sample immediately after collection when possible, or aliquot into single use volumes and store samples frozen. limit repetitive freeze-thaw cycles with the stored samples in order to limit protein degradation.
Boster Scientific Support
Answered: 2020-08-16
Question
Q: Are Boster Bio recombinant proteins and antibodies sterile?
Verified Customer
Verified customer
Asked: 2020-07-19
Answer
A: although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is required for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.
Boster Scientific Support
Answered: 2020-07-19
Question
Q: can you tell me how to prepare cell lysates prepared for use in Picokine® ELISA kits?
Verified Customer
Verified customer
Asked: 2020-05-07
Answer
A: for those Picokine® ELISAs where cell or tissue lysate is a validated sample type, sample preparation instructions for lysate are included in the product insert. Components in lysate and lysis buffer can have an affect on immunoreactivity, so if lysate is not a validated sample type, care must be taken in sample preparation and validation.
Boster Scientific Support
Answered: 2020-05-07
Question
Q: What is the optimal O.D. value for ANP ELISA kit? I performed your ANP ELISA on serum samples. For my positive control, I received an O.D. value of 0.826, while my negative control received a value of 0.136. I obtained both of these controls from the ELISA kit, where your kit's typical data shows O.D. values much higher than my positive control and your background is lower. My samples O.D. values are around 0.225 and the highest is only 0.357. is it safe to say these samples contain ANP even though the O.D. values are not very high?
D. Davis
Verified customer
Asked: 2019-02-04
Answer
A: The absolute O.D. values may change according to incubation time. The more you incubate the higher the O.D. values are going to be. what you should focus on is whether your sample O.D. values are statistically significantly higher than your blank values. in the example mentioned, you could extend your development time in the substrate incubation step to obtain higher O.D. values, as long as your negative controls' O.D. values are not increasing faster in relation to your positive controls. normally, a sample with O.D. value 2 standard deviations higher than your negative controls can be considered positive. We calculate the sensitivity of this ELISA kit by converting cutoff O.D. value, calculated as the average of 20 negative controls plus 2 standard deviations of the 20 negative controls, into a concentration. in other words, when we claim this ANP ELISA kit to have sensitivity of 10pg/ml, that means the minimum amount of ANP that can be declared/interpreted as positive by the above standard is 10pg/ml.
Boster Scientific Support
Answered: 2019-02-04
Question
Q: we need your suggestion regarding the dilution ratio of serum samples for detection of ANP in Human plasma? I am trying to measure a few parameters and it requires 100ul of diluted samples for each well. We have limited sample volumes so we like to dilute as much as possible.
Verified Customer
Verified customer
Asked: 2018-10-21
Answer
A: unable to understand the physiological or pathological context of your samples we cannot recommend a dilution ratio without performing a pilot test with your samples. Here is how you can perform a pilot study on your own: perform a serial dilution of your samples on the ANP ELISA kit to make sure you have a linear ascending curve followed by a plateau, which signifies the samples saturating the detection limit of the kit. Then you can pick the dilution ratios from samples in the linear part of the curve as your experimental dilution ratio.
If you are interested in using our ELISA service, you can also send us your sample and we will take care of everything for you. You can check our service details here: bosterbio.com/services/assay-services/ELISA-testing-service
Since you mentioned you have limited samples, our cost effective multiplex ELISA service would fit perfectly for your needs, where we can generate dozens of data points using as little as 25ul sample volume. Information on this service is also in the above link.
Boster Scientific Support
Answered: 2018-10-21
Question
Q: if the enzyme conjugated ANP antibodies are mixed with the substrate, will that convert the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the ANP antigen?
B. Varma
Verified customer
Asked: 2016-10-21
Answer
A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.
Boster Scientific Support
Answered: 2016-10-21
Question
Q: can I use citrate plasma as samples in Human ANP Picokine® ELISA Kit (Catalog # EK1627)?
Verified Customer
Verified customer
Asked: 2015-10-23
Answer
A: Chelating agents such as EDTA, Heparin and Citrate can bind metal ions from the functional domain of ANP causing degradation of its protein structure. ANP may be denatured as a result and may compromise the assay's measurements. The chilating sites could also be too close to the epitopes a must for detection and block the antigen antibody reaction. We have tested the ANP ELISA, treating samples with various anticoagulants and decided that heparin or EDTA can be used for treatment of blood/plasma samples. Do not use other anticoagulents when collecting samples.
Boster Scientific Support
Answered: 2015-10-23
