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Product Info Summary
| SKU: | A01318 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IHC, WB |
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Product info
Product Name
Anti-ANP/Nppa Antibody Picoband®
View all Atrial Natriuretic Peptide/ANP Antibodies
SKU/Catalog Number
A01318
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-ANP/Nppa Antibody Picoband® catalog # A01318. Tested in ELISA, IHC, WB applications. This antibody reacts with Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-ANP/Nppa Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01318)
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E. coli-derived mouse ANP recombinant protein (Position: N25-R122).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A01318 is reactive to Nppa in Mouse, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
17 kDa
Calculated molecular weight
60066 MW
Background of Atrial Natriuretic Peptide/ANP
Atrial natriuretic peptide (ANP), also known as NPPA or PND, is a powerful vasodilator, and a protein (polypeptide) hormone secreted by heart muscle cells. This gene is mapped to 1p36.22. It is involved in the homeostatic control of body water, sodium, potassium and fat (adipose tissue). ANP is released by muscle cells in the upper chambers (atria) of the heart (atrial myocytes) in response to high blood volume. It acts to reduce the water, sodium and adipose loads on the circulatory system, thereby reducing blood pressure. ANP has exactly the opposite function of the aldosterone secreted by the zona glomerulosa in regard to its effect on sodium in the kidney – that is, aldosterone stimulates sodium retention and it generates sodium loss.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A01318 is guaranteed for ELISA, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml
ELISA, 0.1-0.5μg/ml
Positive Control
WB: mouse heart tissue, mouse heart tissue
IHC: rat cardiac muscle tissue, mouse cardiac muscle tissue, rat small intestine tissue
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of ANP using anti-ANP antibody (A01318).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: mouse heart tissue lysates,
Lane 2: mouse heart tissue lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ANP antigen affinity purified polyclonal antibody (Catalog # A01318) at 0.5 μg/mL overnight at 4℃, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ANP at approximately 17KD. The expected band size for ANP is at 17KD.
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Figure 2. IHC analysis of ANP using anti-ANP antibody (A01318).
ANP was detected in paraffin-embedded section of rat cardiac muscle tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-ANP Antibody (A01318) overnight at 4℃. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37℃. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
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Figure 3. IHC analysis of ANP using anti-ANP antibody (A01318).
ANP was detected in paraffin-embedded section of mouse cardiac muscle tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-ANP Antibody (A01318) overnight at 4℃. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37℃. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Click image to see more details
Figure 4. IHC analysis of ANP using anti-ANP antibody (A01318).
ANP was detected in paraffin-embedded section of rat small intestine tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-ANP Antibody (A01318) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Protein Target Info & Infographic
Gene/Protein Information For Nppa (Source: Uniprot.org, NCBI)
Gene Name
Nppa
Full Name
Natriuretic peptides A
Weight
60066 MW
Superfamily
natriuretic peptide family
Alternative Names
Natriuretic peptides A; Prepronatriodilatin; Atrial natriuretic factor; ANF; Atrial natriuretic peptide; ANP; Auriculin-B; Auriculin-A; Atriopeptin-1; Atriopeptin I; Atriopeptin-2; Atriopeptin II; Nppa; Pnd NPPA ANF, ANP, ATFB6, ATRST2, CDD, CDD-ANF, CDP, PND natriuretic peptide A natriuretic peptides A|atrial natriuretic factor prohormone|atrial natriuretic peptide prohormone|atriopeptigen|atriopeptin|cardiodilatin-related peptide|cardionatrin|natriuretic peptide precursor A variant 1|preproANP|preproCDD-ANF|prepronatriodilatin|proANF|proANP
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on Nppa, check out the Nppa Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for Nppa: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-ANP/Nppa Antibody Picoband® (A01318)
Hello CJ!
A01318 has been cited in 1 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Localization of corin and atrial natriuretic peptide expression in human renal segments
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