Click image to see more details
Product Info Summary
| SKU: | M04467 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | Flow Cytometry, IP, WB |
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Product info
Product Name
Anti-AMPK gamma 1 Rabbit Monoclonal Antibody
View all AMPK gamma 1 Antibodies
SKU/Catalog Number
M04467
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-AMPK gamma 1 Rabbit Monoclonal Antibody catalog # M04467. Tested in WB, IP, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-AMPK gamma 1 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M04467)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
GDE-16
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human AMPK gamma 1
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M04467 is reactive to PRKAG1 in Human, Mouse, Rat
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
185 kDa
Calculated molecular weight
37579 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M04467 is guaranteed for Flow Cytometry, IP, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:1000-1:2000
IP 1:20
FC 1:20
Positive Control
WB: HEK293 cell
Validation Images & Assay Conditions
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Western blot analysis of AMPK gamma 1 expression in HEK293 cell lysate.
Protein Target Info & Infographic
Gene/Protein Information For PRKAG1 (Source: Uniprot.org, NCBI)
Gene Name
PRKAG1
Full Name
5'-AMP-activated protein kinase subunit gamma-1
Weight
37579 MW
Superfamily
5'-AMP-activated protein kinase gamma subunit family
Alternative Names
5'-AMP-activated protein kinase subunit gamma-1;AMPK gamma1;AMPK subunit gamma-1;AMPKg;PRKAG1; PRKAG1 AMPKG protein kinase AMP-activated non-catalytic subunit gamma 1 5-AMP-activated protein kinase subunit gamma-1|5-AMP-activated protein kinase, gamma-1 subunit|AMPK gamma-1 chain|AMPK gamma1|protein kinase, AMP-activated, gamma 1 non-catalytic subunit
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on PRKAG1, check out the PRKAG1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for PRKAG1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-AMPK gamma 1 Rabbit Monoclonal Antibody (M04467)
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No publications found for M04467
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Customer Q&As
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3 Customer Q&As for Anti-AMPK gamma 1 Rabbit Monoclonal Antibody
Question
We are currently using anti-AMPK gamma 1 Rabbit Monoclonal antibody M04467 for mouse tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on goat tissues as well?
Verified Customer
Verified customer
Asked: 2020-03-30
Answer
The anti-AMPK gamma 1 Rabbit Monoclonal antibody (M04467) has not been tested for cross reactivity specifically with goat tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in goat you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-03-30
Question
I see that the anti-AMPK gamma 1 Rabbit Monoclonal antibody M04467 works with IP, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2020-03-12
Answer
You can find protocols for IP on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2020-03-12
Question
Do you have a BSA free version of anti-AMPK gamma 1 Rabbit Monoclonal antibody M04467 available?
A. Singh
Verified customer
Asked: 2018-02-02
Answer
I appreciate your recent telephone inquiry. I can confirm that some lots of this anti-AMPK gamma 1 Rabbit Monoclonal antibody M04467 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2018-02-02
